gff3toddbj 0.4.0

Create a DDBJ annotation file from GFF3 and FASTA files

GFF3-to-DDBJ

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[TOC]

Table of Contents

• What is this?
• How accurate is the conversion?
• Initial setup
  • Install with bioconda
  • Install with pip
  • Install from the source
• Create DDBJ annotation from GFF3 and FASTA
  • Run gff3-to-ddbj
• Under the Hood
• Customize the behavior
  • Metadata file
  • [Advanced] Rename features and qualifiers
    • Rename types/feature keys
    • Rename attributes/qualifier keys
    • Translate GFF3 types to features with qualifiers
    • Translate (type, attribute) items to features
    • Run with custom configuration
  • [Advanced] Filter features and qualifiers
• Troubleshooting
  • Validate GFF3
  • Split FASTA from GFF3 (if needed)
  • Normalize entry names (if needed)
• Known Problems
• Credit

Table of contents generated with markdown-toc

What is this?

GFF3-to-DDBJ creates the annotation file for submission to DDBJ by taking GFF3 and FASTA files as input. It also works with FASTA alone.

Analogous programs are table2asn and GAG for submissions to NCBI, and EMBLmyGFF3 for submissions to EMBL.

Please take a look at our test directory for examples. Files ending with .ann are the DDBJ annotation files produced by thie program.

How accurate is the conversion?

While there are many rules a DDBJ annotation file needs to comply with, it's difficult to tell what the correct GFF3→DDBJ conversion is. There is no example of full-fledged GFF3 → DDBJ conversion available, either. So, we define GFF3-GenBank correspondence in RefSeq as the "correct" examples. To evaluate GFF3-to-DDBJ, we use RefSeq data and compare gff3-to-ddbj output with the other DDBJ annotation from genbank-to-ddbj using the GenBank format. Please take a look at our evaluation dcoument for the detail as well as the current status. ([TODO] Add the page...)

Here genbank-to-ddbj is an executable included in this package. It shares codebase with gff3-to-ddbj, but we believe it does not bring any complexity to our evaluation due to its much simpler internals.

Also note that we also use DDBJ's Parser for checking the annotation files.

Initial setup

Install with bioconda

# Create a conda environment named "ddbj", and install relevant packages from bioconda channel
conda create -n ddbj -c bioconda -c conda-forge gff3toddbj

# Activate the environment "ddbj"
conda activate ddbj

Install with pip

# Create a conda environment named "ddbj" and install pip
conda create -n ddbj pip

# Activate the environment "ddbj"
conda activate ddbj

# Need bgzip executable in samtools
conda install -c bioconda samtools

# Install from pip
pip install gff3toddbj

Install from the source

# Download
wget https://github.com/yamaton/gff3_to_ddbj/archive/refs/heads/main.zip

# Extract, rename, and change directory
unzip main.zip && mv gff3toddbj-main gff3toddbj && cd gff3toddbj

# Create a conda environment named "ddbj"
conda create -n ddbj

# Activate the environment "ddbj"
conda activate ddbj

# Install dependencies to "ddbj"
conda install -c bioconda -c conda-forge biopython bcbio-gff toml pysam samtools pip build

# Install gff3-to-ddbj and extra tools
python -m build && pip install -e ./

Create DDBJ annotation from GFF3 and FASTA

Run gff3-to-ddbj

Let's run the main program to get some ideas.

gff3-to-ddbj \
  --gff3 myfile.gff3 \                # bare-minimum output if omitted
  --fasta myfile.fa \                 # <<REQUIRED>>
  --metadata mymetadata.toml \        # example metadata used if omitted
  --locus_tag_prefix MYOWNPREFIX_ \   # default is "LOCUSTAGPREFIX_"
  --transl_table 1 \                  # default is 1
  --output myawesome_output.ann       # standard output if omitted

Here is the options:

• --gff3 <FILE> takes GFF3 file
• --fasta <FILE> takes FASTA file
• --metadata <FILE> takes the metadata file in TOML
• --locus_tag_prefix <STRING> takes the prefix of locus tag obtained from BioSample. You can skip this for now.
• --transl_table <INT>: Choose appropriate one from The Genetic Codes. The default value is 1 ("standard").
• --output <FILE> sets the path the annotation output.

Under the Hood

Here is the list of operations gff3-to-ddbj will do:

• Re-compress FASTA with bgzip if the FASTA input is compressed with gzip

  • A bgzip file is created if absent like myfile_bgzip.fa.gz.
  • For indexing and saving memory
  • The bgzip file should be compatible with gzip

• Rename features and qualifiers following the renaming scheme.

• Search for assembly_gaps in FASTA, and add the feature.

• Add /transl_table to each CDS.

• Insert source information from the metadata fie.

• Insert TOPOLOGY feature if GFF3 has Is_circular=true in an entry.

  • Also handle origin-spaning features.

• Join locations of features having the same parent with join notation.

  • CDS, exon, mat_peptide, V_segment, C_region, D-loop, and misc_feature may be joined.
  • exons are NOT joined if having gene as the direct parent.

• Set the location of joined exons as its parent RNA's location, and discard the exons.

• Add partialness markup (< and >) to CDS locations if start/stop codon is absent.

  • See Offset of the frame at translation initiation by codon_start

• Let CDS have a single /product value: Set it to "hypothetical protein" if absent. Move the rest of exising values to /note.

  • This is to conform the instruction on /product.

    • Even if there are multiple general names for the same product, do not enter multiple names in 'product'. Do not use needless symbolic letters as delimiter for multiple names. If you would like to describe more than two names, please enter one of the most representative name in /product qualifier, and other(s) in /note qualifier.

    • If the name and function are not known, we recommend to describe as "hypothetical protein".

• If a gene feature has /gene and/or /gene_synonym, copy these qualifiers to its children.

• Make /gene have a single value, and put the rest to /gene_synonym.

  • Reference: Definition of Qualifier key: /gene.

• Remove duplicates in qualifier values.

• Sort lines in annotation

• Filter features and qualifiers following the matrix.

  • gene feature will be discarded in this process.

Customize the behavior

Metadata file

To enter information missing in GFF3 or FASTA, such as submitter names and certain qualifier values, you need to feed a metadata file in TOML, say mymetadata.toml. Take a look at an example matching the example annotation in the DDBJ page.

The file accommodates following and they are all optional. That is, GFF3-to-DDBJ works even with an empty file.

• Basic features in the COMMON entry

  • ... such as SUBMITTER, REFERENCE, and COMMENT.

• "meta-description" in the COMMON entry

  • Here is an example with this notation:

    [COMMON.assembly_gap]
    estimated_length = "unknown"
    gap_type = "within scaffold"
    linkage_evidence = "paired-ends"
    

  • DDBJ annotation supports "meta" values with features under COMMON such that the items are inserted to each occurrence in the resulting flat file produced by DDBJ. Here is an example to insert assembly_gap feature under COMMON entry.

• Feature-qualifier items inserted to each occurrence

  • Here is an example: Difference from the previous case is only at [assembly_gap] as opposed to[COMMON.assembly_gap].

    [assembly_gap]
    estimated_length = "unknown"   # Set it "<COMPUTE>" to count the number of N's
    gap_type = "within scaffold"
    linkage_evidence = "paired-ends"
    

  • While this should work effectively the same as the "meta-description" item above, use this notation if you insert values repeatedly in the annotation file produced by GFF3-to-DDBJ.

  • Currently supporting [source] and [assembly_gap] only.

For more examples, see WGS in COMMON and WGS provided by DDBJ as annotation examples, and corresponding metadata files metadata_WGS_COMMON.toml and metadata_WGS.toml in this repository.

[Advanced] Rename Features and Qualifiers

GFF3 and DDBJ annotation have rough correspondence like:

1. GFF3 column 3 "type" → DDBJ annotation column 2 as "Feature"
2. GFF3 column 9 "attribute" → DDBJ annotation column 4 and 5 as "Qualifier key", and "Qualifier value"

but nomenclatures in GFF3 often do not conform the annotations set by INSDC. Furthermore, DDBJ lists up the feature-qualifier pairs they accepts, a subset of the INSDC definitions.

To meet convensions with the requirement, GFF3-to-DDBJ comes with a default configuration in TOML to rename (or even translate) feature keys and qualifier keys/values. Note that the Sequence Ontology is helpful in translating a type into a INSDC feature and qualifier(s).

Here is how to customize the renaming configuration.

Rename types/feature keys

The default setting renames five_prime_UTR "type" in GFF3 into 5'UTR "feature key" in the annotation. This transformation is expressed in TOML as follows:

[five_prime_UTR]
feature_key = "5'UTR"

Rename attributes/qualifier keys

This is about renaming attributes under arbitrary types. By default, ID=foobar "attribute" in a GFF3 becomes /note="ID:foobar" qualifier in the annotation. (Here I follow the convention putting slash (like /note) to denote qualifier. But DDBJ annotation does NOT include slash hence no slash is used in any of TOML files.)

Here is the TOML defining the transformation. __ANY__ is the special name representing arbitrary types. ID is the original attribute key. note is the name of corresponding qualifier key. ID: is attached as the prefix of the qualifier value.

[__ANY__.ID]
qualifier_key = "note"
qualifier_value_prefix = "ID:"  # optional

One can also set a qualifier key and a value together. For example, /pseudo qualifier is discouraged by DDBJ regardless of features. We may enforce the replacement by,

# /pseudo is always replaced by /pseudogene="unknown"
[__ANY__.pseudo]
qualifier_key = "pseudogene"
qualifier_value = "unknown"

Translate GFF3 types to features with qualifiers

Sometimes we want to replace a certain types with features WITH qualifiers. For example, snRNA is an invalid feature in INSDC/DDBJ hence we replace it with ncRNA feature with /ncRNA_class="snRNA" qualifier. Such transformation is written in TOML as following.

[snRNA]
feature_key = "ncRNA"
qualifier_key = "ncRNA_class"
qualifier_value = "snRNA"

Translate (type, attribute) items to features

Here is a story in setting the default renaming scheme: Some annotation programs produce a GFF3 line containing RNA as the type and biotype=misc_RNA as one of the attributes. But it should be treated as misc_RNA feature in DDBJ annoation. In such case, we join (feature key, qualifier key, qualifier value) with dot as delimiter, and write as follows.

[RNA.biotype.misc_RNA]
feature_key = "misc_RNA"

Run with custom configuration

To feed a custom translation table, use the CLI option:

• --config_rename <FILE>

And here is an example call:

gff3-to-ddbj \
  --gff3 myfile.gff3 \
  --fasta myfile.fa \
  --metadata mymetadata.toml \
  --locus_tag_prefix MYOWNPREFIX_ \
  --transl_table 1 \
  --config_rename my_translate_features_qualifiers.toml \  # Set your customized file here
  --output myawesome_output.ann

[Advanced] Filter features and qualifiers

DDBJ specifies recommended Feature/Qualifier usage matrix. To conform this rule, features and qualifiers appearing in the annotation output are filtered by the filtering file in TOML by default. The file is in TOML format with the structure like this:

CDS = [
"EC_number",
"inference",
"locus_tag",
"note",
"product",
]

exon = [
"gene",
"locus_tag",
"note",
]

The left-hand side of the equal sign = represents an allowed feature key, and the right-hand side is a list of allowed qualifier keys. In this example, only CDS and exon features will show up in the annotation, and qualifiers are limited to the listed items. To customize this filtering function, edit the TOML file first and pass the file with the CLI option:

• --config_filter <FILE>

Troubleshooting

Validate GFF3

It might be a good practice to validate your GFF3 files. GFF3 online validator is useful though the file size is limited to 50MB.

Split FASTA from GFF3 (if needed)

GFF3_to_DDBJ does not work when GFF3 contains FASTA information inside with ##FASTA directive. Attached tool split-fasta reads a GFF3 file and saves GFF3 (without FASTA info) and FASTA.

split-fasta path/to/myfile.gff3 --suffix "_splitted"

This creates two files, myfile_splitted.gff3 and myfile_splitted.fa.

Normalize entry names (if needed)

Letters like =|>" [] are not allowed in the 1st column (= "Entry") of the DDBJ annotation. The attached program normalize-entry-names renames such entries. This program converts an ID like ERS324955|SC|contig000013 into ERS324955:SC:contig000013 for example.

normalize-entry-names myannotation_output.txt

This command create as files myannotation_output_renamed.txt if the invalid letters are found. Otherwise, you'll see no output.

Known Issues

• Need to handle location correction and feature join() in presence of /trans_splicing
• Need to handle location correction in presence of /transl_except at start/stop codon
• Needs /translation when /exception exists.
• GFF3 handling when the flatfile is supposed to have "between-position" location like 123^124
• Currently the development focuses on accuracy; the software runs slow using a single process.

Credit

GFF3-to-DDBJ's design is deeply indebted to EMBLmyGFF3, a versatile coversion for EMBL annotation format.