Welcome to Blacktie!
This package is functional but under heavy development so you may want to periodically
pull the latest code from the repo at git://github.com/xguse/blacktie.git.
For the **LATEST** version pull the 'develop' branch.
**I seek collaboration and contributions to improve this tool.**
**PLEASE** take it and run with it, but send me your progress!
Its goal is to **simplify the integration of all the
input/output streams of the tophat/cufflinks workflow** into a single yaml based config
file and automate as much of everything else as possible so that once the config file
is filled out, **the entire process of analysing a multi-condition RNA-seq experiment can
be run with a single command.**
I have not had time to fill in ALL the doc strings but updating them regularly.
(Current version of the docs can be found here: http://xguse.github.com/blacktie/)
If you want to use/contribute before the docs are complete, please feel free to contact me at
firstname.lastname@example.org for guidance.
If you find issues, bugs, or have feature requests, please go here to submit them: https://github.com/xguse/blacktie/issues
.. raw:: html
<iframe src="http://wl.figshare.com/articles/714149/embed?show_title=1" width="568" height="200" frameborder="0"></iframe>
To credit the use of blacktie please cite the poster using the DOI link provided.
*Introducing Blacktie: a simpler way to do RNA-seq using Tophat/Cufflinks/CummeRbund*. Augustine Dunn. figshare.
.. code-block:: none
usage: blacktie [-h] [--version]
This script reads options from a yaml formatted file and organizes the
execution of tophat/cufflinks runs for multiple condition sets.
config_file Path to a yaml formatted config file containing setup
options for the runs.
-h, --help show this help message and exit
--version Print version number.
Which program do you want to run? (default: tophat)
--hide-logs Make your log directories hidden to keep a tidy
'looking' base directory. (default: False)
--no-email Don't send email notifications. (default: False)
1) 'analyze': run the analysis pipeline. 2) 'dry_run':
walk through all steps that would be run and print out
the command lines; however, do not send the commands
to the system to be run. 3) 'qsub_script': generate
bash scripts suitable to be sent to a compute
cluster's SGE through the qsub command. (default:
.. _Buildout: http://www.buildout.org/
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*Release date: 2013-07-17*
* version number system adopted to conform to PEP386
* This is a hot fix to squash a bug described in github issue: https://github.com/xguse/blacktie/issues/10
* **in short:** on a Mac ``pprocess`` complained "AttributeError: 'module' object has no attribute 'poll'" when trying to set up a queue.
* the quick fix is to look for that exception and continue without ``pprocess`` if encountered to avoid the fatal exception.
*Release date: 2013-05-15*
* git tag: 'v0.2.1'
* added new script named blacktie-cummerbund to run cummeRbund
* added new class in calls.py CummerbundCall to use blacktie-cummerbund script to add cummeRbund plots to blacktie script
* checks for R and rpy2 installations
* if cummeRbund R library not found, it walks you through installing it
* ``src/blacktie/utils/calls.py``: - fixed _flag_out_dir() so that if the outdir has not been created yet it gracefully moves on
* ``examples/blacktie_config_example.yaml``: - added cummerbund_options
* ``requirements.txt``: - added rpy2
* updated docs
*Release date: 2013-04-19*
* git tag: 'v0.2.0rc1'
* Added support for handling biological replicates in cuffdiff runs.
* Major changes to yaml config:
* condition_queue[index].group_id --> condition_queue[index].experiment_id
* addition of condition_queue[index].replicate_id to track replicate data
* condition_queue[index].name now represents description of an 'experiemental condition' and will be shared by replicates.
*Release date: 2013-03-21*
* git commit SHA digest: 9d7c02d5e7f4ec8970bb2291c3eb6ff8f4dbe542
* git tag: 'v0.1.1'
*Release date: 2013-03-20*
* git commit SHA digest: 808c11fb396c71af55ff690fa6f2e628e5ffd758
* git tag: 'v0.1-dev'
* First uploaded to PyPI
* This verion is still under active development.
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