harmonypy
harmonypy is a Python package for the Harmony algorithm for integrating multiple high-dimensional datasets. It uses a C++ backend (Armadillo) for fast linear algebra, matching the R harmony2 package step-by-step.
This animation shows Harmony aligning three single-cell RNA-seq datasets from different donors. → How to make this animation. Before Harmony, you can clearly distinguish cells from each of the three donors. After Harmony, the cells from different donors are mixed while preserving the overall shape of the data.
Installation
Install from PyPI (pre-built wheels for Linux and macOS):
pip install harmonypy
Building from source
Building from source requires a C++ compiler, CMake, and a BLAS library:
macOS (uses Apple Accelerate, no extra dependencies):
pip install .
Linux (requires OpenBLAS):
# Debian/Ubuntu
sudo apt install libopenblas-dev cmake
# RHEL/Fedora
sudo dnf install openblas-devel cmake
pip install .
Quick Start
import harmonypy as hm
import pandas as pd
# Load the principal components and metadata
pcs = pd.read_csv("data/pbmc_3500_pcs.tsv.gz", sep="\t")
meta = pd.read_csv("data/pbmc_3500_meta.tsv.gz", sep="\t")
# Run Harmony to correct for batch effects (donor)
harmony_out = hm.run_harmony(pcs, meta, "donor")
# Save corrected PCs (same shape as input)
result = pd.DataFrame(harmony_out.Z_corr, columns=pcs.columns)
result.to_csv("pbmc_3500_pcs_harmony.tsv", sep="\t", index=False)
Usage with Scanpy
import scanpy as sc
import harmonypy as hm
# Load and preprocess your data
adata = sc.read_h5ad("my_data.h5ad")
sc.pp.pca(adata)
# Get PCs from the AnnData object
pcs = adata.obsm['X_pca']
print(pcs.shape) # (n_cells, n_pcs)
# Run Harmony on the PCA embedding
harmony_out = hm.run_harmony(pcs, adata.obs, "batch")
# Store corrected PCs back in the AnnData object
adata.obsm['X_pca_harmony'] = harmony_out.Z_corr
# Use harmonized PCs for downstream analysis
sc.pp.neighbors(adata, use_rep='X_pca_harmony')
sc.tl.umap(adata)
sc.tl.leiden(adata)
Parameters
run_harmony accepts the same parameters as the R package:
| Parameter | Default | Description |
|---|---|---|
theta |
2 | Diversity penalty per batch variable |
sigma |
0.1 | Kernel bandwidth for soft clustering |
nclust |
min(N/30, 100) | Number of clusters |
max_iter_harmony |
10 | Maximum Harmony iterations |
max_iter_kmeans |
4 | K-means iterations per Harmony round |
epsilon_harmony |
1e-2 | Convergence threshold |
ncores |
0 | BLAS threads (0 = all cores) |
lamb |
None | Ridge penalty (None = auto-estimate) |
The ncores parameter controls BLAS threading (Accelerate on macOS, OpenBLAS on Linux). Default is 0 (use all available cores). Set ncores=1 for single-threaded execution.
Performance
The script in tests/test_harmony.py on an Apple M1 (2022) chip reports:
Dataset Time RSS delta
---------------------- -------- ------------
Small (3.5k cells) 0.23s 45.2 MB
Medium (69k cells) 4.76s 262.3 MB
Large (858k cells) 29.29s 1969.5 MB
Citation
If you use Harmony in your work, please cite the original paper:
Korsunsky, I., Millard, N., Fan, J. et al. Fast, sensitive and accurate integration of single-cell data with Harmony. Nat Methods 16, 1289–1296 (2019). https://doi.org/10.1038/s41592-019-0619-0
The Supplementary Information PDF provides detailed mathematical descriptions and implementation notes.
To learn more about Harmony 2, please see the preprint here:
Patikas, Nikolaos, Hongcheng Yao, Roopa Madhu, Soumya Raychaudhuri, Martin Hemberg, and Ilya Korsunsky. 2026. Integration of Large, Complex Single-Cell Datasets with Harmony2. bioRxiv. https://doi.org/10.64898/2026.03.16.711825
1 maintainer
Release files for harmonypy 2.0.2
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Source distribution (sdist)
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Built distributions (wheels)
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| Tags | CPython 3.9 Linux glibc 2.28+ ARM64 |
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| Size | 155.8 kB |
| Tags | CPython 3.9 macOS 11.0+ ARM64 |
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