Search for guide RNA sites in PCR tags
Project description
Search for guide RNA sites in PCR tags
Usage
- Install the package version through pip
pip install gRNAsearch from gRNAsearch.search import searchsummary, frames = search(syn="../seq.fa", tag="../tag.csv", save=True, output_dir="~", output_name="gRNA")
Parameters
search(syn, tag, save=True, output_dir='~', output_name="gRNA"):
- syn: full path to sequence file in '.fa' format
- tag: PCR tag file
- save: save the outputs in csv (default=True)
- output_dir: output directory (full path) to save the files (default='~')
- output_name: name of the output file (default="gRNA")
Outputs
- summary: the search results data frame
- frames: the intermediate data frame with WT sequences
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