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A Python package to analyze flouresence intermittency data of quantum dots

Project description

QuantumBlink

INTRODUCTION

QunatumBlink is a python module developed by Anoop A Nair, under the supervison of Vishnu E K,Ph.D [@ K.G.T. Labs]. It helps in the analysis of flouresence intermittency data obtained from the MT-300 single photon detector. The module when provided with the Intensity vs Time trace derives the ON/OFF event PDFs and the correlations in data indicating the memory effect.

HOW TO INSTALL

The Quantum Blink module depends on the numpy module for most of it's functionality.

To Install numpy use:

pip install numpy

To install QuantumBlink use:

pip install QuantumBlink

To install QuantumBlink of a particular version say 1.x use:

pip install QuantumBlink==1.x

HOW TO USE

The module can be imported after installation using:

import QuantumBlink as qb

The csv file should contain two columns the first one being the time and second one being the intesity values.

KEYWORDS and METHODS

> Data Acquisition

>> Intensity data

IntensityData  =  IntensityDataAcquire(PATH)

1.INPUT

  • PATH = "the path to the csv data file"

2.OUTPUT

  • IntensityData = array of intesity values

> The ON/OFF Events

>> ON-OFF durations

Positives , Negatives =  Power_dist_one(PATH,Threshold,exptime)

1.INPUT

  • PATH = "the path to the csv data file".
  • Threshold = This specifies the instensity level above which events are treated as positve and below which events are treated as negative.
  • exptime = The time interval between each consecutive event.

2.OUTPUT

  • Positives = array of ON time durations
  • Negatives = array of OFF time durations

>> OFF-time distribution

Pdf_accept_off,Offtime =  Offtime_pdf(PATH,Threshold,exptime)

1.INPUT

  • PATH = "the path to the csv data file".
  • Threshold = This specifies the instensity level above which events are treated as positve and below which events are treated as negative.
  • exptime = The time interval between each consecutive event.

2.OUTPUT

  • Pdf_accept_off = The probability distribution of OFF-time durations
  • Offtime = array of distinct OFF-time durations

>> ON-time distribution

Pdf_accept_on,Ontime =  Ontime_pdf(PATH,Threshold,exptime)

1.INPUT

  • PATH = "the path to the csv data file".
  • Threshold = This specifies the instensity level above which events are treated as positve and below which events are treated as negative.
  • exptime = The time interval between each consecutive event.

2.OUTPUT

  • Pdf_accept_on = The probability distribution of ON-time durations
  • Ontime = array of distinct ON-time durations

> Event Duration info

>> ON time ratio

On_ratio =  OnTimeFraction(PATH,Threshold,exptime)

1.INPUT

  • PATH = "the path to the csv data file".
  • Threshold = This specifies the instensity level above which events are treated as positve and below which events are treated as negative.
  • exptime = The time interval between each consecutive event.

2.OUTPUT

  • On_ratio = The fraction of ON events.

>> OFF time ratio

Off_ratio =  OffTimeFraction(PATH,Threshold,exptime)

1.INPUT

  • PATH = "the path to the csv data file".
  • Threshold = This specifies the instensity level above which events are treated as positve and below which events are treated as negative.
  • exptime = The time interval between each consecutive event.

2.OUTPUT

  • Off_ratio = The fraction of OFF events.

>> ON-OFF ratio

ON_OFF_ratio =  OnOffRatio(PATH,Threshold,exptime)

1.INPUT

  • PATH = "the path to the csv data file".
  • Threshold = This specifies the instensity level above which events are treated as positve and below which events are treated as negative.
  • exptime = The time interval between each consecutive event.

2.OUTPUT

  • ON_OFF_ratio = The ratio of ON to OFF events.

>> OFF-ON ratio

OFF_ON_ratio =  OffOnRatio(PATH,Threshold,exptime)

1.INPUT

  • PATH = "the path to the csv data file".
  • Threshold = This specifies the instensity level above which events are treated as positve and below which events are treated as negative.
  • exptime = The time interval between each consecutive event.

2.OUTPUT

  • OFF_ON_ratio = The ratio of OFF to ON events.

>> TOTAL ONtime

TOTAL_ONTIME =  TotalOnTime(PATH,Threshold,exptime)

1.INPUT

  • PATH = "the path to the csv data file".
  • Threshold = This specifies the instensity level above which events are treated as positve and below which events are treated as negative.
  • exptime = The time interval between each consecutive event.

2.OUTPUT

  • TOTAL_ONTIME = The total time occupied by ON events.

>> TOTAL OFFtime

TOTAL_OFFTIME =  TotalOffTime(PATH,Threshold,exptime)

1.INPUT

  • PATH = "the path to the csv data file".
  • Threshold = This specifies the instensity level above which events are treated as positve and below which events are treated as negative.
  • exptime = The time interval between each consecutive event.

2.OUTPUT

  • TOTAL_OFFTIME = The total time occupied by OFF events.

> Mean, Min, Max intensities

>> The min, max and average intesity of tthe data

AverageIntensity = AverageIntensity(PATH)

1.INPUT

  • PATH = "the path to the csv data file".

2.OUTPUT

  • AverageIntensity = The Average intensity calculated from the data.
MaxIntensity = MaxIntensity(PATH)

1.INPUT

  • PATH = "the path to the csv data file".

2.OUTPUT

  • MaxIntensity = The maximum intensity calculated from the data.
MinIntensity = MinIntensity(PATH)

1.INPUT

  • PATH = "the path to the csv data file".

2.OUTPUT

  • MinIntensity = The minimum intensity calculated from the data.

>> The average intesity between two intensity levels

Intensity_average = AverageIntensityBetween(PATH,Threshold1,Threshold2)

1.INPUT

  • PATH = "the path to the csv data file".
  • Threshold1 = The upper threshold
  • Threshold2 = The lower threshold

2.OUTPUT

  • Intensity_average = The average intensity between the upper and lower threshold

> Memory Effect

>> ON-OFF Correlation

XX,YY,R1 =  OnOffCorr(PATH,Threshold,exptime)

1.INPUT

  • PATH = "the path to the csv data file".
  • Threshold = This specifies the instensity level above which events are treated as positve and below which events are treated as negative.
  • exptime = The time interval between each consecutive event.

2.OUTPUT

  • XX = array of ON/OFF events .
  • YY = array of ON/OFF events .
  • R1 = The correlation coefficient.
X_x_log,Y_y_log,R1 = OnOffCorrLog(PATH,Threshold,exptime)

1.INPUT

  • PATH = "the path to the csv data file".
  • Threshold = This specifies the instensity level above which events are treated as positve and below which events are treated as negative.
  • exptime = The time interval between each consecutive event.

2.OUTPUT

  • X_x_log = array of log value of ON/OFF events .
  • Y_y_log = array of log value of ON/OFF events .
  • R1 = The correlation coefficient.

>> ON-ON Correlation

XX,YY,R1 OnOnCorr(PATH,Threshold,exptime)

1.INPUT

  • PATH = "the path to the csv data file".
  • Threshold = This specifies the instensity level above which events are treated as positve and below which events are treated as negative.
  • exptime = The time interval between each consecutive event.

2.OUTPUT

  • XX = array of ON events .
  • YY = array of ON events .
  • R1 = The correlation coefficient.
X_x_log,Y_y_log,R1 = OnOnCorrLog(PATH,Threshold,exptime)

1.INPUT

  • PATH = "the path to the csv data file".
  • Threshold = This specifies the instensity level above which events are treated as positve and below which events are treated as negative.
  • exptime = The time interval between each consecutive event.

2.OUTPUT

  • X_x_log = array of log value of ON events .
  • Y_y_log = array of log value of ON events .
  • R1 = The correlation coefficient.

>> OFF-OFF Correlation

XX,YY,R1 =  OffOffCorr(PATH,Threshold,exptime)

1.INPUT

  • PATH = "the path to the csv data file".
  • Threshold = This specifies the instensity level above which events are treated as positve and below which events are treated as negative.
  • exptime = The time interval between each consecutive event.

2.OUTPUT

  • XX = array of OFF events .
  • YY = array of OFF events .
  • R1 = The correlation coefficient.
X_x_log,Y_y_log,R1 OffOffCorrLog(PATH,Threshold,exptime)

1.INPUT

  • PATH = "the path to the csv data file".
  • Threshold = This specifies the instensity level above which events are treated as positve and below which events are treated as negative.
  • exptime = The time interval between each consecutive event.

2.OUTPUT

  • X_x_log = array of log value of OFF events .
  • Y_y_log = array of log value of OFF events .
  • R1 = The correlation coefficient. """

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