fetch-tool 0.8.2rc1

Utility to fetch public and private RAW read and assembly files from the ENA

Microbiome Informatics ENA fetch tool

Set of tools which allows you to fetch RAW read and assembly files from the European Nucleotide Archive (ENA).

How to set up your development environment

We recommend you to use miniconda|conda to manage the environment.

Clone the repo and install the requirements.

$ git clone [e-mail]:EBI-Metagenomics/fetch_tool.git
$ cd fetch_tool
$ # activate anv (conda activate xxx)
$ pip install -r requirements-dev.txt

Pre-commit hooks

Setup the git pre-commit hook:

pre-commit install

Why?

pre-commit will run a set of pre-configured tools before allowing you to commit files. You can find the currently configure hooks and configurations in .pre-commit-config.yaml

Tests

This repo uses pytest.

It requires the aspera cli installed in the default location (install-aspera.sh with no parameters).

To run the test suite:

pytest

Install fetch tool

Using Conda

$ conda create -q -n fetch_tool python=3.8
$ conda activate fetch_tool

Install from requirements file

$ git clone [e-mail]:EBI-Metagenomics/fetch_tool.git
$ pip install -r requirements.txt
$ pip install -U fetch_tool/

Install from Git repo

$ pip install git+ssh://[e-mail]/EBI-Metagenomics/fetch_tool.git

Install from private Git repo with access token (access token can be found in centralised password file)

$ pip install -U git+https://{access_token}@github.com/EBI-Metagenomics/fetch_tool@master

Configuration file

Setup the configuration file, the template fetchdata-config-template.json for the configuration file.

The required fields are:

• For Aspera
  • aspera_bin (the path to ascp, usually in the aspera installation under /cli/bin)
  • aspera_cert (the path to the ascp provided cert, usually in the aspera installation under /cli/etc/asperaweb_id_dsa.openssh)
• To pull private ENA data
  • ena_api_user
  • ena_api_password

Install Aspera

Install

Run the install-aspera.sh command here, it has only one optional parameter (the installation folder).

./install path/to/installation-i-want

Otherwise it will install it in $PWD/aspera-cli

Fetch read files (amplicon and WGS data)

Usage

$ fetch-read-tool -h
usage: fetch-read-tool [-h] [-p PROJECTS]
                    [-ru PROJECT_RUNS [PROJECT_RUNS ...]] [-c CONFIG_FILE]
                    -d DDIR [-f] [-w] [-r] [-l PLIST] [-v] [-o OUTPUT_FILE]
                    [-i]

Tool to fetch project sequence data from ENA

optional arguments:
    -h, --help            show this help message and exit
    -p PROJECTS, --project PROJECTS
                        Project accession(s)
    -ru PROJECT_RUNS [PROJECT_RUNS ...], --runs PROJECT_RUNS [PROJECT_RUNS ...]
                        Run accession(s) whitespace separated. That option is
                        useful if you want to download only certain project
                        runs
    -c CONFIG_FILE, --config CONFIG_FILE
                        Configuration file [json]
    -d DDIR, --dir DDIR   Base directory for downloads
    -f, --force           Force the download, even if it hits cases of submitted
                        files only. But it will not download the submitted
                        files.
    -w, --use_view        Use DB views rather than FTP
    -r, --private         Data is private
    -l PLIST, --project_list PLIST
                        Project list
    -v, --verbose         Verbose
    -o OUTPUT_FILE, --output OUTPUT_FILE
                        Output summary [json]
    -i, --interactive     interactive mode - allows you to skip failed
                        downloads.

Example

Download amplicon study:

$ fetch-read-tool -p SRP062869 -c fetchdata-config-local.json -v -d /home/<user>/temp/

Fetch assembly files

Usage

$ fetch-assembly-tool -h
usage: fetch-assembly-tool [-h] -p PROJECT
                       [-as PROJECT_ASSEMBLIES [PROJECT_ASSEMBLIES ...]]
                       [-c CONFIG_FILE] [-d DDIR] [-s {ftp,filesystem}]
                       [-v] [-pr {1.0,2.0,3.0,4.0,4.1}] [-i]

Tool to fetch assemblies from ENA

optional arguments:
  -h, --help            show this help message and exit
  -p PROJECT, --project PROJECT
                        Project accession
  -as PROJECT_ASSEMBLIES [PROJECT_ASSEMBLIES ...], --assemblies PROJECT_ASSEMBLIES [PROJECT_ASSEMBLIES ...]
                        Analysis accession(s) (e.g. ERZ773283) whitespace
                        separated. That option is useful if you want to
                        download only certain project analyses
  -c CONFIG_FILE, --config CONFIG_FILE
                        Configuration file [json]
  -d DDIR, --dir DDIR   Base directory for downloads
  -s {ftp,filesystem}, --source {ftp,filesystem}
                        Source of the RAW files.
  -v, --verbose         Verbose
  -pr {1.0,2.0,3.0,4.0,4.1}, --pipeline-version {1.0,2.0,3.0,4.0,4.1}
                        Specify pipeline version e.g. 4.1
  -i, --interactive     interactive mode - allows you to skip failed
                        downloads.

Example

Download assembly study:

$ fetch-assembly-tool -p ERP111288 -c fetchdata-config-local.json -v -d /home/<user>/temp/