genetools 0.5.0

General genetics/genomics utilities.

genetools: single-cell analysis recipes (work in progress)

Plot gallery

Other features

• Compare clustering results by computing co-clustering percentage.
• Map marker genes against reference lists to find names for your clusters.
• pandas shotrcuts:
  • Split single cell barcodes conveniently.
  • Defensive pandas merging and concatenation methods with strict correctness checks.

Full documentation: https://genetools.maximz.com.

Install

Run pip install --upgrade 'genetools[scanpy]'.

Or if you don't use scanpy: pip install --upgrade genetools.

Usage

To use genetools in a project, add import genetools. Review the documentation and the tests for examples.

Development

Setup:

git clone git://github.com/maximz/genetools
cd genetools
pip install --upgrade pip wheel
pip install -r requirements_dev.txt
pre-commit install

Common commands:

# lint
make lint

# one-time: generate test anndata, and commit so we have reproducible tests in CI
rm -r data
make regen-test-data

# run tests locally
# this is done in a debian-based docker image to ensure image style matches what Github Actions CI will produce
make build-docker-test-image # whenever requirements_dev.txt change
make test

# generate baseline figures (also happens in docker)
make regen-snapshot-figures

# regenerate test data, and baseline figures (also happens in docker)
make regen-test-data

# run tests locally without docker, therefore omitting the snapshot tests
make test-without-figures

# docs
make docs

# bump version before submitting a PR against master (all master commits are deployed)
bump2version patch # possible: major / minor / patch

# also ensure CHANGELOG.md updated

CI:

• Main: Github Actions
• Docs: https://app.netlify.com/sites/genetools

Changelog

0.5.0 (2022-01-10)

• Improve scatter plots and stacked bar plots.
• Introduce HueValueStyle for granular styling of each hue.

0.4.0 (2020-07-22)

• Centered log ratio (CLR) normalization for Cite-seq protein data.

0.3.0 (2020-06-03)

• Pandas helpers for easier normalization

0.2.0 (2020-06-03)

• Far faster implementation of stats.coclustering
• Introducing helpers.make_slurm_command
• Global submodule import (no longer need to import submodules individually)

0.1.0 (2020-03-06)

• First release on PyPI.