flim-labs-api 1.0.0

Python API developed for controlling, acquiring and displaying time tagged fluorescence photons' data streamed from a data acquisition card based on FPGA technology in order to perform real time Fluorescence Lifetime Imaging (FLIM) and Spectroscopy applications.

flim_labs_api

What is it?

flim_labs_api is a Python API developed for controlling, acquiring and displaying time tagged fluorescence photons' data streamed from a data acquisition card based on FPGA technology in order to perform real time Fluorescence Lifetime Imaging (FLIM) and Spectroscopy applications. Overall, this API sets up a communication system between Python and a FLIM data acquisition system based on FPGA that can receive data in various modes and store it for processing.

The complete FLIM kit developed by FLIM LABS for performing Fluorescence Lifetime Spectroscopy and Imaging looks like this:

1. Fiber-coupled picosecond pulsed laser module

2. FLIM data acquisition card

3. Single-photon SPAD detector

4. FLIM studio software

How to get drivers

For getting the drivers allowing the communication with the data acquisition card email us at [e-mail].

How to get the API

You can install flim_labs_api with the requested dependencies with the following pip command:

pip install flim_labs_api

Main features

In the API five different acquisition modes are specified:

Acquisition mode	Description
Unset	This is the default value of acquisition mode
<b<Photons_tracing	Acquires the number of fluorescence photons in 100 microseconds time bins
<b<Spectroscopy	Acquires the number of fluorescence photons in 50-100 picoseconds time bins (depending on the pulsed laser's frequency) and reconstruct the fluorescence lifetime decay curve
Measure frequency	Acquires the frequency of the laser's pulses with a precision of tens/hundreds of Hz for repetition rates of tens of MHz
Acquire_raw_data	Acquires and saves the data coming from the FPGA as binary files without processing