To get taxa information of sequences from BOLD system
Project description
bold_identification
1 Introduction
see https://github.com/linzhi2013/bold_identification
.
This is a package which can get the taxonomy information of sequences from BOLD http://www.boldsystems.org/index.php.
To get the taxonomy information of a sequence from BOLD, what we usually do is: (1) open the website http://www.boldsystems.org/index.php/IDS_OpenIdEngine
with a browser; (2) Choose a database; (3) input the sequence (4) click submit
and wait for the result. (5) Copy the taxonomy information from the result page.
bold_identification
actually does the same things as above, but it does such a thing automatically for you, and makes life easier.
Currently, bold_identification
only runs on Mac OS, Windows 64bit, Linux.
But be ware, only the Chrome browser works on Windows, while FireFox doesn't.
2 Installation
pip3 install bold_identification
There will be a command bold_identification
created under the same directory as your pip3
command.
3 Usage
run bold_identification
usage: bold_identification [-h] -i <str> -o <str>
[-d {COX1,COX1_SPECIES,COX1_SPECIES_PUBLIC,COX1_L640bp,ITS,Plant}]
[-n <int>] [-b {Firefox,Chrome}]
[-t <int>] [-r <int>]
To identify taxa of given sequences. Some sequences can fail to get taxon
information, which can be caused by TimeoutException if your network to the
BOLD server is bad. Those sequences will be output in the file
'*.TimeoutException.fasta'. You can: 1) run another searching for those
sequences directly; 2) change the browser (-b option); 3) lengthen the time to
wait for each query (-t option); 4) increase submission times (-r option) for
a sequence. By mengguanliang@genomics.cn. See
https://github.com/linzhi2013/bold_identification.
optional arguments:
-h, --help show this help message and exit
-i <str> input fasta file
-o <str> outfile
-d {COX1,COX1_SPECIES,COX1_SPECIES_PUBLIC,COX1_L640bp,ITS,Plant}
database to search [COX1]
-n <int> how many first top hits will be output. [1]
-b {Firefox,Chrome} browser to be used [Chrome]
-t <int> the time to wait for a query [30]
-r <int> Maximum submission time for a sequence, useful for
handling TimeOutException. [2]
4 Problems
Cannot download the browsers
This can happen when your network is not good.
Solution:
Download the executable driver file manaully, then extract the executable and put it on the drivers
directory. See more details output by bold_identification
when you run into this problem.
Browser doen't work
Sometimes it happens to me. And I don't know why. I guess it is because the browser driver is not so stable.
Solution:
Try another browser with the -b
option.
5 Citation
Currently I have no plan to publish bold_identification
.
Project details
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