CytoSmartOpenAPI 1.3.0

This is a python wrapper for the CytoSMART windows app to use it headless

CytoSMART Open API

This is a Python wrapper for the CytoSMART Windows app to use it headless. The package will only work on Windows 10 and above.

This package was formally known as luxconnector

Warranty

⚠️ Hardware warranty is void by using this open API ⚠️:

Using the CytoSmartOpenAPI means you will have NO hardware warranty (see license).

This is because our devices are made to handle the normal usage (the GUI app). It also includes additionally bought warranty. Only if your additionally bought warranty implicitly includes the Open API usage you will have warranty.

Installation

To install this package follow these steps:

Step 1: Drivers

Make sure you had a CytoSmart application installed.

Recommended is the cell counter given it does not restart itself after closing. This is needed to have all the correct drivers installed. Uninstall the app afterwards. If the app is on the same machine the Open API might connect with the wrong one.

Step 2: pip install

pip install CytoSmartOpenAPI

Initialization

Make sure at least one CytoSMART device is physically connected to your computer via a USB3 port. The CytoSmartOpenAPI is an object that maintains the connection with the device. To create the object use the following code:

from CytoSmartOpenAPI import CytoSmartOpenAPI
connector = CytoSmartOpenAPI(number_of_devices=2, warranty=False)

⚠️ You are voiding your warranty by using this package

NOTE: Make sure number_of_devices does not exceed the number of devices you have physically connected. Otherwise the CytoSmartOpenAPI will look for the missing devices forever.

Getting serial numbers

Each physical device has a serial number. This number is needed to send commands to a specific device.

serial_numbers = connector.get_all_serial_numbers()

Getting a single image

When you want a single image taken at this moment use get_image. This will return the image as a pillow image. You need to give the serial number of the device you want to target.

img = connector.get_image(serial_number)

or

img = connector.get_image(serial_numbers[index])

Changing the focus

This function will change the focus for the CytoSmartOpenAPI object. Every image taken after this function will have the new focus.

The focus is in the range 0 until 1. (0.0 and 1.0 are valid entries)

You need to give the serial number of the device you want to target.

connector.set_focus(serial_number, 0.5)
img1 = connector.get_image(serial_number) # Image with focus of 0.5
connector.set_focus(serial_number, 0.7)
img2 = connector.get_image(serial_number) # Image with focus of 0.7
img3 = connector.get_image(serial_number) # Image with focus of 0.7

Auto focus

The device goes over multiple possible focuses to find the best focus. It will use image analysis to determine how well the image is in focus.

# Fast but goes over a limited range to cover most manufactured cell counting slides
connector.do_autofocus(serial_number, "slide")
# Fastest: goes over the limited range where cells can be in focus on the CytoSMART cell counting slide
connector.do_autofocus(serial_number, "CSslide")
# Slow, but goes over the full range of possible focusses: suitable for any vessel that works with the CytoSMART device
connector.do_autofocus(serial_number, "other")

Getting the temperature

This function returns the temperature in Celsius of the device.

You need to give the serial number of the device you want to target.

temperature = connector.get_temperature(serial_number)

Change active camera (fluorescence)

To use fluorescence you will need to change the active camera. The camera can be set to 3 different values, RED, GREEN, or BRIGHTFIELD. This will only work if your device is an FL device otherwise only BRIGHTFIELD is available.

connector.set_active_camera(serial_number, "RED")
connector.set_active_camera(serial_number, "GREEN")
connector.set_active_camera(serial_number, "BRIGHTFIELD")

Change camera setting

Each camera has its own settings. Not all settings are available for BRIGHTFIELD.

• exposure: The time in milliseconds that the camera is detecting light. (Lux and Exact devices only; for Omni devices use flash duration)
• gain: The multiplication of the camera. (Fluo only) If very little light goes into the camera sensor make sure the gain is high.
• brightness: Strength of the led when it is on. (Fluo only)
• focus_offset: The difference in focus between brightfield and fluo. If focus is set to 0.4 and focus_offset for RED is set to 0.1 RED focus is 0.5 (Fluo only)

connector.set_camera_settings(serial_number, "RED", 500, gain=30, brightness=5000, focus_offset=0.0)
connector.set_camera_settings(serial_number, "BRIGHTFIELD", 10)

Change flash duration (Omni only)

The Omni cannot change the exposure time but can change the time the led is on. This can be done with set_flash_duration. It will set the duration between 40 and 250 μs.

connector.set_flash_duration(serial_number, duration=120)

If you do NOT have an Omni use set_camera_settings.

Getting a z-stack

This function will return a list of pillow images. Each image will be at a different focus level.

This code will create a z-stack of 6 images. The focuses of these images will be [0.5, 0.6, 0.7, 0.8, 0.9, 1.0]

You need to give the serial number of the device you want to target.

list_of_imgs = connector.get_z_stack(serial_number, num_img = 6, start_focus = 0.5, stop_focus = 1)

Change stage (Omni only)

To change the position of the Omni stage (camera, led, arm, ect.) give the new position in mm.

• Position x in mm
• Position y in mm
• Time in seconds it can wait till it arrives (default 60)

connector.move_stage(serial_number, 100, 100, 60)

After this the normal process for obtaining and changing cameras applies.

Get stage position (Omni only)

If you need to know where the stage is, use get_position. This also works if the Omni moved without a move_stage command (e.g. when it goes to sleep).

print(connector.get_position(serial_number))

Changing zoom modes (Lux only)

There are 2 zoom modes: "IN" and "OUT". While zoomed in the resolution is higher but the ROI is smaller, zoomed out has a higher ROI but a lower resolution. Since zooming in comprises digital zoom, the image will not show more sample details.

Changing this setting will change it for every image or z-stack taken afterwards.

You need to give the serial number of the device you want to target.

connector.set_zoom(serial_number, "IN")
img1 = connector.get_image(serial_number) # Image is zoomed in
connector.set_zoom(serial_number, "OUT")
img2 = connector.get_image(serial_number) # Image is zoomed out

Live view

The live view of the device is hosted at http://localhost:3333/cytosmartservice/live?serialNumber=##########. This image can only been seen if the live view is turned on (by default the live view is turned on).

You need to give the serial number of the device you want to target at the place of the #-symbols.

connector.set_liveview(serial_number, False) # Led of device turns off till you take a picture
connector.set_liveview(serial_number, True) # In the browser you can see the image being updated
connector.open_liveview(serial_number) # Opens liveview in the default browser

Developers

Developers of the CytoSmartOpenAPI please look at the developers readme

Credits

• Tom Nijhof
• Nora
• Tessa
• Count Nicolai Vondracek, Señior sunshine manager

History

1.3.0 (2022-08-25)

• Add open_liveview
• Add do_autofocus
• Add listener to autofocus change
• Bugfix: set_focus. Make sure camera_setting is completed before setting the focus

1.2.0 (2022-06-27)

• Update server to support Omni
• Add move stage
• Add get position
• Add set flash duration
• Add mandatory turning off of warranty
• Check for connection loss and reconnect is closed
• Check for sleeping device

1.1.1 (2022-06-29)

• Update documentation

1.1.0 (2021-12-03)

• Add change camera settings
• Add set activate camera (brightfield, red-fluo, or green-fluo)
• Change focus: make sure focus always happens in brightfield camera mode Add sleep after changing to focus so camera can move to new position
• Update to new Cytosmart app

1.0.4 (2021-04-15)

• Update Pillow
• Fix installation requirements

1.0.3 (2021-11-22)

• Update requirements of websocket and websocket-client to include version 1
• Check if app is open before starting a new one

1.0.2 (2021-04-15)

• Update pillow
• Update license to ACADEMIC PUBLIC LICENSE

1.0.1 (2021-01-14)

• Added support for LUX3 FL
• Bugfix: toggle liveview when change zoom level

1.0.0 (2020-09-15)

• Added multi lux support
• Changed output from numpy array to pillow
• Require serial number with function to get information
• Add get_temperature

0.1.2 (2020-06-30)

• Rename failing to pinging in print statement

0.1.1 (2020-06-11)

• Update documentation
• Make setup.py fit for PyPI

0.1.0 (2020-05-20)

• Update lux server to latest version

0.0.3 (2020-03-30)

• bugfix: files were not copied into package on linux

0.0.2 (2020-03-24)

• Bugfix: stop waiting for response after sending a message

0.0.1 (2020-03-16)

• Add function: Copy the CytoSmartOpenAPI
• Add function: focus change option
• Add function: z-stack
• Bugfix: add lux app to manifest

0.0.0 (2020-03-13)

Copied basic package a start of CytoSmartOpenAPI

                     ACADEMIC PUBLIC LICENSE
                            version 1.1

                       Copyright (C) 2021


                            Preamble

This license contains the terms and conditions of using CytoSmartOpenAPI in noncommercial settings: at academic institutions for teaching and research use and for personal or educational purposes. You will find that this license provides noncommercial users of CytoSmartOpenAPI with rights that are similar to the well-known GNU General Public License, yet it retains the possibility for CytoSmartOpenAPI authors to financially support the development by selling commercial licenses. In fact, if you intend to use CytoSmartOpenAPI in a "for-profit" environment, where research is conducted to develop or enhance a product, is used in a commercial service offering, or when an entity uses CytoSmartOpenAPI to participate in government-funded, EU-funded, military or similar research projects, then you need to obtain a commercial license. In that case, or if you are unsure, please contact the Author [e-mail] to inquire about commercial licenses.

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We believe that the combination of noncommercial open-source and commercial licensing will be beneficial for the whole user community, because income from commercial licenses will enable faster development and a higher level of software quality, while further enjoying the informal, open communication and collaboration channels of open source development.

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Initially written by Andras Varga (public domain) for OMNeT++ https://omnetpp.org/intro/license, adapted by CytoSMART TECHNOLOGIES BV. The adaptation is licensed under CC0 1.0 (Public Domain Dedication).