famCircle
Project description
famCircle 基因家族分析,tandem可视化分析
分析流程
基因组下载以及处理 路线: 前期处理序列比对、共线性扫描、ks计算 ks展示 全基因组 ks展示 block ks展示 circle全基因组共线性展示 hmmer 隐马尔可夫模型搜索筛选 screen tandem数量分部 断开处: pfam结构域筛选# http://pfam.xfam.org/ α/β筛选# http://cb.csail.mit.edu/cb/paircoil2/ 主程序选择: inner typing 数据格式化 inner绘图 outer typing 数据格式化 outer绘图
配置文件解释
总体ks分布可视化
run ks ks = ks file vertical = False bins = 100 model = YN00/NG86 savefile = save file (*.png, *.pdf)
基因块ks分布可视化
run Ks_allocation ks = ks file area = 0,2 vertical = False model = YN00/NG86 blockfile = block file blocklength = 6 pvalue = 0.05 savefile = save file (*.png, *.pdf)
共线性可视化
run circle lens = lens file gff = gff file chrolist = Genome name ks = ks file genepairs = genepairs file peripheral = False block = 6 Ks_concern = 0,0.15 bridge = 1 radius = 0.3 savefile = save file (*.png, *.pdf)
基因家族查找
run hmmer pep = pep file# 蛋白质文件 hmmmoldpath = hmm file# 模型地址 format_conversion = Fales# 格式转换 comparison = muscle/clustal# 比对软件 e_value1 = value1# 初筛阈值 e_value2 = value2# 复筛阈值
结构域筛选
α/β筛选
结构域分布情况
run screen domainpath = domain file path lens = lens file gff = gff file chrolist = Genome name series = 25# 串联数 outpath = out file path
文件格式化
run typing domainpath = domain file# 文件路径 domainlist = Genome name# 文件名列表 position = inner/outer# 目标程序格式 savefile = out file# 保存文件
内卷型的tandem可视化
run inner lens = lens file gff = gff file chrolist = Genome name ks = ks file genefamily = famliy file Ks_concern = 0.1,0.2,1.4,1.5# ks分割参数可调 bridge = 0.05# 最远连接 radius = 0.3 peripheral = False savecsv = outer file (.csv) savefile = save file (.png, *.pdf)
放射型的tandem可视化
run outer lens = lens file gff = gff file chrolist = Genome name ks = ks file genepairs = genepairs file Ks_concern = 0,0.15# ks分割参数可调 series = 25# 串联数 clusters = None# 集团参数可调 peripheral = False bridge = 1# 跨区域连线参数可调 radius = 0.3 savecsv = outer file (.csv) savefile = save file (.png, *.pdf)
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