breakfast 0.3.2

breakfast: fast putative outbreak cluster and infection chain detection using SNPs

breakfast - FAST outBREAK detection and sequence clustering

breakfast is a simple and fast script developed for clustering SARS-CoV-2 genomes using precalculated sequence features (e.g. nucleotide substitutions) from covSonar or Nextclade.

This project is under development and in experimental stage

Installation

Installation using pip

$ pip install breakfast

System Dependencies

breakfast runs under Python 3.10 and later. The base requirements are networkx, pandas, numpy, scikit-learn, click, and scipy.

Install using conda

We recommend using conda for installing all necessary dependencies:

conda env create -n sonar -f covsonar/sonar.env.yml
conda env create -n breakfast -f breakfast/envs/sc2-breakfast.yml

Example Command Line Usage

Simple test run

conda activate breakfast
breakfast/src/breakfast.py \
   --input-file breakfast/test/testfile.tsv  \
   --max-dist 1 \
   --outdir test-run/

You will find your results in test-run/cluster.tsv, which should be identical to breakfast/test/expected_clusters_dist1.tsv

1) covSonar + breakfast

Sequence processing with covSonar

conda activate sonar
covsonar/sonar.py add -f genomes.fasta --db mydb --cpus 8
covsonar/sonar.py match --tsv --db mydb > genomic_profiles.tsv

Clustering with a maximum SNP-distance of 1 and excluding clusters below a size of 5 sequences

conda activate breakfast
breakfast/src/breakfast.py \
   --input-file genomic_profiles.tsv \
   --max-dist 1 \
   --min-cluster-size 5 \
   --outdir covsonar-breakfast-results/

2) Nextclade + breakfast

Sequence processing with Nextclade CLI.

conda install -c bioconda nextclade
nextclade dataset get --name 'sars-cov-2' --output-dir 'data/sars-cov-2'
nextclade \
   --in-order \
   --input-fasta genomes.fasta \
   --input-dataset data/sars-cov-2 \
   --output-tsv output/nextclade.tsv \
   --output-tree output/nextclade.auspice.json \
   --output-dir output/ \
   --output-basename nextclade

Alternatively, you can also use Nextclade Web to process your fasta and export the genomic profile as "nextclade.tsv".

Clustering with a maximum SNP-distance of 1 and excluding clusters below a size of 5 sequences. Since the input tsv of Nextclade looks a little different from the covSonar tsv, you need to specify the additional parameters --id-col, --clust-col and --sep2 for identifying the correct columns.

conda activate breakfast
breakfast/src/breakfast.py \
   --input-file output/nextclade.tsv \
   --max-dist 1 \
   --min-cluster-size 5 \
   --id-col "seqName" \
   --clust-col "substitutions" \
   --sep2 "," \
   --outdir nextclade-breakfast-results/

Parameter description

Parameter	Type	Required	Default	Description
--input-file	String	✅	'genomic_profiles.tsv.gz'	Path of the input file (in tsv format)
--max-dist	Integer		1	Two sequences will be grouped together, if their pairwise edit distance does not exceed this threshold
--min-cluster-size	Integer		2	Minimum number of sequences a cluster needs to include to be defined in the result file
--id-col	String		'accession'	Name of the sequence identifier column of the input file
--clust-col	String		'dna_profile'	Name of the mutation profile column of the input file
--var-type	String		'dna'	Specify if DNA or AA substitutions are used for the mutation profiles
--sep	String		'\t'	Input file separator
--sep2	String		' '	Secondary clustering column separator (between each mutation)
--outdir	String		'output/'	Path of output directory
--trim-start	Integer		264	Bases to trim from the beginning
--trim-end	Integer		228	Bases to trim from the end
--reference-length	Integer		29903	Length of reference genome (defaults to NC_045512.2)
--skip-del	Bool		TRUE	Deletions will be skipped for calculating the pairwise distance of your input sequences.
--skip-ins	Bool		TRUE	Insertions will be skipped for calculating the pairwise distance of your input sequences.
--input-cache	Integer		None	Path to import results from previous run
--output-cache	String		None	Path to export results which can be used in the next run to decrease runtime.
--help	N/A		N/A	Show this help message and exit
--version	N/A		N/A	Show version and exit