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Spike detection and automatic clustering for spike sorting

Project description

# Klusta: automatic spike sorting up to 64 channels

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[klusta]( is an open source package for automatic spike sorting of multielectrode neurophysiological recordings made with probes containing up to a few dozens of sites.

We are also working actively on more sophisticated algorithms that will scale to hundreds/thousands of channels. This work is being done within the [phy project](, which is still experimental at this point.

## Overview

klusta implements the following features:

  • Kwik: An HDF5-based file format that stores the results of a spike sorting session.

  • Spike detection (also known as SpikeDetekt): an algorithm designed for probes containing tens of channels, based on a flood-fill algorithm in the adjacency graph formed by the recording sites in the probe.

  • Automatic clustering (also known as Masked KlustaKwik): an automatic clustering algorithm designed for high-dimensional structured datasets.

## GUI

You will need a GUI to visualize the spike sorting results.

We have developed two GUI programs with the same features:

  • phy KwikGUI: newer project, scales to hundreds/thousands of channels, still relatively experimental. It will be automatically installed if you follow the install instructions below.

  • [KlustaViewa]( widely used, but older and a bit hard to install since it relies on very old dependencies.

Both GUIs work with the same Kwik format.

## Quick install guide

The following instructions will install both klusta and the phy KwikGUI.

  1. Make sure that you have [miniconda]( installed. You can choose the Python 3.5 64-bit version for your operating system (Linux, Windows, or OS X).

  2. [Download the environment file.](

  3. Open a terminal (on Windows, cmd, not Powershell) in the directory where you saved the file and type:

    `bash conda env create -n klusta -f environment.yml `

  4. Done! Now, to use klusta and the phy KwikGUI, enter the directory that contains your files and type:

    `bash source activate klusta # omit the `source` on Windows klusta yourfile.prm # spikesort your data with a PRM file phy kwik-gui yourfile.kwik # open the GUI `

    See the documentation for more details.

### Updating the software

To get the latest version of the software, open a terminal and type:

` source activate klusta # omit the `source` on Windows pip install klusta phy phycontrib --upgrade `

## Technical details

klusta is written in pure Python. The clustering code, written in Python and Cython, currently lives in [another repository](

## Links

## Credits

klusta is developed by [Cyrille Rossant](, [Shabnam Kadir](, [Dan Goodman](, [Max Hunter](, and [Kenneth Harris](, in the [Cortexlab](, University College London.

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