denoiseg 0.3.1

DenoiSeg

DenoiSeg: Joint Denoising and Segmentation

Tim-Oliver Buchholz^*,1,2, Mangal Prakash^*,1,2,, Alexander Krull^1,2,3, and Florian Jug^{1,2,^}

¹ Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany
² Center for Systems Biology, Dresden, Germany
³ Max Planck Institute for Physics of Complex Systems, Dresden, Germany
^{^} [e-mail]
^* Equal contribution (alphabetical order).

Microscopy image analysis often requires the segmentation of objects, but training data for this task is typically scarce and hard to obtain. Here we propose DenoiSeg, a new method that can be trained end-to-end on only a few annotated ground truth segmentations. We achieve this by extending Noise2Void, a self-supervised denoising scheme that can be trained on noisy images alone, to also predict dense 3-class segmentations. The reason for the success of our method is that segmentation can profit from denoising, especially when performed jointly within the same network. The network becomes a denoising expert by seeing all available raw data, while co-learning to segment, even if only a few segmentation labels are available. This hypothesis is additionally fueled by our observation that the best segmentation results on high quality (very low noise) raw data are obtained when moderate amounts of synthetic noise are added. This renders the denoising-task non-trivial and unleashes the desired co-learning effect. We believe that DenoiSeg offers a viable way to circumvent the tremendous hunger for high quality training data and effectively enables few-shot learning of dense segmentations.

Paper: https://arxiv.org/abs/2005.02987

Installation

If you start from scratch...

We recommend using miniconda. If you do not yet have a strong opinion, just use it too!

After installing Miniconda, create a conda environment:

conda create -n 'denoiseg' python=3.9
conda activate denoiseg

Install TensorFlow

The best way to install TensorFLow is to follow the Tensorflow guidelines.

Note that, after installing TensorFlow, running the following commands in your environment will allow you to use the GPU without having to each time run an export command (refer to the Tensorflow step by step):

mkdir -p $CONDA_PREFIX/etc/conda/activate.d
echo 'export LD_LIBRARY_PATH=$LD_LIBRARY_PATH:$CONDA_PREFIX/lib/' > $CONDA_PREFIX/etc/conda/activate.d/env_vars.sh

Option 1: PIP (current stable release)

$ pip install denoiseg

Option 2: Git-Clone and install from sources (current master-branch version)

This option is ideal if you want to edit the code. Clone the repository:

$ git clone https://github.com/juglab/denoiseg.git

Change into its directory and install it:

$ cd denoiseg
$ pip install -e .

You are now ready to run DenoiSeg.

How to use it?

Have a look at our jupyter notebook:

• Example: DSB2018
• Example: Fly Wing
• Example: Mouse Nuclei

How to cite:

@inproceedings{BuchholzPrakash2020DenoiSeg,
  title={DenoiSeg: Joint Denoising and Segmentation},
  author={Tim-Oliver Buchholz and Mangal Prakash and Alexander Krull and Florian Jug},
  year={2020}
}

Reproducibility

The current release and master is a refactored version of the code used for the paper. This refactored version produces the same number as reported in the paper, but if you wish to use the exact code used in the paper, please continue here.

Further results (qualitative and quantitative) can be found on the wiki.