cirro 1.10.1

CLI tool and SDK for interacting with the Cirro platform

Cirro Client

A Python 3.10+ library for the Cirro platform.

Installation

You can install Cirro using pip:

pip install cirro

or you can install the main branch of the repo by running:

pip install git+https://github.com/CirroBio/Cirro-client.git

To enable pipeline configuration you need to install extras using:

pip install cirro[nextflow]      # just nextflow pipeline configuration support
pip install cirro[wdl]           # just wdl pipeline configuraiton support
pip install cirro[nextflow,wdl]  # both nextflow and wdl pipeline configuration support

NOTE: Configuring Nextflow pipelines also requires a local installation of nextflow.

Authentication

Upon first use, the Cirro client will ask you what Cirro instance to use and if you would like to save your login information. It will then give you a link to authenticate through the web browser.

You can change your Cirro instance by running cirro configure and selecting the desired instance.

If you need to change your credentials after this point, and you've opted to save your login, please see the clearing saved login section.

Command Line Usage

Downloading a dataset:

Usage: cirro download [OPTIONS]

  Download dataset files

Options:
  --project TEXT         Name or ID of the project
  --dataset TEXT         ID of the dataset
  --file TEXT            Relative path of the file(s) to download (optional, can be used multiple times)
  --data-directory TEXT  Directory to store the files
  -i, --interactive      Gather arguments interactively
  --help                 Show this message and exit.

$ cirro download --project "Test Project 1" --dataset "test" --data-directory "~/download"

Uploading a dataset:

Usage: cirro upload [OPTIONS]

  Upload and create a dataset

Options:
  --name TEXT                  Name of the dataset
  --description TEXT           Description of the dataset (optional)
  --project TEXT               Name or ID of the project
  --data-type, --process TEXT  Name or ID of the data type (--process is deprecated)
  --data-directory TEXT        Directory you wish to upload
  --file TEXT                  Relative path of the file(s) to upload (optional, can be used multiple times)
  -i, --interactive            Gather arguments interactively
  --include-hidden             Include hidden files in the upload (e.g., files starting with .)
  --help                       Show this message and exit.

$ cirro upload --project "Test Project 1" --name "test" --file "sample1.fastq.gz" --file "sample2.fastq.gz" --data-directory "~/data" --data-type "Paired DNAseq (FASTQ)" 

Validating that a dataset matches a local folder

Usage: cirro validate [OPTIONS]

  Validate that the contents of a local folder match those of a dataset in Cirro

Options:
  --dataset TEXT               Name or ID of the dataset
  --project TEXT               Name or ID of the project
  --data-directory TEXT        Local directory you wish to validate
  -i, --interactive            Gather arguments interactively
  --help                       Show this message and exit.

$ cirro validate --project "Test Project 1" --dataset "test" --data-directory "~/data"

Uploading a reference

Usage: cirro upload-reference [OPTIONS]

  Upload a reference to a project

Options:
  --name TEXT            Name of the reference
  --reference-type TEXT  Type of the reference (e.g., Reference Genome (FASTA))
  --project TEXT         Name or ID of the project
  --reference-file TEXT  Location of reference file(s) to upload (can be used multiple times)
  -i, --interactive      Gather arguments interactively
  --help                 Show this message and exit.

Listing datasets:

Usage: cirro list-datasets [OPTIONS]

  List available datasets

Options:
  --project TEXT         ID of the project
  -i, --interactive      Gather arguments interactively
  --help                 Show this message and exit.

Configuring a pipeline

Usage: cirro create-pipeline-config [OPTIONS]

  Create pipeline configuration files

Options:
  -p, --pipeline-dir DIRECTORY  Directory containing the pipeline definition
                                files (e.g., WDL or Nextflow)  [default: .]
  -e, --entrypoint TEXT         Entrypoint WDL file (optional, if not
                                specified, the first WDL file found will be
                                used). Ignored for Nextflow pipelines.
  -o, --output-dir TEXT         Directory to store the generated configuration
                                files  [default: .cirro]
  -i, --interactive             Gather arguments interactively
  --help                        Show this message and exit.

It is highly recommended that:

• Nextflow pipelines utilize a nextflow_schema.json file. (If your pipeline originates from NF-Core, this should already be the case.)
• WDL pipelines are defined in WDL v1.0 or higher and explicitly define an input section in the root-level workflow.

Interactive Commands

When running a command, you can specify the --interactive flag to gather the command arguments interactively.

Example:

$ cirro upload --interactive
? What project is this dataset associated with?  Test project
? Enter the full path of the data directory  /shared/biodata/test
? Please confirm that you wish to upload 20 files (0.630 GB)  Yes
? What type of files?  Illumina Sequencing Run
? What is the name of this dataset?  test
? Enter a description of the dataset (optional)

Python Usage

See the following set of Jupyter notebooks that contain examples on the following topics:

Jupyter Notebook	Topic
Introduction	Installing and authenticating
Uploading a dataset	Uploading data
Downloading a dataset	Downloading data
Interacting with a dataset	Calling data and reading into tables
Analyzing a dataset	Running analysis pipelines
Using references	Managing reference data
Advanced usage	Advanced operations

R Usage

Jupyter Notebook	Topic
Downloading a dataset in R	Reading data with R

Advanced Usage

View the API documentation for this library here.

Supported environment variables

Name	Description	Default
CIRRO_HOME	Local configuration directory	~/.cirro
CIRRO_BASE_URL	Base URL of the data portal

Configuration

The cirro configure command creates a file in CIRRO_HOME called config.ini.

You can set the base_url property in the config file rather than using the environment variable.

The transfer_max_retries configuration property specifies the maximum number of times to attempt uploading a file to Cirro in the event of a transfer failure. When uploading files to Cirro, network issues or temporary outages can occasionally cause a transfer to fail. It will pause for an increasing amount of time for each retry attempt.

The default hashing algorithm for files is CRC64. In many cases, CRC64 is sufficient to ensure data integrity upon upload.

[General]
base_url = cirro.bio
transfer_max_retries = 15

Clearing saved login

You can clear your saved login information by removing the ~/.cirro/token.dat file from your system or by running cirro configure and selecting No when it asks if you'd like to save your login information.