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Individually annotate long, error-prone nucleotide sequences into proteins

Project description

Welcome to deciphon ๐Ÿ‘‹

Individually annotate long, error-prone nucleotide sequences into proteins

๐Ÿ  Homepage

โšก๏ธ Requirements

  • Python >= 3.9
  • Pip
  • Podman >= 3.4
  • Homebrew on MacOS (recommended)
  • Pipx for Python package management (recommended)

MacOS

Install Python and Podman:

brew update && brew install python podman pipx

Ensure that your PATH environment variable is all set:

pipx ensurepath

๐Ÿ’ก You might need to close your terminal and reopen it for the changes to take effect.

Ubuntu (and Debian-based distros)

Install Python and Podman:

sudo apt update && \
    sudo apt install python3 python3-pip python3-venv podman --yes && \
    python3 -m pip install --user pipx

Ensure that your PATH environment variable is all set:

python3 -m pipx ensurepath

๐Ÿ’ก You might need to close your terminal and reopen it for the changes to take effect.

Install

pipx install deciphon

Usage

 Usage: deci [OPTIONS] COMMAND [ARGS]...

โ•ญโ”€ Options โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ•ฎ
โ”‚ --version                                                                    โ”‚
โ”‚ --help             Show this message and exit.                               โ”‚
โ•ฐโ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ•ฏ
โ•ญโ”€ Commands โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ•ฎ
โ”‚ press        Press HMM ASCII file into a Deciphon database one.              โ”‚
โ”‚ scan         Annotate nucleotide sequences into proteins a protein database. โ”‚
โ•ฐโ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ”€โ•ฏ

Example

Download the minifam.hmm protein database:

curl -O https://raw.githubusercontent.com/EBI-Metagenomics/deciphon/main/cli/tests/files/minifam.hmm

Download the consensus.fna file of sequences:

curl -O https://raw.githubusercontent.com/EBI-Metagenomics/deciphon/main/cli/tests/files/sequences.fna

Press it (using The Standard Code):

deci press minifam.hmm 1

Scan it:

deci scan minifam.hmm sequences.fna

Show it:

deci see sequences.dcs

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