DNA overhang design for Golden Gate etc.
Golden Hinges (full documentation here) is a Python library to find sets of overhangs (also called junctions, or protrusions) for multipart DNA assembly such as Golden Gate assembly.
Given a set of constraints (GC content bounds, differences between overhangs, mandatory and forbidden overhangs) Golden Hinges enables to find:
Maximal sets of valid and inter-compatible overhangs.
Sequence decompositions (i.e. position of cuts) which produce valid and inter-compatible overhangs, for type-2S DNA assembly.
Sequence mutations (subject to constraints) which enable the sequence decomposition, in extreme cases where the original sequence does not allow for such decomposition.
pip install goldenhinges
Github Page (with examples)
License: MIT, Copyright Edinburgh Genome Foundry
More biology software
Golden Hinges is part of the EGF Codons synthetic biology software suite for DNA design, manufacturing and validation.
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