IMPC_API

impc_api is a Python package which provides several helper functions that wrap around the IMPC SOLR API.

The functions in this package are intended for use in a Jupyter Notebook.

Installation Instructions

1. Create a virtual environment (optional but recommended): On Mac:

python3 -m venv .venv
source .venv/bin/activate

2. Install the package: pip install impc_api
3. Install Jupyter: pip install jupyter
4. Run the Jupyter Notebook: jupyter notebook

After executing the command, the Jupyter interface should open in your browser. If it does not, follow the instructions provided in the terminal.

5. Try it out:

Create a Jupyter Notebook and try some of the examples below:

Available functions

The available functions can be imported as:

from impc_api import solr_request, batch_solr_request

1. Solr request

The most basic request to the IMPC solr API

num_found, df = solr_request(
    core='genotype-phenotype', 
    params={
        'q': '*:*',
        'rows': 10, 
        'fl': 'marker_symbol,allele_symbol,parameter_stable_id'
    }
)

a. Facet request

solr_request allows facet requests

num_found, df = solr_request(
    core="genotype-phenotype",
    params={
         "q": "*:*",
         "rows": 0,
         "facet": "on",
         "facet.field": "zygosity",
         "facet.limit": 15,
         "facet.mincount": 1,
    }
)

b. Solr request validation

A common pitfall when writing a query is the misspelling of core and fields arguments. For this, we have included a validate argument that raises a warning when these values are not as expected. Note this does not prevent you from executing a query; it just alerts you to a potential issue.

Core validation

num_found, df = solr_request(
    core='invalid_core',
    params={
        'q': '*:*',
        'rows': 10
    },
    validate=True
)

> InvalidCoreWarning: Invalid core: "genotype-phenotyp", select from the available cores:
> dict_keys(['experiment', 'genotype-phenotype', 'impc_images', 'phenodigm', 'statistical-result'])

Field list validation

num_found, df = solr_request(
    core='genotype-phenotype',
    params={
        'q': '*:*',
        'rows': 10,
        'fl': 'invalid_field,marker_symbol,allele_symbol'
    },
    validate=True
)
> InvalidFieldWarning: Unexpected field name: "invalid_field". Check the spelling of fields.
> To see expected fields check the documentation at: https://www.ebi.ac.uk/mi/impc/solrdoc/

2. Batch Solr Request

batch_solr_request is available for large queries. This solves issues where a request is too large to fit into memory or where it puts a lot of strain on the API.

Use batch_solr_request for:

• Large queries (>1,000,000)
• Querying multiple items in a list
• Downloading data in json or csv format.

Large queries

For large queries you can choose between seeing them in a DataFrame or downloading them in json or csv format.

a. Large query - see in DataFrame

This will fetch your data using the API responsibly and return a Pandas DataFrame

When your request is larger than recommended and you have not opted for downloading the data, a warning will be presented and you should follow the instructions to proceed.

df = batch_solr_request(
    core='genotype-phenotype',
    params={
        'q':'*:*'
    },
    download=False,
    batch_size=30000
)
print(df.head())

b. Large query - Download

When using the download=True option, a file with the requested information will be saved as filename. The format is selected based on the wt parameter. A DataFrame may be returned, provided it does not exceed the memory available on your laptop. If the DataFrame is too large, an error will be raised. For these cases, we recommend you read the downloaded file in batches/chunks.

df = batch_solr_request(
    core='genotype-phenotype',
    params={
        'q':'*:*',
        'wt':'csv'
    },
    download=True,
    filename='geno_pheno_query',
    batch_size=100000
)
print(df.head())

c. Query by multiple values

batch_solr_request also allows to search multiple items in a list provided they belong to them same field. Pass the list to the field_list param and specify the type of fl in field_type.

# List of gene symbols
genes = ["Zfp580", "Firrm", "Gpld1", "Mbip"]

df = batch_solr_request(
    core='genotype-phenotype',
    params={
        'q':'*:*',
        'fl': 'marker_symbol,mp_term_name,p_value',
        'field_list': genes,
        'field_type': 'marker_symbol'
    },
    download = False
)
print(df.head())

This can be downloaded too:

# List of gene symbols
genes = ["Zfp580", "Firrm", "Gpld1", "Mbip"]

df = batch_solr_request(
    core='genotype-phenotype',
    params={
        'q':'*:*',
        'fl': 'marker_symbol,mp_term_name,p_value',
        'field_list': genes,
        'field_type': 'marker_symbol'
    },
    download = True,
    filename='gene_list_query'
)
print(df.head())