python script to demultiplex illumina reads tagged with the leeselab tagging scheme
Project description
demultiplexer
python script to demultiplex illumina reads tagged with the leeselab tagging scheme
Installation and startup
To install:
pip install demultiplexer
To run open the terminal and type:
demultiplexer
Tutorial
To run the program download the tutorial data from the repository.
Load the files into demultiplexer.
Load the primerset containing the information for the dataset.
New primersets can be costumized for your needs with the create primerset button.
Create a new tagging scheme with the loaded files and select all combinations used in your dataset.
Fill in the samples in the tagging scheme.
Hit the demultiplex button. If needed the tags can be removed from the sequences.
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