kitsune 1.3.2

A toolkit for evaluation of the lenght of k-mer in a given genome dataset for alignment-free phylogenimic analysis

KITSUNE: K-mer-length Iterative Selection for UNbiased Ecophylogenomics

KITSUNE is a toolkit for evaluation of the length of k-mer in a given genome dataset for alignment-free phylogenimic analysis.

K-mer based approach is simple and fast yet has been widely used in many applications including biological sequence comparison. However, selection of an appropriate k-mer length to obtain a good information content for comparison is normally overlooked. The optimum k-mer length is a prerequsite to obtain biological meaningful genomic distance for assesment of phylogenetic relationships. Therefore, we have developed KITSUNE to aid k-mer length selection process in a systematic way, based on a three-steps aproach described in Viral Phylogenomics Using an Alignment-Free Method: A Three-Step Approach to Determine Optimal Length of k-mer.

KITSUNE will calculte the three matrices across considered k-mer range:

1. Cumulative Relative Entropy (CRE)
2. Averrage number of Common Feature (ACF)
3. Obserbed Common Feature (OCF)

Moreover, KITSUNE also provides various genomic distance calculations from the k-mer frequency vectors that can be used for species identification or phylogenomic tree construction.

Note: If you use KITSUNE in your research, please cite: KITSUNE: A Tool for Identifying Empirically Optimal K-mer Length for Alignment-free Phylogenomic Analysis

Installation

Kitsune is developed under python version 3 environment. We recommend users use python >= v3.5.

Requirement packages: scipy >= 0.18.1, numpy >= 1.1.0, tqdm >= 4.32

Kitsune also requires Jellyfish for k-mer counting as external software dependency. Thus, you need to install it before running the tool: https://github.com/gmarcais/Jellyfish

Install with pip

pip install kitsune

Install from source

# Clone the GitHub repository
git clone https://github.com/natapol/kitsune

# Move to the kitsune folder
cd kitsune/

# Install
python setup.py install

Usage

Overview of kitsune

command for listing help

$ kitsune --help

usage: kitsune <command> [<args>]

Available commands:
  acf      Compute average number of common feature between signatures
  cre      Compute cumulative relative entropy
  dmatrix  Compute distance matrix
  kopt     Compute recommended choice (optimal) of kmer within a given kmer interval for a set of genomes using the cre, acf and ofc
  ofc      Compute observed feature frequencies

Use --help in conjunction with one of the commands above for a list of available options (e.g. kitsune acf --help)

Calculate CRE, ACF, and OFC value for specific kmer

Kitsune provides three commands to calculate an appropiate k-mer using CRE, ACF, and OCF:

Calculate CRE

$ kitsune cre -h

usage: kitsune (cre) [-h] --filename FILENAME [--fast] [--canonical] -ke KEND
                     [-kf KFROM] [-t THREAD] [-o OUTPUT]

Calculate k-mer from cumulative relative entropy of all genomes

optional arguments:
  -h, --help            show this help message and exit
  --filename FILENAME   A genome file in fasta format (default: None)
  --fast                Jellyfish one-pass calculation (faster) (default:
                        False)
  --canonical           Jellyfish count only canonical mer (default: False)
  -ke KEND, --kend KEND
                        Last k-mer (default: None)
  -kf KFROM, --kfrom KFROM
                        Calculate from k-mer (default: 4)
  -t THREAD, --thread THREAD
  -o OUTPUT, --output OUTPUT
                        Output filename (default: None)

Calculate ACF

$ kitsune acf -h

usage: kitsune (acf) [-h] --filenames FILENAMES [FILENAMES ...] [--fast]
                     [--canonical] -k KMERS [KMERS ...] [-t THREAD]
                     [-o OUTPUT]

Calculate an average number of common feature pairwise between one genome
against others

optional arguments:
  -h, --help            show this help message and exit
  --filenames FILENAMES [FILENAMES ...]
                        Genome files in fasta format (default: None)
  --fast                Jellyfish one-pass calculation (faster) (default:
                        False)
  --canonical           Jellyfish count only canonical mer (default: False)
  -k KMERS [KMERS ...], --kmers KMERS [KMERS ...]
                        Have to state before (default: None)
  -t THREAD, --thread THREAD
  -o OUTPUT, --output OUTPUT
                        Output filename (default: None)

Calculate OFC

$ kitsune ofc -h

usage: kitsune (ofc) [-h] --filenames FILENAMES [FILENAMES ...] [--fast]
                     [--canonical] -k KMERS [KMERS ...] [-t THREAD]
                     [-o OUTPUT]

Calculate an observe feature frequency

optional arguments:
  -h, --help            show this help message and exit
  --filenames FILENAMES [FILENAMES ...]
                        Genome files in fasta format (default: None)
  --fast                Jellyfish one-pass calculation (faster) (default:
                        False)
  --canonical           Jellyfish count only canonical mer (default: False)
  -k KMERS [KMERS ...], --kmers KMERS [KMERS ...]
  -t THREAD, --thread THREAD
  -o OUTPUT, --output OUTPUT
                        Output filename (default: None)

General Example

kitsune cre --filenames genome1.fna -kf 5 -ke 10
kitsune acf --filenames genome1.fna genome2.fna -k 5
kitsune ofc --filenames genome_fasta/* -k 5

Calculate genomic distance at specific k-mer from kmer frequency vectors of two of genomes

Kitsune provides a commands to calculate genomic distance using different distance estimation method. Users can assess the impact of a selected k-mer length on the genomic distnace of choice below.

distance option	name
braycurtis	Bray-Curtis distance
canberra	Canberra distance
chebyshev	Chebyshev distance
cityblock	City Block (Manhattan) distance
correlation	Correlation distance
cosine	Cosine distance
euclidean	Euclidean distance
jensenshannon	Jensen-Shannon distance
sqeuclidean	Squared Euclidean distance
dice	Dice dissimilarity
hamming	Hamming distance
jaccard	Jaccard-Needham dissimilarity
kulsinski	Kulsinski dissimilarity
rogerstanimoto	Rogers-Tanimoto dissimilarity
russellrao	Russell-Rao dissimilarity
sokalmichener	Sokal-Michener dissimilarity
sokalsneath	Sokal-Sneath dissimilarity
yule	Yule dissimilarity
mash	MASH distance
jsmash	MASH Jensen-Shannon distance
jaccarddistp	Jaccard-Needham dissimilarity Probability
euclidean_of_frequency	Euclidean distance of Frequency

Kitsune provides a choice of distance transformation proposed by Fan et.al.

Calculate a distance matrix

$ kitsune dmatrix -h

usage: kitsune (dmatrix) [-h] [--filenames [FILENAMES [FILENAMES ...]]]
                         [--fast] [--canonical] -k KMER [-i INPUT] [-o OUTPUT]
                         [-t THREAD] [--transformed]
                         [-d {braycurtis,canberra,jsmash,chebyshev,cityblock,correlation,cosine,dice,euclidean,hamming,jaccard,kulsinsk,matching,rogerstanimoto,russellrao,sokalmichener,sokalsneath,sqeuclidean,yule,mash,jaccarddistp}]
                         [-f FORMAT]

Calculate a distance matrix

optional arguments:
  -h, --help            show this help message and exit
  --filenames [FILENAMES [FILENAMES ...]]
                        Genome files in fasta format (default: None)
  --fast                Jellyfish one-pass calculation (faster) (default:
                        False)
  --canonical           Jellyfish count only canonical mer (default: False)
  -k KMER, --kmer KMER
  -i INPUT, --input INPUT
                        List of genome files in txt (default: None)
  -o OUTPUT, --output OUTPUT
                        Output filename (default: None)
  -t THREAD, --thread THREAD
  --transformed
  -d {braycurtis,canberra,jsmash,chebyshev,cityblock,correlation,cosine,dice,euclidean,hamming,jaccard,kulsinsk,matching,rogerstanimoto,russellrao,sokalmichener,sokalsneath,sqeuclidean,yule,mash,jaccarddistp}, --distance {braycurtis,canberra,jsmash,chebyshev,cityblock,correlation,cosine,dice,euclidean,hamming,jaccard,kulsinsk,matching,rogerstanimoto,russellrao,sokalmichener,sokalsneath,sqeuclidean,yule,mash,jaccarddistp}
  -f FORMAT, --format FORMAT

Example of choosing distance option:

kitsune dmatrix --filenames genome1.fna genome2.fna -k 11 -d jaccard --canonical --fast -o output.txt
kitsune dmatrix --filenames genome1.fna genome2.fna -k 11 -d hensenshannon --canonical --fast -o output.txt

Find optimum k-mer from a given set of genomes

Kitsune provides a wrap-up comand to find optimum k-mer length for a given set of genome within a given kmer interval.

$ kitsune kopt -h

usage: kitsune (ofc) [-h] --filenames FILENAMES [--fast] [--canonical] -kl
                     KLARGE [-o OUTPUT] [--closely_related] [-x CRE_CUTOFF]
                     [-y ACF_CUTOFF] [-t THREAD] [-n NPROC]

Find the recommended optimal kmer using acf,cre and ofc for a given set of
genomes. Example: kitsune kopt genomeList.txt -kl 15 --canonical --fast -t 4
-o out.txt

optional arguments:
  -h, --help            show this help message and exit
  --filenames FILENAMES
                        A file that list the path to all genomes (fasta
                        format) with extension as (.txt, .csv, .tab) or no
                        extension (default: None)
  --fast                Jellyfish one-pass calculation (faster) (default:
                        False)
  --canonical           Jellyfish count only canonical mer (default: False)
  -kl KLARGE, --klarge KLARGE
                        Largest k-mer length to consider, note: the smallest
                        kmer length is 4 (default: None)
  -o OUTPUT, --output OUTPUT
                        Output filename (default: None)
  --closely_related     For closely related set of genomes, use this option
                        (default: False)
  -x CRE_CUTOFF, --cre_cutoff CRE_CUTOFF
                        Cutoff to use in selecting kmers whose cre's are <=
                        (cutoff * max(cre)), Default = 10 percent, ie x=0.1
                        (default: 0.1)
  -y ACF_CUTOFF, --acf_cutoff ACF_CUTOFF
                        Cutoff to use in selecting kmers whose acf's are >=
                        (cutoff * max(acf)), Default = 10 percent, ie y=0.1
                        (default: 0.1)
  -t THREAD, --thread THREAD
                        Number of threads (default: 1)
  -n NPROC, --nproc NPROC
                        Number of processes (default: 1)

Example dataset

First download the example files. Download

kitsune kopt --filenames genome_list -kl 15 --canonical --fast -t 4 -n 1 -o out.txt

:warning: Please be aware that this command will use big computational resources when large number of genomes and/or large genome size are used as the input.