Jupyter Notebooks extension for showing JBrowse views
Project description
JBrowse Jupyter
JBrowse Jupyter is a python package that provides a python interface to JBrowse views.
The package provides a JBrowseConfig API to enable the creation of JBrowse state configuration objects. It also provides utility functions to create and embed Dash JBrowse components in jupyter notebooks and python applications.
You can open this browser.ipynb in colab here Note: to install the package in colab you can use this command. In a new cell above the import statement
!pip install git+https://github.com/GMOD/jbrowse-jupyter.git@main
Dash JBrowse
Dash JBrowse is a collection of dash components for JBrowse's embeddable components.
We utilize the Dash JBrowse package along with jupyter-dash library to embed JBrowse React Linear Genome view or the JBrowse React Circular Genome view in any jupyter notebook.
You can find more information about our Dash JBrowse library here.
Demos
- Browser notebook demo - https://colab.research.google.com/github/GMOD/jbrowse-jupyter/blob/main/browser.ipynb
- SK-BR-3 demo - https://colab.research.google.com/github/GMOD/jbrowse-jupyter/blob/main/skbr3.ipynb
Documentation
Additional details and tutorials can be found in our Sphinx documentation page. https://gmod.github.io/jbrowse-jupyter/docs/html/index.html
Installation
jbrowse-jupyter
is freely available for download on the Python Package Index
https://pypi.org/project/jbrowse-jupyter/
Pre-requisites
- conda - for virtual environments
- pip - python package manager
- Python - 3.6 or greater
- Jupyter - jupyter lab or jupyter notebook to run .ipynb files
PyPI
$ pip install jbrowse-jupyter
Install with conda
Clone this repository and install conda. Once you have conda installed, follow the steps found below to create a conda envirnment and install the dependecies.
$ cd jbrowse-jupyter
$ conda create -n myenv
$ conda activate myenv
$ pip install -r requirements.txt
Quickstart
Install the JBrowse Jupyter package using pip
$ pip install jbrowse-jupyter
Launching a Linear Genome View in Jupyter Notebook
Launching a Circular Genome View in Jupyter Notebook JBrowse Linear Genome view in python Dash application
from dash import html, Dash
from jbrowse_jupyter import create, create_component
app = Dash(__name__)
jbrowse_conf = create("LGV", genome="hg38")
config = jbrowse_conf.get_config()
component = create_component(config)
app.layout = html.Div(
[component],
id='test'
)
if __name__ == "__main__":
app.run_server(port=8081, debug=True)
using jupyter-server-proxy
or using the jupyter-server-proxy extension and the jupyter-dash extension
Using Pypi
$ pip install jupyter-server-proxy
$ pip install jupyter-dash
Using conda
$ conda install jupyter-server-proxy -c conda-forge
$ conda install -c conda-forge -c plotly jupyter-dash
$ jupyter lab build
from jupyter_dash import JupyterDash
from dash import html
from jbrowse_jupyter import create, create_component
import jupyter_server_proxy
JupyterDash.infer_jupyter_proxy_config()
#JupyterDash()._server_proxy # true if the proxy works as expected
#JupyterDash().config # gives the proxy config
app = JupyterDash(__name__)
jbrowse_conf = create("LGV", genome="hg38")
config = jbrowse_conf.get_config()
component = create_component(config)
app.layout = html.Div(
[component],
id='test'
)
if __name__ == "__main__":
# the external mode should display a url proxied by jupyter-server-proxy,
# very handy on jupyterlab behind jupyterhub as in departmental jupyterlab instances.
app.run_server(mode='external', debug=True, port=8999)
You can customize the Linear Genome View by modifying the jbrowse_conf
. The jbrowse_conf
is an instance of our JBrowseConfig
, and can be modified to set an assembly, add tracks, set custom color palettes and more.
Find more information about the JBrowseConfig API here
Other Examples
You can find examples in the root of this repo,
browser.py
- uses the Dash library to create a python application with the Dash JBrowse LinearGenomeView componentbrowser.ipynb
- jupyter notebook using the JupyterDash library to embed a Dash JBrowse LinearGenomeView component in a cellcgv_examples.py
- uses the Dash library to create a python application with the Dash JBrowse CircularGenomeView componentcgv_examples.ipynb
- jupyter notebook using the JupyterDash library to embed a Dash JBrowse CircularGenomeView component in a cell
To run the Python Dash application
$ python browser.py
To run the jupyter notebook
Make sure you have jupyter notebook or jupyterlab.
Within the myenv conda environment
$ pip install jupyterlab
More information on using a specific environment in a jupyter notebook
Usage
The jbrowse-jupyter
package provides several utility functions to create and launch Dash JBrowse components in python applications and jupyter notebooks.
Configuring Components
create
(view_type, **kwargs)- creates a JBrowseConfig configuration object given a view_type- view_type: Choose from a LGV or CGV e.g
create
('LGV') orcreate
('CGV') Additional params:- genome: choose from one of our default genomes {hg19 or hg38} e.g
create
('LGV', genome="hg38") OR - conf: use a conf object, you can manually edit and pass json object.
e.g
create
('LGV', conf={"my-conf": "object"}) Note: you can manually create a conf following the https://jbrowse.org/jb2/docs/config_guide/ - if no genome or conf is passed, you will create an empty JBrowseConfig for that view type. defaults if you pass no params,an empty JBrowse config for a LGV (LinearGenomeView) will be created
- genome: choose from one of our default genomes {hg19 or hg38} e.g
- view_type: Choose from a LGV or CGV e.g
create_component
(conf, **kwargs) - creates and returns a Dash JBrowse component -> 'CGV' or 'LGV'. This component can be used as any Dash component in Dash applications.- conf: JBrowseConfig obj
- id: id for Dash components (opyional)
- dash_comp: 'CGV' or 'LGV', defaults to 'LGV' when none is passed
e.g
create_component
(cgv_with_conf.get_config(), dash_comp="CGV")
launch
(conf, **kwargs) - launches a LinearGenomeView Dash component in a Jupyter cell- id: id for Dash components
- dash_comp: 'CGV' or 'LGV', defaults to 'LGV' when none is passed
Warning: Only use
launch
in jupyter notebooks
JBrowseConfig
Quick overview of the JBrowseConfig API
The JBrowseConfig API allows us to set an assembly, add tracks, set default sessions, set custom color themes, and more.
JBrowseConfig().
-
set_assembly
(assembly_data, aliases, refname_aliases)- Sets the assembly subconfiguration
- for the refname_alias subconfiguration check out the JBrowse refname aliasing docs
-
add_df_track
(track_data, name, **kwargs)- requires DataFrame to have columns ['refName', 'start', 'end', 'name']
- refName and name columns must be strings, start and end must be int
- if the score column is present, it will create a Quantitative track else it will create a Feature track.
- not available for CGV
- params:
- df – pandas DataFrame with the track data.
- name (str) – (optional) name for the track
- overwrite (str) – (optional) flag wether or not to overwrite existing track.
- track_id (str) - (optional) trackId for the track
-
add_track
(data, **kwargs)- adds a track configuration given a file with track data
- currently supporting cram, bam, vcf gzipped, gff/gff3 gzipped, bigbed, bigwig file types.
- assumes an index exists within the same directory of the track data if no index url path is provided.
- currently supporting Wiggle, Variant, Feature and Alignments tracks
- params:
- data (str) – track file or url (currently only supporting url)
- name (str) – (optional) name for the track
- index (str) – (optional) index file for the track
- track_type (str) – (optional) track type. If none is passed, the api will infer one based on the file type
- overwrite (boolean) – (optional) overwrites existing track if it exists in list of tracks (default False)
-
set_location
(location)- initial location for when the browser first loads, syntax 'refName:start-end'
- e.g 'chrom1:500-1000'
- not available for CGV
-
set_default_session
(tracks_ids, display_assembly=True)- sets the default session given a list of track ids
- params:
- tracks_ids - list[str] list of track ids to display
- display_assembly (boolean) – display the assembly reference sequence track. default=True
-
set_theme
(primary, secondary=None, tertiary=None, quaternary=None)- sets the theme in the configuration given up to 4 hexadecimal colors
-
add_text_search_adapter
(ix_path, ixx_path, meta_path, adapter_id=None)- adds a trix text search adapter
- not available for CGV
-
get_config
() - returns the configuration object
Resources
- JBrowse - the next generation genome browser
- JBrowse React Linear Genome View - interactive genome browser
- JBrowse React Linear Genome View Docs - storybook docs of React LGV
- Dash Applications how to get started to custumize Dash applications.
- Dash HTML components Dash html components to build the Dash aplication layout.
- DashJupyter library to enable embedding Dash components in jupyter notebooks.
- DashJbrowse suite of Dash components for JBrowse views. (JBrowse Linear Genome View)
Academic Use
This package was written with funding from the NHGRI as part of the JBrowse project. If you use it in an academic project that you publish, please cite the most recent JBrowse paper, which will be linked from jbrowse.org.
Contact us
We really love talking to our users. Please reach out with any thoughts you have on what we are building!
- Report a bug or request a feature at https://github.com/GMOD/jbrowse-jupyter/issues
- Join our developers chat at https://gitter.im/GMOD/jbrowse2
- Send an email to our mailing list at
gmod-ajax@lists.sourceforge.net
FAQ
-
What file types are supported?
- We currently support:
- bam
- big wig
- big bed
- cram
- fasta indexed
- fasta gzipped
- gff3 tabix
- two bit
- vcf
- vcf gzipped
- We currently support:
-
What track types are supported?
- We currently support:
- AlignmentsTrack
- QuantitativeTrack
- VariantTrack
- ReferenceSequenceTrack
- Feature Tracks
For the circular genome view (CGV), we only support variant tracks. Check out track types docs for more information.
- We currently support:
-
What views do you currently support?
- We currently support JBrowse's Linear Genome View and Circular Genome View. We hope to support more in the future.
-
How do I configure text searching?
- In order to configure text searching in your Linear Genome View, you must first create a text index. Follow the steps found here. Then you must create and add a text search adapter to your config.
-
How do I configure tracks to show up on first render?
- You can set a specific track/tracks to show up when the component first renders, and you can do this via the default session. You can set the default session via the JBrowseConfig API.
set_default_session
- You can set a specific track/tracks to show up when the component first renders, and you can do this via the default session. You can set the default session via the JBrowseConfig API.
-
How do I set a custom color theme palette to fit with my application?
- You can customize the color palette of the component through the use of
set_theme
function from the JBrowseConfig API. Below is an image of an LGV with a custom color palette.
- You can customize the color palette of the component through the use of
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