CeLEryPy 1.2.1

Leverage spatial transcriptomics data to recover cell locations in single-cell RNA RNA-seq

CeLEry

Leveraging spatial transcriptomics data to recover cell locationsin single-cell RNA-seq with CeLEry

Qihuang Zhang, Jian Hu, Kejie Li, Baohong Zhang, David Dai, Edward B. Lee, Rui Xiao, Mingyao Li*

Single-cell RNA sequencing provides resourceful information to study the cells systematically. However, their locational information is usually unavailable. We present CeLEry, a supervised deep learning algorithm to recover the origin of tissues in assist of spatial transcriptomic data, integrating a data augmentation procedure via variational autoencoder to improve the robustness of methods in the overfitting and the data contamination. CeLEry provides a generic framework and can be implemented in multiple tasks depending on the research objectives, including the spatial coordinates discovery as well as the layer discovery. It can make use of the information of multiple tissues of spatial transcriptomics data. Thorough assessments exhibit that CeLEry achieves a leading performance compared to the state-of-art methods. We illustrated the usage of CeLEry in the discovery of neuron cell layers to study the development of Alzheimer's disease. The identified cell location information is valuable in many downstream analyses and can be indicative of the spatial organization of the tissues.

System Requirements

Python support packages: torch>1.8, pandas>1.4, numpy>1.20, scipy, tqdm, scanpy>1.5, anndata, sklearn

To install package

In the command, input

pip install CeLEryPy

To load the package, input

import CeLEry