Bisulfite Sequencing Processing Platform
Project description
BSBolt (BiSulfite Bolt)
A fast and safe bisulfite sequencing processing platform
BiSuflite Bolt (BSBolt); a fast and scalable bisulfite sequencing processing platform. BSBolt is an integrated analysis platform that offers support for bisulfite sequencing read simulation, alignment, methylation calling, data aggregation, and data imputation. BSBolt has been validated to work with a wide array of bisulfite sequencing data,including whole genome bisulfite sequencing (WGBS), reduced representative bisulfite sequencing data (RRBS), and targeted methylation sequencing data. BSBolt utilizes forked versions of BWA and WGSIM for read alignment and read simulation respectively. BSBolt is released under the MIT license.
Documentation
BSBolt documentation can be found at bsbolt.readthedocs.io.
Installation
BSBolt alignment is performed using a modified version of BWA. Read simulation is carried out using a modified version of wgsim.
Install through PyPi
pip3 install BSBolt --user
Install from source
git clone git@github.com:NuttyLogic/BSBolt.git
cd BSBolt-master
python3 setup.py install .
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