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Object-recognition in images using multiple templates

Project description



Multi-Template-Matching is a package to perform object-recognition in images using one or several smaller template images.
The template and images should have the same bitdepth (8,16,32-bit) and number of channels (single/Grayscale or RGB).
The main function MTM.matchTemplates returns the best predicted locations provided either a score_threshold and/or the expected number of objects in the image.


Using pip in a python environment, pip install Multi-Template-Matching
Once installed, import MTMshould work.
Example jupyter notebooks can be downloaded from the tutorial folder of the github repository and executed in the newly configured python environement.


The wiki section of the repo contains a mini API documentation with description of the key functions of the package.


Check out the jupyter notebook tutorial for some example of how to use the package.
You can run the tutorials online using Binder, no configuration needed ! (click the Binder banner on top of this page).
To run the tutorials locally, install the package using pip as described above, then clone the repository and unzip it.
Finally open a jupyter-notebook session in the unzipped folder to be able to open and execute the notebook.
The wiki section of this related repository also provides some information about the implementation.


If you use this implementation for your research, please cite:

Multi-Template Matching: a versatile tool for object-localization in microscopy images;
Laurent SV Thomas, Jochen Gehrig
bioRxiv 619338; doi:


New github releases are automatically archived to Zenodo.

Related projects

See this repo for the implementation as a Fiji plugin.
Here for a KNIME workflow using Multi-Template-Matching.

Origin of the work

This work has been part of the PhD project of Laurent Thomas under supervision of Dr. Jochen Gehrig at:

Digital Biomedical Imaging Systems AG
Freiburger Str. 3
75179 Pforzheim



This project has received funding from the European Union’s Horizon 2020 research and innovation program under the Marie Sklodowska-Curie grant agreement No 721537 ImageInLife.

ImageInLife MarieCurie

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