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Allows reading Bio-Rad *.1sc image/analysis files.

Project description

Python Package to read, parse, convert *.1sc files generated by Bio-Rad Quantity One software.

Also installs bio1sc2tiff command-line executable to convert *.1sc files to *.tif images.

An optional dependency: numpy. If present, biorad1sc_reader will run 4x to 5x faster.

Current Status

  • Version 0.1

  • “Alpha” quality software
    • API subject to change

    • No known problems in operation.

  • Installs command-line executable: bio1sc2tiff (type ‘bio1sc2tiff –help’)

  • Reading Image Data OK

  • Saving Image from file to new TIFF file OK

  • Reading Image Metadata from Data Block 7 OK
    • Scanner Name: <Scanner Name>

    • Number of Pixels: (<img_size_x> x <img_size_y>)

    • Image Area: (<img_size_x> mm x <img_size_y> mm)

    • Scan Memory Size: <total_img_size> Kb

    • Old file name: <old_file_name>

    • New file name: <new_file_name>

    • <File Directory>

    • New Image Acquired

    • Save As…

    • Quantity One <Quantity One Version String>

  • python2 (lack of) support
    • So far code works with python2 if you remove the maxsplit argument from split

    • I’m currently unwilling to support python2

    • If you’d like python2 support, it’s DIY. :)

Documentation

Examples

# setup reader with input file
import bio1sc_reader
myreader = bio1sc_reader.Reader("my_biorad_file.1sc")

# get dict of metadata in Data Block 7 of 1sc file
my_img_metadata = myreader.get_img_metadata()

# Different options for writing image data out as a TIFF file
myreader.save_img_as_tif("unscaled_brightness.tif")
myreader.save_img_as_tif("unscaled_inverted_brightness.tif", invert=True)
myreader.save_img_as_tif_sc("scaled_brightness.tif")
myreader.save_img_as_tif_sc("scaled_brightness_more.tif", scale=0.8)
myreader.save_img_as_tif_sc("scaled_inverted_brightness.tif", invert=True)

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