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One-step genotyping tools for Nanopore amplicon sequencing

Project description

DAJIN2 is now under development👷

I aim to release a stable version of DAJIN2 by March 2023:crossed_fingers:

New planned features

Experiments

  • support nCATs
  • support PacBio
  • multi-allele phasing

Functions

  • output VCF
  • support multi-processing
  • trim microhomology in BAM

Usability

  • easy installation
    • pypi
    • bioconda
    • docker image
  • support low-/middle-spec laptop
  • support macOS
  • support samples without a reference genome
  • support visualization using igv.js via DAJIN2 view command
  • support graphical user interface using flask via DAJIN2 gui command

Maintainability

  • tests
  • minimize dependencies
    • Python, R, Bash -> Python
    • samtools -> pysam
    • minimap2 -> mappy
    • remove NanoSim
    • remove Tensorflow (GPU computation)

Project details


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