Enzyme selection for DNA verification and identification
BandWitch is a Python library for the planning and analysis of restriction experiments in DNA assembly operations. BandWitch implements method to select the best enzyme(s) to validate or identify DNA assemblies. It also provides report generation methods to automatically validate/identify assemblies from experimental data.
You can try BandWitch’s enzyme suggestion feature in this web demo, and the sequence validation (from AATI fragment analyzer files) in this other demo
pip install bandwitch
Enzyme suggestion: http://cuba.genomefoundry.org/digestion-selector
Fragment analysis validation: http://cuba.genomefoundry.org/analyze-digests
License: MIT, Copyright Edinburgh Genome Foundry
Enzymes selected by bandwitch to obtain clear, optimal patterns for all tested DNA constructs:
Enzymes selected by bandwitch to obtain significant differences between the patterns of the tested constructs, so that a construct can be identified by its pattern:
More biology software
BandWitch is part of the EGF Codons synthetic biology software suite for DNA design, manufacturing and validation.
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