barseq

Python package for analyzing barseq data.

Installation

pip install git+https://github.com/mjmlab/barseq.git@master

Usage

barseq -i <directory of sequencing reads> -b <barcode file> -e <experiment name>

-i / --input

• Directory with Illumina reads in either fastq or fastq.gz.

-b / --barcodes

• CSV file with barcode and correspondent gene names.

-e / --experiment

• Name of experiment, it is used for creating results folder.

Files Needed

Directory of Illumina reads [-i] for analyzing. Can be either fastq or fastq.gz format

@M06026:87:000000000-D69HY:1:1102:15909:1336 1:N:0:TGACCA
CTCTAGAAAGTATAGGAACTTCAGGGCCATTTATATACCTTCCACTCTTCAACCGTGTCTTGACTTGACCTGGATGTCTCTACTGCTGTCATGCTACGTAGCTCATGCTACGTCGATCTAGTCGATGCATGCTAGCTGATCGACTCTCTTC
+
A#>>>3AA2DD>FBFGBFBFBFDDFGGAAEEEHDHFFFDDDBDGDFDDDDDADFGFFDDBFFEBFD5DFEEBBADABFGFGBBFGDD5BF3F43B3F1/11B144BGEBF@BBFB0B0BBFBBBBBBBB?E/FGFBB?/???B???/?FGG

Barcode file [-b] with gene names. Needs to be in CSV format.

Barcode,Gene
ATGAAGACTGTTGCCGTA,WT
CACGACGCCCTCCGCGGA,gene1
ACTATTACGCAAAATAAT,gene2
ATGGAAGATATTATTATT,gene3
CCTCTCCAACCGGGTCTG,gene4
CCCGGTCGCCTAGCCCCG,gene5
GGCCCCCCGCCCGTCCCC,gene6
GGATCACTGCTAGCGTAT,gene7
CCTGCAGCAGCGGCCCGC,gene8
ACACATGCAGACATAGAG,gene9
CGCGCCATCCGCCGCCCA,gene10
AATATTCAGATGGGACGT,gene11

Output

results/ directory

• _results.csv: barcode counts found in each sequence file.

Barseq Workflow