eippred 1.0.2

EIPPred: A tool for predicting,and designing MIC of the peptides

EIPPred

A computational approach to predict the MIC values of inhibitory peptides against E.coli using the amino acid sequence information.

Introduction

EIPpred is developed to predict and design the inhibitory peptides. In the standalone version, the Random Forest regressor-based model has been implemented. EIPpred is also available as a web server at https://webs.iiitd.edu.in/raghava/eippred. Please read/cite the content about the EIPpred for complete information, including the algorithm behind the approach.

Standalone

The Standalone version of transfacpred is written in python3 and following libraries are necessary for the successful run:

• scikit-learn
• Pandas
• Numpy

Minimum USAGE

To know about the available option for the stanadlone, type the following command:

eippred.py -h

To run the example, type the following command:

eippred.py -i example_input.fa

This will predict the MIC values of the submitted sequences, which will help identify the inhibitory activity of the peptides against E.coli. It will use other parameters by default. It will save the output in "outfile.csv" in CSV (comma-separated variables).

Full Usage

usage: eippred.py [-h] 
                  [-i INPUT]
                  [-o OUTPUT]
		  [-j {1,2,3}]
		  [-d {1,2}]

Please provide following arguments for successful run

optional arguments:
  -h, --help            show this help message and exit
  -i INPUT, --input INPUT
                        Input: protein or peptide sequence(s) in FASTA format or single sequence per line in single letter code
  -o OUTPUT, --output OUTPUT
                        Output: File for saving results by default outfile.csv
  -j {1,2,3}, --job {1,2,3}
                        Job Type: 1:Predict, 2: Design, by default 1
  -p POSITION, --Position POSITION
                        Position of mutation (1-indexed)
  -r RESIDUES, --Residues RESIDUES
                        Mutated residues (one or two of the 20 essential amino acids)

Input File: It allow users to provide input in the FASTA format.

Output File: The Program will save the results in the CSV format; if the user does not provide the output file name, it will be stored in "outfile.csv".

Job: User is allowed to choose between three different modules, such as, 1 for prediction, and 2 for Designing, by default its 1.

Position: User can choose any position in long sequences for mutation. This option is available for only Design module.

Residues: This option allows users to incorporate the mutation of single amino-acid and dipeptide amino-acid residue from the original peptide sequences at the specific position defined by the user.

EIPPred Package Files

It contantain following files, brief descript of these files given below

INSTALLATION : Installations instructions

LICENSE : License information

README.md : This file provide information about this package

eippred.py : Main python program

example_input.fa : Example file contain peptide sequenaces in FASTA format

example_predict_output.csv : Example output file for predict module

example_design_output.csv : Example output file for design module