DNA cloning simulation for restriction based assembly and more
DNA Cauldron is a Python library to simulate restriction-based assembly operations. Provided the sequences of genetic parts and receptor vectors, DNA Cauldron will compute the assembli(es) that could result from the mix.
DNA Cauldron was written for Synthetic Biology applications - typically, to predict and validate batches of parts-based assemblies. It is simple to use, plays well with BioPython, can import and export Genbank (it conserves all features), and provides advanced methods such as connector part auto-selection, backbone selection for linear parts, methods to select constructs subsets when dealing with large combinatorial assemblies.
pip install dnacauldron
License: MIT, Copyright Edinburgh Genome Foundry
More biology software
DNA Cauldron is part of the EGF Codons synthetic biology software suite for DNA design, manufacturing and validation.