find point mutation in assembled genomes
Project description
PointBlaster
Installation
pip3 install PointBlaster
Dependency
- BLAST+ >2.7.0
- cvmblaster (v0.4.0)
you should add BLAST in your PATH
Blast installation
Windows
Following this tutorial: Add blast into your windows PATH
Linux/Mac
The easyest way to install blast is:
conda install -c bioconda blast
Usage
1. Initialize reference database
After finish installation, you should first initialize the reference database using following command
PointBlaster -init
Usage: PointBlaster -i <genome assemble directory> -s <species for point mutation detection> -o <output_directory>
Author: Qingpo Cui(SZQ Lab, China Agricultural University)
optional arguments:
-h, --help show this help message and exit
-i I <input_path>: the PATH to the directory of assembled genome
files. Could not use with -f
-f F <input_file>: the PATH of assembled genome file. Could not
use with -i
-o O <output_directory>: output PATH
-s S <species>: optional var is [salmoenlla, campylobacter],
other species will be supported soon
-minid MINID <minimum threshold of identity>, default=90
-mincov MINCOV <minimum threshold of coverage>, default=60
-list <show species list>
-t T <number of threads>: default=8
-v, --version <display version>
-init <initialize the point mutationdatabase>
Project details
Release history Release notifications | RSS feed
Download files
Download the file for your platform. If you're not sure which to choose, learn more about installing packages.
Source Distribution
PointBlaster-0.1.5.tar.gz
(730.5 kB
view hashes)
Built Distribution
PointBlaster-0.1.5-py3-none-any.whl
(257.7 kB
view hashes)
Close
Hashes for PointBlaster-0.1.5-py3-none-any.whl
Algorithm | Hash digest | |
---|---|---|
SHA256 | 3a5af77c8a0936e76c0d6d7302e4a828707e8ca38bd263ed8f0b3eedc5992f3d |
|
MD5 | e887ef53a5b7503f6286e73f07888217 |
|
BLAKE2b-256 | 741b2dbe67b9274c962ca1a779be47bb0dd08e73308fe19c595005f15e6909bc |