DiMA - Diversity Motif Analyser
Table of Contents
- What is DiMA?
- Publications
- Installation
- Basic Usage
- Advance Usage
- Command-Line Arguments
- Module Parameters
What is DiMA?
Protein sequence diversity is one of the major challenges in the design of diagnostic, prophylactic and therapeutic interventions against viruses. DiMA is a tool designed to facilitate the dissection of protein sequence diversity dynamics for viruses. DiMA provides a quantitative measure of sequence diversity by use of Shannon’s entropy, applied via a user-defined k-mer sliding window. Further, the entropy value is corrected for sample size bias by applying a statistical adjustment. Additionally, DiMA further interrogates the diversity by dissecting the entropy value at each k-mer position to various diversity motifs. The distinct k-mer sequences at each position are classified into the following motifs based on their incidence.
- Index: The predominant sequence.
- Major: The sequence with the second highest incidence after the Index.
- Minor: Kmers with incidence in between major and unique motifs
- Unique: Kmers which are only seen once in a particular kmer position.
Moreover, the description line of the sequences in the alignment can be formatted for inclusion of meta-data that can be tagged to the diversity motifs. DiMA enables comparative diversity dynamics analysis, within and between proteins of a virus species, and proteomes of different viral species.
Publications
Installation
pip install dima-cli
Basic Usage
Shell Command
dima-cli -i aligned_sequences.afa -o results.json
Python
from dima import Dima
results = Dima(sequences="aligned_sequences.afa").run()
Results
Click to view basic results
{
"sequence_count": 5,
"support_threshold": 30,
"low_support_count": 20,
"query_name": "Unknown Query",
"kmer_length": 9,
"average_entropy": 0.06854034285524647,
"highest_entropy": {
"position": 186,
"entropy": 1.3921472236645345
},
"results": [
{
"position": 1,
"low_support": "LS",
"entropy": 0.7219280948873623,
"support": 5,
"distinct_variants_count": 1,
"distinct_variants_incidence": 100.0,
"total_variants_incidence": 20.0,
"diversity_motifs": [
{
"sequence": "MSASKEIKS",
"count": 4,
"incidence": 80.0,
"motif_short": "I",
"motif_long": "Index",
"metadata": null
},
{
"sequence": "SAGVYMGNL",
"count": 1,
"incidence": 20.0,
"motif_short": "U",
"motif_long": "Unique",
"metadata": null
}
]
},
{
"position": 2,
"low_support": "LS",
"entropy": 0.7219280948873623,
"support": 5,
"distinct_variants_count": 1,
"distinct_variants_incidence": 100.0,
"total_variants_incidence": 20.0,
"diversity_motifs": [
{
"sequence": "AGVYMGNLS",
"count": 1,
"incidence": 20.0,
"motif_short": "U",
"motif_long": "Unique",
"metadata": null
},
{
"sequence": "SASKEIKSF",
"count": 4,
"incidence": 80.0,
"motif_short": "I",
"motif_long": "Index",
"metadata": null
}
]
},
{
"position": 3,
"low_support": "LS",
"entropy": 0.7219280948873623,
"support": 5,
"distinct_variants_count": 1,
"distinct_variants_incidence": 100.0,
"total_variants_incidence": 20.0,
"diversity_motifs": [
{
"sequence": "GVYMGNLSS",
"count": 1,
"incidence": 20.0,
"motif_short": "U",
"motif_long": "Unique",
"metadata": null
},
{
"sequence": "ASKEIKSFL",
"count": 4,
"incidence": 80.0,
"motif_short": "I",
"motif_long": "Index",
"metadata": null
}
]
},
{
"position": 4,
"low_support": "LS",
"entropy": 0.7219280948873623,
"support": 5,
"distinct_variants_count": 1,
"distinct_variants_incidence": 100.0,
"total_variants_incidence": 20.0,
"diversity_motifs": [
{
"sequence": "VYMGNLSSQ",
"count": 1,
"incidence": 20.0,
"motif_short": "U",
"motif_long": "Unique",
"metadata": null
},
{
"sequence": "SKEIKSFLW",
"count": 4,
"incidence": 80.0,
"motif_short": "I",
"motif_long": "Index",
"metadata": null
}
]
},
{
"position": 5,
"low_support": "LS",
"entropy": 0.7219280948873623,
"support": 5,
"distinct_variants_count": 1,
"distinct_variants_incidence": 100.0,
"total_variants_incidence": 20.0,
"diversity_motifs": [
{
"sequence": "KEIKSFLWT",
"count": 4,
"incidence": 80.0,
"motif_short": "I",
"motif_long": "Index",
"metadata": null
},
{
"sequence": "YMGNLSSQQ",
"count": 1,
"incidence": 20.0,
"motif_short": "U",
"motif_long": "Unique",
"metadata": null
}
]
},
{
"position": 6,
"low_support": "LS",
"entropy": 0.7219280948873623,
"support": 5,
"distinct_variants_count": 1,
"distinct_variants_incidence": 100.0,
"total_variants_incidence": 20.0,
"diversity_motifs": [
{
"sequence": "MGNLSSQQL",
"count": 1,
"incidence": 20.0,
"motif_short": "U",
"motif_long": "Unique",
"metadata": null
},
{
"sequence": "EIKSFLWTQ",
"count": 4,
"incidence": 80.0,
"motif_short": "I",
"motif_long": "Index",
"metadata": null
}
]
},
{
"position": 7,
"low_support": "LS",
"entropy": 0.7219280948873623,
"support": 5,
"distinct_variants_count": 1,
"distinct_variants_incidence": 100.0,
"total_variants_incidence": 20.0,
"diversity_motifs": [
{
"sequence": "IKSFLWTQS",
"count": 4,
"incidence": 80.0,
"motif_short": "I",
"motif_long": "Index",
"metadata": null
},
{
"sequence": "GNLSSQQLD",
"count": 1,
"incidence": 20.0,
"motif_short": "U",
"motif_long": "Unique",
"metadata": null
}
]
},
{
"position": 8,
"low_support": "LS",
"entropy": 0.7219280948873623,
"support": 5,
"distinct_variants_count": 1,
"distinct_variants_incidence": 100.0,
"total_variants_incidence": 20.0,
"diversity_motifs": [
{
"sequence": "KSFLWTQSL",
"count": 4,
"incidence": 80.0,
"motif_short": "I",
"motif_long": "Index",
"metadata": null
},
{
"sequence": "NLSSQQLDQ",
"count": 1,
"incidence": 20.0,
"motif_short": "U",
"motif_long": "Unique",
"metadata": null
}
]
},
{
"position": 9,
"low_support": "LS",
"entropy": 0.7219280948873623,
"support": 5,
"distinct_variants_count": 1,
"distinct_variants_incidence": 100.0,
"total_variants_incidence": 20.0,
"diversity_motifs": [
{
"sequence": "SFLWTQSLR",
"count": 4,
"incidence": 80.0,
"motif_short": "I",
"motif_long": "Index",
"metadata": null
},
{
"sequence": "LSSQQLDQR",
"count": 1,
"incidence": 20.0,
"motif_short": "U",
"motif_long": "Unique",
"metadata": null
}
]
},
{
"position": 10,
"low_support": "LS",
"entropy": 0.7219280948873623,
"support": 5,
"distinct_variants_count": 1,
"distinct_variants_incidence": 100.0,
"total_variants_incidence": 20.0,
"diversity_motifs": [
{
"sequence": "SSQQLDQRR",
"count": 1,
"incidence": 20.0,
"motif_short": "U",
"motif_long": "Unique",
"metadata": null
},
{
"sequence": "FLWTQSLRR",
"count": 4,
"incidence": 80.0,
"motif_short": "I",
"motif_long": "Index",
"metadata": null
}
]
},
{
"position": 11,
"low_support": "LS",
"entropy": 0.7219280948873623,
"support": 5,
"distinct_variants_count": 1,
"distinct_variants_incidence": 100.0,
"total_variants_incidence": 20.0,
"diversity_motifs": [
{
"sequence": "LWTQSLRRE",
"count": 4,
"incidence": 80.0,
"motif_short": "I",
"motif_long": "Index",
"metadata": null
},
{
"sequence": "SQQLDQRRA",
"count": 1,
"incidence": 20.0,
"motif_short": "U",
"motif_long": "Unique",
"metadata": null
}
]
},
{
"position": 12,
"low_support": "LS",
"entropy": 0.7219280948873623,
"support": 5,
"distinct_variants_count": 1,
"distinct_variants_incidence": 100.0,
"total_variants_incidence": 20.0,
"diversity_motifs": [
{
"sequence": "QQLDQRRAL",
"count": 1,
"incidence": 20.0,
"motif_short": "U",
"motif_long": "Unique",
"metadata": null
},
{
"sequence": "WTQSLRREL",
"count": 4,
"incidence": 80.0,
"motif_short": "I",
"motif_long": "Index",
"metadata": null
}
]
},
{
"position": 13,
"low_support": "LS",
"entropy": 0.7219280948873623,
"support": 5,
"distinct_variants_count": 1,
"distinct_variants_incidence": 100.0,
"total_variants_incidence": 20.0,
"diversity_motifs": [
{
"sequence": "TQSLRRELS",
"count": 4,
"incidence": 80.0,
"motif_short": "I",
"motif_long": "Index",
"metadata": null
},
{
"sequence": "QLDQRRALL",
"count": 1,
"incidence": 20.0,
"motif_short": "U",
"motif_long": "Unique",
"metadata": null
}
]
},
{
"position": 14,
"low_support": "LS",
"entropy": 1.3709505944546687,
"support": 5,
"distinct_variants_count": 2,
"distinct_variants_incidence": 100.0,
"total_variants_incidence": 40.0,
"diversity_motifs": [
{
"sequence": "QSLRRELSG",
"count": 3,
"incidence": 60.000004,
"motif_short": "I",
"motif_long": "Index",
"metadata": null
},
{
"sequence": "LDQRRALLS",
"count": 1,
"incidence": 20.0,
"motif_short": "U",
"motif_long": "Unique",
"metadata": null
},
{
"sequence": "QSLRRELSS",
"count": 1,
"incidence": 20.0,
"motif_short": "U",
"motif_long": "Unique",
"metadata": null
}
]
},
{
"position": 15,
"low_support": "LS",
"entropy": 1.3709505944546687,
"support": 5,
"distinct_variants_count": 2,
"distinct_variants_incidence": 100.0,
"total_variants_incidence": 40.0,
"diversity_motifs": [
{
"sequence": "DQRRALLSM",
"count": 1,
"incidence": 20.0,
"motif_short": "U",
"motif_long": "Unique",
"metadata": null
},
{
"sequence": "SLRRELSGY",
"count": 3,
"incidence": 60.000004,
"motif_short": "I",
"motif_long": "Index",
"metadata": null
},
{
"sequence": "SLRRELSSY",
"count": 1,
"incidence": 20.0,
"motif_short": "U",
"motif_long": "Unique",
"metadata": null
}
]
},
{
"position": 16,
"low_support": "LS",
"entropy": 1.3709505944546687,
"support": 5,
"distinct_variants_count": 2,
"distinct_variants_incidence": 100.0,
"total_variants_incidence": 40.0,
"diversity_motifs": [
{
"sequence": "QRRALLSMI",
"count": 1,
"incidence": 20.0,
"motif_short": "U",
"motif_long": "Unique",
"metadata": null
},
{
"sequence": "LRRELSSYC",
"count": 1,
"incidence": 20.0,
"motif_short": "U",
"motif_long": "Unique",
"metadata": null
},
{
"sequence": "LRRELSGYC",
"count": 3,
"incidence": 60.000004,
"motif_short": "I",
"motif_long": "Index",
"metadata": null
}
]
},
{
"position": 17,
"low_support": "LS",
"entropy": 1.3709505944546687,
"support": 5,
"distinct_variants_count": 2,
"distinct_variants_incidence": 100.0,
"total_variants_incidence": 40.0,
"diversity_motifs": [
{
"sequence": "RRELSGYCS",
"count": 3,
"incidence": 60.000004,
"motif_short": "I",
"motif_long": "Index",
"metadata": null
},
{
"sequence": "RRALLSMIG",
"count": 1,
"incidence": 20.0,
"motif_short": "U",
"motif_long": "Unique",
"metadata": null
},
{
"sequence": "RRELSSYCS",
"count": 1,
"incidence": 20.0,
"motif_short": "U",
"motif_long": "Unique",
"metadata": null
}
]
},
{
"position": 18,
"low_support": "LS",
"entropy": 1.3709505944546687,
"support": 5,
"distinct_variants_count": 2,
"distinct_variants_incidence": 100.0,
"total_variants_incidence": 40.0,
"diversity_motifs": [
{
"sequence": "RELSGYCSN",
"count": 3,
"incidence": 60.000004,
"motif_short": "I",
"motif_long": "Index",
"metadata": null
},
{
"sequence": "RALLSMIGM",
"count": 1,
"incidence": 20.0,
"motif_short": "U",
"motif_long": "Unique",
"metadata": null
},
{
"sequence": "RELSSYCSN",
"count": 1,
"incidence": 20.0,
"motif_short": "U",
"motif_long": "Unique",
"metadata": null
}
]
},
{
"position": 19,
"low_support": "LS",
"entropy": 1.3709505944546687,
"support": 5,
"distinct_variants_count": 2,
"distinct_variants_incidence": 100.0,
"total_variants_incidence": 40.0,
"diversity_motifs": [
{
"sequence": "ALLSMIGMS",
"count": 1,
"incidence": 20.0,
"motif_short": "U",
"motif_long": "Unique",
"metadata": null
},
{
"sequence": "ELSSYCSNI",
"count": 1,
"incidence": 20.0,
"motif_short": "U",
"motif_long": "Unique",
"metadata": null
},
{
"sequence": "ELSGYCSNI",
"count": 3,
"incidence": 60.000004,
"motif_short": "I",
"motif_long": "Index",
"metadata": null
}
]
},
{
"position": 20,
"low_support": "LS",
"entropy": 1.3709505944546687,
"support": 5,
"distinct_variants_count": 2,
"distinct_variants_incidence": 100.0,
"total_variants_incidence": 40.0,
"diversity_motifs": [
{
"sequence": "LSGYCSNIK",
"count": 3,
"incidence": 60.000004,
"motif_short": "I",
"motif_long": "Index",
"metadata": null
},
{
"sequence": "LLSMIGMSG",
"count": 1,
"incidence": 20.0,
"motif_short": "U",
"motif_long": "Unique",
"metadata": null
},
{
"sequence": "LSSYCSNIK",
"count": 1,
"incidence": 20.0,
"motif_short": "U",
"motif_long": "Unique",
"metadata": null
}
]
}
]
}
Advance Usage
Shell Command
dima-cli -i aligned_sequences.afa -o results.json -f "accession|strain|country|date"
Python
from dima import Dima
results = Dima(sequences="aligned_sequences.afa", header_format="accession|strain|country|date").run()
Results
Click to view advanced results
{
"sequence_count": 5,
"support_threshold": 30,
"low_support_count": 20,
"query_name": "Unknown Query",
"kmer_length": 9,
"average_entropy": 0.06854034285524647,
"highest_entropy": {
"position": 186,
"entropy": 1.3921472236645345
},
"results": [
{
"position": 1,
"low_support": "LS",
"entropy": 0.7219280948873623,
"support": 5,
"distinct_variants_count": 1,
"distinct_variants_incidence": 100.0,
"total_variants_incidence": 20.0,
"diversity_motifs": [
{
"sequence": "MSASKEIKS",
"count": 4,
"incidence": 80.0,
"motif_short": "I",
"motif_long": "Index",
"metadata": {
"Accession": {
"AYD75365.1": 1,
"QEP52131.1": 1,
"AYD75321.1": 1,
"AYD75325.1": 1
},
"Country": {
"Sierra Leone": 4
},
"Species": {
"Homo sapiens": 3,
"Unknown": 1
},
"Year": {
"1977": 1,
"2012": 1,
"1980": 1,
"1979": 1
}
}
},
{
"sequence": "SAGVYMGNL",
"count": 1,
"incidence": 20.0,
"motif_short": "U",
"motif_long": "Unique",
"metadata": {
"Accession": {
"AYD75329.1": 1
},
"Country": {
"Sierra Leone": 1
},
"Year": {
"1975": 1
},
"Species": {
"Homo sapiens": 1
}
}
}
]
},
{
"position": 2,
"low_support": "LS",
"entropy": 0.7219280948873623,
"support": 5,
"distinct_variants_count": 1,
"distinct_variants_incidence": 100.0,
"total_variants_incidence": 20.0,
"diversity_motifs": [
{
"sequence": "SASKEIKSF",
"count": 4,
"incidence": 80.0,
"motif_short": "I",
"motif_long": "Index",
"metadata": {
"Species": {
"Homo sapiens": 3,
"Unknown": 1
},
"Year": {
"1977": 1,
"1980": 1,
"1979": 1,
"2012": 1
},
"Country": {
"Sierra Leone": 4
},
"Accession": {
"AYD75325.1": 1,
"QEP52131.1": 1,
"AYD75365.1": 1,
"AYD75321.1": 1
}
}
},
{
"sequence": "AGVYMGNLS",
"count": 1,
"incidence": 20.0,
"motif_short": "U",
"motif_long": "Unique",
"metadata": {
"Species": {
"Homo sapiens": 1
},
"Year": {
"1975": 1
},
"Accession": {
"AYD75329.1": 1
},
"Country": {
"Sierra Leone": 1
}
}
}
]
},
{
"position": 3,
"low_support": "LS",
"entropy": 0.7219280948873623,
"support": 5,
"distinct_variants_count": 1,
"distinct_variants_incidence": 100.0,
"total_variants_incidence": 20.0,
"diversity_motifs": [
{
"sequence": "ASKEIKSFL",
"count": 4,
"incidence": 80.0,
"motif_short": "I",
"motif_long": "Index",
"metadata": {
"Year": {
"1980": 1,
"1977": 1,
"1979": 1,
"2012": 1
},
"Accession": {
"AYD75321.1": 1,
"AYD75365.1": 1,
"QEP52131.1": 1,
"AYD75325.1": 1
},
"Country": {
"Sierra Leone": 4
},
"Species": {
"Unknown": 1,
"Homo sapiens": 3
}
}
},
{
"sequence": "GVYMGNLSS",
"count": 1,
"incidence": 20.0,
"motif_short": "U",
"motif_long": "Unique",
"metadata": {
"Accession": {
"AYD75329.1": 1
},
"Species": {
"Homo sapiens": 1
},
"Country": {
"Sierra Leone": 1
},
"Year": {
"1975": 1
}
}
}
]
},
{
"position": 4,
"low_support": "LS",
"entropy": 0.7219280948873623,
"support": 5,
"distinct_variants_count": 1,
"distinct_variants_incidence": 100.0,
"total_variants_incidence": 20.0,
"diversity_motifs": [
{
"sequence": "SKEIKSFLW",
"count": 4,
"incidence": 80.0,
"motif_short": "I",
"motif_long": "Index",
"metadata": {
"Accession": {
"AYD75325.1": 1,
"AYD75365.1": 1,
"QEP52131.1": 1,
"AYD75321.1": 1
},
"Country": {
"Sierra Leone": 4
},
"Species": {
"Unknown": 1,
"Homo sapiens": 3
},
"Year": {
"2012": 1,
"1979": 1,
"1980": 1,
"1977": 1
}
}
},
{
"sequence": "VYMGNLSSQ",
"count": 1,
"incidence": 20.0,
"motif_short": "U",
"motif_long": "Unique",
"metadata": {
"Accession": {
"AYD75329.1": 1
},
"Year": {
"1975": 1
},
"Species": {
"Homo sapiens": 1
},
"Country": {
"Sierra Leone": 1
}
}
}
]
},
{
"position": 5,
"low_support": "LS",
"entropy": 0.7219280948873623,
"support": 5,
"distinct_variants_count": 1,
"distinct_variants_incidence": 100.0,
"total_variants_incidence": 20.0,
"diversity_motifs": [
{
"sequence": "KEIKSFLWT",
"count": 4,
"incidence": 80.0,
"motif_short": "I",
"motif_long": "Index",
"metadata": {
"Accession": {
"QEP52131.1": 1,
"AYD75325.1": 1,
"AYD75321.1": 1,
"AYD75365.1": 1
},
"Year": {
"1979": 1,
"1980": 1,
"1977": 1,
"2012": 1
},
"Country": {
"Sierra Leone": 4
},
"Species": {
"Homo sapiens": 3,
"Unknown": 1
}
}
},
{
"sequence": "YMGNLSSQQ",
"count": 1,
"incidence": 20.0,
"motif_short": "U",
"motif_long": "Unique",
"metadata": {
"Country": {
"Sierra Leone": 1
},
"Year": {
"1975": 1
},
"Species": {
"Homo sapiens": 1
},
"Accession": {
"AYD75329.1": 1
}
}
}
]
},
{
"position": 6,
"low_support": "LS",
"entropy": 0.7219280948873623,
"support": 5,
"distinct_variants_count": 1,
"distinct_variants_incidence": 100.0,
"total_variants_incidence": 20.0,
"diversity_motifs": [
{
"sequence": "MGNLSSQQL",
"count": 1,
"incidence": 20.0,
"motif_short": "U",
"motif_long": "Unique",
"metadata": {
"Accession": {
"AYD75329.1": 1
},
"Country": {
"Sierra Leone": 1
},
"Species": {
"Homo sapiens": 1
},
"Year": {
"1975": 1
}
}
},
{
"sequence": "EIKSFLWTQ",
"count": 4,
"incidence": 80.0,
"motif_short": "I",
"motif_long": "Index",
"metadata": {
"Accession": {
"AYD75321.1": 1,
"QEP52131.1": 1,
"AYD75365.1": 1,
"AYD75325.1": 1
},
"Species": {
"Unknown": 1,
"Homo sapiens": 3
},
"Country": {
"Sierra Leone": 4
},
"Year": {
"1977": 1,
"1980": 1,
"2012": 1,
"1979": 1
}
}
}
]
},
{
"position": 7,
"low_support": "LS",
"entropy": 0.7219280948873623,
"support": 5,
"distinct_variants_count": 1,
"distinct_variants_incidence": 100.0,
"total_variants_incidence": 20.0,
"diversity_motifs": [
{
"sequence": "GNLSSQQLD",
"count": 1,
"incidence": 20.0,
"motif_short": "U",
"motif_long": "Unique",
"metadata": {
"Country": {
"Sierra Leone": 1
},
"Species": {
"Homo sapiens": 1
},
"Accession": {
"AYD75329.1": 1
},
"Year": {
"1975": 1
}
}
},
{
"sequence": "IKSFLWTQS",
"count": 4,
"incidence": 80.0,
"motif_short": "I",
"motif_long": "Index",
"metadata": {
"Accession": {
"AYD75365.1": 1,
"AYD75325.1": 1,
"QEP52131.1": 1,
"AYD75321.1": 1
},
"Year": {
"1979": 1,
"1980": 1,
"1977": 1,
"2012": 1
},
"Species": {
"Unknown": 1,
"Homo sapiens": 3
},
"Country": {
"Sierra Leone": 4
}
}
}
]
},
{
"position": 8,
"low_support": "LS",
"entropy": 0.7219280948873623,
"support": 5,
"distinct_variants_count": 1,
"distinct_variants_incidence": 100.0,
"total_variants_incidence": 20.0,
"diversity_motifs": [
{
"sequence": "NLSSQQLDQ",
"count": 1,
"incidence": 20.0,
"motif_short": "U",
"motif_long": "Unique",
"metadata": {
"Country": {
"Sierra Leone": 1
},
"Year": {
"1975": 1
},
"Species": {
"Homo sapiens": 1
},
"Accession": {
"AYD75329.1": 1
}
}
},
{
"sequence": "KSFLWTQSL",
"count": 4,
"incidence": 80.0,
"motif_short": "I",
"motif_long": "Index",
"metadata": {
"Country": {
"Sierra Leone": 4
},
"Year": {
"1979": 1,
"2012": 1,
"1977": 1,
"1980": 1
},
"Species": {
"Unknown": 1,
"Homo sapiens": 3
},
"Accession": {
"AYD75325.1": 1,
"AYD75365.1": 1,
"AYD75321.1": 1,
"QEP52131.1": 1
}
}
}
]
},
{
"position": 9,
"low_support": "LS",
"entropy": 0.7219280948873623,
"support": 5,
"distinct_variants_count": 1,
"distinct_variants_incidence": 100.0,
"total_variants_incidence": 20.0,
"diversity_motifs": [
{
"sequence": "SFLWTQSLR",
"count": 4,
"incidence": 80.0,
"motif_short": "I",
"motif_long": "Index",
"metadata": {
"Year": {
"2012": 1,
"1979": 1,
"1980": 1,
"1977": 1
},
"Accession": {
"QEP52131.1": 1,
"AYD75325.1": 1,
"AYD75365.1": 1,
"AYD75321.1": 1
},
"Country": {
"Sierra Leone": 4
},
"Species": {
"Unknown": 1,
"Homo sapiens": 3
}
}
},
{
"sequence": "LSSQQLDQR",
"count": 1,
"incidence": 20.0,
"motif_short": "U",
"motif_long": "Unique",
"metadata": {
"Species": {
"Homo sapiens": 1
},
"Accession": {
"AYD75329.1": 1
},
"Year": {
"1975": 1
},
"Country": {
"Sierra Leone": 1
}
}
}
]
},
{
"position": 10,
"low_support": "LS",
"entropy": 0.7219280948873623,
"support": 5,
"distinct_variants_count": 1,
"distinct_variants_incidence": 100.0,
"total_variants_incidence": 20.0,
"diversity_motifs": [
{
"sequence": "FLWTQSLRR",
"count": 4,
"incidence": 80.0,
"motif_short": "I",
"motif_long": "Index",
"metadata": {
"Year": {
"1977": 1,
"2012": 1,
"1980": 1,
"1979": 1
},
"Accession": {
"AYD75321.1": 1,
"AYD75325.1": 1,
"AYD75365.1": 1,
"QEP52131.1": 1
},
"Species": {
"Unknown": 1,
"Homo sapiens": 3
},
"Country": {
"Sierra Leone": 4
}
}
},
{
"sequence": "SSQQLDQRR",
"count": 1,
"incidence": 20.0,
"motif_short": "U",
"motif_long": "Unique",
"metadata": {
"Species": {
"Homo sapiens": 1
},
"Year": {
"1975": 1
},
"Accession": {
"AYD75329.1": 1
},
"Country": {
"Sierra Leone": 1
}
}
}
]
},
{
"position": 11,
"low_support": "LS",
"entropy": 0.7219280948873623,
"support": 5,
"distinct_variants_count": 1,
"distinct_variants_incidence": 100.0,
"total_variants_incidence": 20.0,
"diversity_motifs": [
{
"sequence": "LWTQSLRRE",
"count": 4,
"incidence": 80.0,
"motif_short": "I",
"motif_long": "Index",
"metadata": {
"Country": {
"Sierra Leone": 4
},
"Accession": {
"QEP52131.1": 1,
"AYD75365.1": 1,
"AYD75325.1": 1,
"AYD75321.1": 1
},
"Species": {
"Homo sapiens": 3,
"Unknown": 1
},
"Year": {
"1979": 1,
"1980": 1,
"2012": 1,
"1977": 1
}
}
},
{
"sequence": "SQQLDQRRA",
"count": 1,
"incidence": 20.0,
"motif_short": "U",
"motif_long": "Unique",
"metadata": {
"Accession": {
"AYD75329.1": 1
},
"Species": {
"Homo sapiens": 1
},
"Country": {
"Sierra Leone": 1
},
"Year": {
"1975": 1
}
}
}
]
},
{
"position": 12,
"low_support": "LS",
"entropy": 0.7219280948873623,
"support": 5,
"distinct_variants_count": 1,
"distinct_variants_incidence": 100.0,
"total_variants_incidence": 20.0,
"diversity_motifs": [
{
"sequence": "QQLDQRRAL",
"count": 1,
"incidence": 20.0,
"motif_short": "U",
"motif_long": "Unique",
"metadata": {
"Country": {
"Sierra Leone": 1
},
"Species": {
"Homo sapiens": 1
},
"Year": {
"1975": 1
},
"Accession": {
"AYD75329.1": 1
}
}
},
{
"sequence": "WTQSLRREL",
"count": 4,
"incidence": 80.0,
"motif_short": "I",
"motif_long": "Index",
"metadata": {
"Country": {
"Sierra Leone": 4
},
"Year": {
"1980": 1,
"2012": 1,
"1979": 1,
"1977": 1
},
"Accession": {
"QEP52131.1": 1,
"AYD75321.1": 1,
"AYD75325.1": 1,
"AYD75365.1": 1
},
"Species": {
"Homo sapiens": 3,
"Unknown": 1
}
}
}
]
},
{
"position": 13,
"low_support": "LS",
"entropy": 0.7219280948873623,
"support": 5,
"distinct_variants_count": 1,
"distinct_variants_incidence": 100.0,
"total_variants_incidence": 20.0,
"diversity_motifs": [
{
"sequence": "TQSLRRELS",
"count": 4,
"incidence": 80.0,
"motif_short": "I",
"motif_long": "Index",
"metadata": {
"Accession": {
"AYD75365.1": 1,
"AYD75321.1": 1,
"QEP52131.1": 1,
"AYD75325.1": 1
},
"Country": {
"Sierra Leone": 4
},
"Year": {
"1977": 1,
"1979": 1,
"2012": 1,
"1980": 1
},
"Species": {
"Unknown": 1,
"Homo sapiens": 3
}
}
},
{
"sequence": "QLDQRRALL",
"count": 1,
"incidence": 20.0,
"motif_short": "U",
"motif_long": "Unique",
"metadata": {
"Year": {
"1975": 1
},
"Accession": {
"AYD75329.1": 1
},
"Country": {
"Sierra Leone": 1
},
"Species": {
"Homo sapiens": 1
}
}
}
]
},
{
"position": 14,
"low_support": "LS",
"entropy": 1.3709505944546687,
"support": 5,
"distinct_variants_count": 2,
"distinct_variants_incidence": 100.0,
"total_variants_incidence": 40.0,
"diversity_motifs": [
{
"sequence": "QSLRRELSG",
"count": 3,
"incidence": 60.000004,
"motif_short": "I",
"motif_long": "Index",
"metadata": {
"Country": {
"Sierra Leone": 3
},
"Accession": {
"AYD75325.1": 1,
"AYD75321.1": 1,
"AYD75365.1": 1
},
"Species": {
"Homo sapiens": 2,
"Unknown": 1
},
"Year": {
"1979": 1,
"1980": 1,
"1977": 1
}
}
},
{
"sequence": "QSLRRELSS",
"count": 1,
"incidence": 20.0,
"motif_short": "U",
"motif_long": "Unique",
"metadata": {
"Country": {
"Sierra Leone": 1
},
"Year": {
"2012": 1
},
"Species": {
"Homo sapiens": 1
},
"Accession": {
"QEP52131.1": 1
}
}
},
{
"sequence": "LDQRRALLS",
"count": 1,
"incidence": 20.0,
"motif_short": "U",
"motif_long": "Unique",
"metadata": {
"Country": {
"Sierra Leone": 1
},
"Species": {
"Homo sapiens": 1
},
"Accession": {
"AYD75329.1": 1
},
"Year": {
"1975": 1
}
}
}
]
},
{
"position": 15,
"low_support": "LS",
"entropy": 1.3709505944546687,
"support": 5,
"distinct_variants_count": 2,
"distinct_variants_incidence": 100.0,
"total_variants_incidence": 40.0,
"diversity_motifs": [
{
"sequence": "DQRRALLSM",
"count": 1,
"incidence": 20.0,
"motif_short": "U",
"motif_long": "Unique",
"metadata": {
"Country": {
"Sierra Leone": 1
},
"Accession": {
"AYD75329.1": 1
},
"Species": {
"Homo sapiens": 1
},
"Year": {
"1975": 1
}
}
},
{
"sequence": "SLRRELSSY",
"count": 1,
"incidence": 20.0,
"motif_short": "U",
"motif_long": "Unique",
"metadata": {
"Accession": {
"QEP52131.1": 1
},
"Country": {
"Sierra Leone": 1
},
"Species": {
"Homo sapiens": 1
},
"Year": {
"2012": 1
}
}
},
{
"sequence": "SLRRELSGY",
"count": 3,
"incidence": 60.000004,
"motif_short": "I",
"motif_long": "Index",
"metadata": {
"Species": {
"Unknown": 1,
"Homo sapiens": 2
},
"Year": {
"1977": 1,
"1980": 1,
"1979": 1
},
"Country": {
"Sierra Leone": 3
},
"Accession": {
"AYD75365.1": 1,
"AYD75325.1": 1,
"AYD75321.1": 1
}
}
}
]
},
{
"position": 16,
"low_support": "LS",
"entropy": 1.3709505944546687,
"support": 5,
"distinct_variants_count": 2,
"distinct_variants_incidence": 100.0,
"total_variants_incidence": 40.0,
"diversity_motifs": [
{
"sequence": "LRRELSSYC",
"count": 1,
"incidence": 20.0,
"motif_short": "U",
"motif_long": "Unique",
"metadata": {
"Country": {
"Sierra Leone": 1
},
"Accession": {
"QEP52131.1": 1
},
"Year": {
"2012": 1
},
"Species": {
"Homo sapiens": 1
}
}
},
{
"sequence": "LRRELSGYC",
"count": 3,
"incidence": 60.000004,
"motif_short": "I",
"motif_long": "Index",
"metadata": {
"Year": {
"1979": 1,
"1977": 1,
"1980": 1
},
"Species": {
"Unknown": 1,
"Homo sapiens": 2
},
"Country": {
"Sierra Leone": 3
},
"Accession": {
"AYD75365.1": 1,
"AYD75321.1": 1,
"AYD75325.1": 1
}
}
},
{
"sequence": "QRRALLSMI",
"count": 1,
"incidence": 20.0,
"motif_short": "U",
"motif_long": "Unique",
"metadata": {
"Species": {
"Homo sapiens": 1
},
"Country": {
"Sierra Leone": 1
},
"Accession": {
"AYD75329.1": 1
},
"Year": {
"1975": 1
}
}
}
]
},
{
"position": 17,
"low_support": "LS",
"entropy": 1.3709505944546687,
"support": 5,
"distinct_variants_count": 2,
"distinct_variants_incidence": 100.0,
"total_variants_incidence": 40.0,
"diversity_motifs": [
{
"sequence": "RRELSSYCS",
"count": 1,
"incidence": 20.0,
"motif_short": "U",
"motif_long": "Unique",
"metadata": {
"Year": {
"2012": 1
},
"Accession": {
"QEP52131.1": 1
},
"Country": {
"Sierra Leone": 1
},
"Species": {
"Homo sapiens": 1
}
}
},
{
"sequence": "RRELSGYCS",
"count": 3,
"incidence": 60.000004,
"motif_short": "I",
"motif_long": "Index",
"metadata": {
"Species": {
"Homo sapiens": 2,
"Unknown": 1
},
"Year": {
"1980": 1,
"1977": 1,
"1979": 1
},
"Accession": {
"AYD75325.1": 1,
"AYD75365.1": 1,
"AYD75321.1": 1
},
"Country": {
"Sierra Leone": 3
}
}
},
{
"sequence": "RRALLSMIG",
"count": 1,
"incidence": 20.0,
"motif_short": "U",
"motif_long": "Unique",
"metadata": {
"Country": {
"Sierra Leone": 1
},
"Species": {
"Homo sapiens": 1
},
"Accession": {
"AYD75329.1": 1
},
"Year": {
"1975": 1
}
}
}
]
},
{
"position": 18,
"low_support": "LS",
"entropy": 1.3709505944546687,
"support": 5,
"distinct_variants_count": 2,
"distinct_variants_incidence": 100.0,
"total_variants_incidence": 40.0,
"diversity_motifs": [
{
"sequence": "RALLSMIGM",
"count": 1,
"incidence": 20.0,
"motif_short": "U",
"motif_long": "Unique",
"metadata": {
"Year": {
"1975": 1
},
"Accession": {
"AYD75329.1": 1
},
"Country": {
"Sierra Leone": 1
},
"Species": {
"Homo sapiens": 1
}
}
},
{
"sequence": "RELSSYCSN",
"count": 1,
"incidence": 20.0,
"motif_short": "U",
"motif_long": "Unique",
"metadata": {
"Species": {
"Homo sapiens": 1
},
"Accession": {
"QEP52131.1": 1
},
"Country": {
"Sierra Leone": 1
},
"Year": {
"2012": 1
}
}
},
{
"sequence": "RELSGYCSN",
"count": 3,
"incidence": 60.000004,
"motif_short": "I",
"motif_long": "Index",
"metadata": {
"Accession": {
"AYD75325.1": 1,
"AYD75365.1": 1,
"AYD75321.1": 1
},
"Species": {
"Homo sapiens": 2,
"Unknown": 1
},
"Year": {
"1980": 1,
"1977": 1,
"1979": 1
},
"Country": {
"Sierra Leone": 3
}
}
}
]
},
{
"position": 19,
"low_support": "LS",
"entropy": 1.3709505944546687,
"support": 5,
"distinct_variants_count": 2,
"distinct_variants_incidence": 100.0,
"total_variants_incidence": 40.0,
"diversity_motifs": [
{
"sequence": "ELSGYCSNI",
"count": 3,
"incidence": 60.000004,
"motif_short": "I",
"motif_long": "Index",
"metadata": {
"Country": {
"Sierra Leone": 3
},
"Species": {
"Homo sapiens": 2,
"Unknown": 1
},
"Accession": {
"AYD75365.1": 1,
"AYD75325.1": 1,
"AYD75321.1": 1
},
"Year": {
"1977": 1,
"1979": 1,
"1980": 1
}
}
},
{
"sequence": "ELSSYCSNI",
"count": 1,
"incidence": 20.0,
"motif_short": "U",
"motif_long": "Unique",
"metadata": {
"Accession": {
"QEP52131.1": 1
},
"Species": {
"Homo sapiens": 1
},
"Country": {
"Sierra Leone": 1
},
"Year": {
"2012": 1
}
}
},
{
"sequence": "ALLSMIGMS",
"count": 1,
"incidence": 20.0,
"motif_short": "U",
"motif_long": "Unique",
"metadata": {
"Country": {
"Sierra Leone": 1
},
"Accession": {
"AYD75329.1": 1
},
"Species": {
"Homo sapiens": 1
},
"Year": {
"1975": 1
}
}
}
]
},
{
"position": 20,
"low_support": "LS",
"entropy": 1.3709505944546687,
"support": 5,
"distinct_variants_count": 2,
"distinct_variants_incidence": 100.0,
"total_variants_incidence": 40.0,
"diversity_motifs": [
{
"sequence": "LLSMIGMSG",
"count": 1,
"incidence": 20.0,
"motif_short": "U",
"motif_long": "Unique",
"metadata": {
"Accession": {
"AYD75329.1": 1
},
"Country": {
"Sierra Leone": 1
},
"Species": {
"Homo sapiens": 1
},
"Year": {
"1975": 1
}
}
},
{
"sequence": "LSGYCSNIK",
"count": 3,
"incidence": 60.000004,
"motif_short": "I",
"motif_long": "Index",
"metadata": {
"Country": {
"Sierra Leone": 3
},
"Year": {
"1979": 1,
"1977": 1,
"1980": 1
},
"Accession": {
"AYD75365.1": 1,
"AYD75321.1": 1,
"AYD75325.1": 1
},
"Species": {
"Unknown": 1,
"Homo sapiens": 2
}
}
},
{
"sequence": "LSSYCSNIK",
"count": 1,
"incidence": 20.0,
"motif_short": "U",
"motif_long": "Unique",
"metadata": {
"Year": {
"2012": 1
},
"Species": {
"Homo sapiens": 1
},
"Accession": {
"QEP52131.1": 1
},
"Country": {
"Sierra Leone": 1
}
}
}
]
}
]
}
Command-Line Arguments
| Argument | Type | Required | Default | Example | Description |
|---|---|---|---|---|---|
| -h | N/A | False | N/A | dima-cli -h |
Prints a summary of all available command-line arguments. |
| -n | String | False | Unknown | dima-cli -i sequences.afa -o results.json -f "accession|strain|country" -n "NA" -n "Unknown" |
Silently fix missing values in the FASTA header with given value. |
| -v | N/A | False | N/A | dima-cli -v |
Prints the version of dima-cli that is currently installed. |
| -q | String | False | Unknown Query | dima-cli -q "Coronavirus Surface Protein" -i sequences.afa -o results.json |
The name of the sample that will appear on the results. |
| -i | String | True | N/A | dima-cli -i sequences.afa -o results.json |
The path to the FASTA Multiple Sequence Alignment file. |
| -o | String | True | N/A | dima-cli -i sequences.afa -o results,json |
The location where the results shall be saved. |
| -l | Integer | False | 9 | dima-cli -i sequences.afa -l 12 -o results.json |
The length of the kmers generated. |
| -f | String | False | N/A | dima-cli -i sequences.afa -f "accession|strain|country" -o results.json |
The format of the FASTA header. Labels where each variant of a kmer position originated from. |
| -s | Integer | False | 30 | dima-cli -i sequences.afa -l 12 -s 40 -o results.json |
The minimum required support for each kmer position. |
| -a | nucleotide/protein | False | protein | dima-cli -i dna_sequences.afa -a nucleotide -o results.json |
The alphabet of the sequences (ie: protein/nucleotide, default: protein) |
| -t | json/xlsx | False | json | dima-cli -i dna_sequences.afa -a nucleotide -o results.json -t xlsx |
The output format (ie: json/xlsx, default: json) |
| -c | String | False | N/A | dima-cli -i dna_sequences.afa -a nucleotide -o results.json -c hcs.json |
Path to save Highly Conserved Sequences (HCS) in JSON format. |
| -e | Float | False | 100 | dima-cli -i dna_sequences.afa -a nucleotide -o results.json -c hcs.json -e 90.5 |
Minimum incidence (%) threshold for HCS concatenation. |
Module Parameters
| Parameter | Type | Required | Default | Description |
|---|---|---|---|---|
| sequences | String/StringIO | True | N/A | The path to a FASTA Multiple Sequence Alignment file (MSA), or a StringIO object containing FASTA MSA. |
| kmer_length | Integer | False | 9 | The length of the kmers generated. |
| header_fillna | String | False | Unknown | Silently fix missing values in the FASTA header with given value (only required when header_format is given). |
| header_format | String | False | N/A | The format of the FASTA header. Labels where each variant of a kmer position originated from. |
| support_threshold | Integer | False | 30 | The minimum required support for each kmer position. |
| query_name | String | False | Unknown Query | The name of the sample that will appear on the results. |
| alphabet | String | False | protein | The alphabet of the sequences (ie: protein/nucleotide, default: protein) |
1 maintainer
Metadata
1 maintainer
Release files for dima-cli 5.0.9
For a detailed explanation of source distributions (sdists) and built distributions (wheels), please see the package formats documentation.
Built distributions (wheels)
Total release size: 6.1 MB
Release files / dima_cli-5.0.9-cp311-none-win_amd64.whl
| Download URL | dima_cli-5.0.9-cp311-none-win_amd64.whl |
|---|---|
| Size | 288.3 kB |
| Tags | CPython 3.11 Windows x86-64 |
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maturin/1.7.1
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Release files / dima_cli-5.0.9-cp311-cp311-manylinux_2_17_x86_64.manylinux2014_x86_64.whl
| Download URL | dima_cli-5.0.9-cp311-cp311-manylinux_2_17_x86_64.manylinux2014_x86_64.whl |
|---|---|
| Size | 446.2 kB |
| Tags | CPython 3.11 Linux glibc 2.17+ x86-64 |
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Release files / dima_cli-5.0.9-cp311-cp311-macosx_10_12_x86_64.macosx_11_0_arm64.macosx_10_12_universal2.whl
| Download URL | dima_cli-5.0.9-cp311-cp311-macosx_10_12_x86_64.macosx_11_0_arm64.macosx_10_12_universal2.whl |
|---|---|
| Size | 779.7 kB |
| Tags | CPython 3.11 macOS 10.12+ universal2 (ARM64, x86-64) macOS 10.12+ x86-64 macOS 11.0+ ARM64 |
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Release files / dima_cli-5.0.9-cp310-none-win_amd64.whl
| Download URL | dima_cli-5.0.9-cp310-none-win_amd64.whl |
|---|---|
| Size | 288.1 kB |
| Tags | CPython 3.10 Windows x86-64 |
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Release files / dima_cli-5.0.9-cp310-cp310-manylinux_2_17_x86_64.manylinux2014_x86_64.whl
| Download URL | dima_cli-5.0.9-cp310-cp310-manylinux_2_17_x86_64.manylinux2014_x86_64.whl |
|---|---|
| Size | 446.6 kB |
| Tags | CPython 3.10 Linux glibc 2.17+ x86-64 |
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Release files / dima_cli-5.0.9-cp310-cp310-macosx_10_12_x86_64.macosx_11_0_arm64.macosx_10_12_universal2.whl
| Download URL | dima_cli-5.0.9-cp310-cp310-macosx_10_12_x86_64.macosx_11_0_arm64.macosx_10_12_universal2.whl |
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| Size | 780.8 kB |
| Tags | CPython 3.10 macOS 10.12+ universal2 (ARM64, x86-64) macOS 10.12+ x86-64 macOS 11.0+ ARM64 |
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Release files / dima_cli-5.0.9-cp39-none-win_amd64.whl
| Download URL | dima_cli-5.0.9-cp39-none-win_amd64.whl |
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| Size | 288.1 kB |
| Tags | CPython 3.9 Windows x86-64 |
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Release files / dima_cli-5.0.9-cp39-cp39-manylinux_2_17_x86_64.manylinux2014_x86_64.whl
| Download URL | dima_cli-5.0.9-cp39-cp39-manylinux_2_17_x86_64.manylinux2014_x86_64.whl |
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| Size | 447.2 kB |
| Tags | CPython 3.9 Linux glibc 2.17+ x86-64 |
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Release files / dima_cli-5.0.9-cp39-cp39-macosx_10_12_x86_64.macosx_11_0_arm64.macosx_10_12_universal2.whl
| Download URL | dima_cli-5.0.9-cp39-cp39-macosx_10_12_x86_64.macosx_11_0_arm64.macosx_10_12_universal2.whl |
|---|---|
| Size | 782.6 kB |
| Tags | CPython 3.9 macOS 10.12+ universal2 (ARM64, x86-64) macOS 10.12+ x86-64 macOS 11.0+ ARM64 |
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Release files / dima_cli-5.0.9-cp38-none-win_amd64.whl
| Download URL | dima_cli-5.0.9-cp38-none-win_amd64.whl |
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| Size | 288.4 kB |
| Tags | CPython 3.8 Windows x86-64 |
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| Download URL | dima_cli-5.0.9-cp38-cp38-manylinux_2_17_x86_64.manylinux2014_x86_64.whl |
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| Size | 447.1 kB |
| Tags | CPython 3.8 Linux glibc 2.17+ x86-64 |
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Release files / dima_cli-5.0.9-cp38-cp38-macosx_10_12_x86_64.macosx_11_0_arm64.macosx_10_12_universal2.whl
| Download URL | dima_cli-5.0.9-cp38-cp38-macosx_10_12_x86_64.macosx_11_0_arm64.macosx_10_12_universal2.whl |
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| Size | 782.3 kB |
| Tags | CPython 3.8 macOS 10.12+ universal2 (ARM64, x86-64) macOS 10.12+ x86-64 macOS 11.0+ ARM64 |
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