Tools to process PacBio sequencing data at molecule level
Project description
This project is about detecting modifications of DNA in PacBio sequencing data stored in the BAM format. The PacBio sequencing data is processed at molecule level: each molecule/ZMW/hole number is analyzed individually.
To learn how to start using this software, have a look at the quickstart section.
Or check the usage section, for more details.
Free software: GNU General Public License v3
Documentation: https://pacbio-data-processing.readthedocs.io
Features
Detection of individual m6A modifications in each molecule.
The single molecule analysis of a BAM file can be performed with a command line interface (CLI) program sm-analysis or using a simple graphical user interface (GUI) program, sm-analysis-gui.
The single molecule analysis can be parallelized to speed up the processing of large BAM files.
An auxiliary executable, bam-filter, is also provided.
Credits
This package was created with Cookiecutter and the palao/cookiecutter-genrepo-pypackage project template.
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