ispcr 0.6.0

Simple in silico PCR

ispcr

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Documentation: https://pommevilla.github.io/ispcr

Source Code: https://github.com/pommevilla/ispcr

PyPI: https://pypi.org/project/ispcr/

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A simple, light-weight package written in base Python to perform in silico PCR to determine primer performance.

Currently in development

Installation

pip install ispcr

Demonstration

The main function to use in this package is find_pcr_product, which takes as input two file paths:

• primer_file - the path to fasta file containing your primers
  • This is currently limited to a fasta file containing two sequences, with the forward primer coming first and the reverse primer coming second
• sequence_file the path to the fasta file containing the sequences to test your primers against

find_pcr_product will then iterate through the sequences in sequence file and find all products amplified by the forward and reverse primer.

find_pcr_product also takes a minimum_product_length argument: