In silico serotyping of Listeria monocytogenes
Project description
LisSero
In silico serogroup typing prediction for Listeria monocytogenes
Authors
- Jason Kwong (@kwongjc) - GitHub: kwongj
- Josh Zhang (@abcdtree) - GitHub: abcdtree
- Torsten Seemann (@torstenseemann) - GitHub: tseemann
Maintainers
- MDU PHL - Josh Zhang, Kristy Horan, and Anders Gonçalves da Silva
Dependencies
Installation
pip3 install lissero
Installing BLAST 2.10.0
Brew:
brew install blast
Conda:
conda install -c bioconda blast
In silico serogrouping for Listeria monocytogenes
LisSero is based on a method of predicting serogroup for Listeria monocytogenes using PCR, as described by Doumith et al (see References).
It detects the presence or absence of 5 genes (lmo1118, lmo0737, ORF2110, ORF2819 and Prs).
The patterns obtained reflect the four main serotypes (1/2a, 1/2b, 1/2c, and 4b) obtained from food and human sources.
The patterns are not based on genes involved in somatic (O) or flagellar (H) biosynthesis, and are not specific to just one serotype, but rather to a group of serotypes.
Serogroup | lmo1118 | lmo0737 | ORF2110 | ORF2819 | Prs |
---|---|---|---|---|---|
1/2a, 3a | - | + | - | - | + |
1/2b, 3b, 7 | - | - | - | + | + |
1/2c, 3c | + | + | - | - | + |
4b, 4d, 4e | - | - | + | + | + |
4b, 4d, 4e* | - | + | + | + | + |
Listeria spp. | + |
If only Prs is detected, these isolates are often serotype 4a or 4c, though LisSero reports these as "Nontypable".
Usage
$ lissero --help
Usage: lissero [OPTIONS] FASTA...
In silico serogroup prediction for L. monocytogenes. Alleles: lmo1118,
lmo0737, ORF2819, ORF2110, Prs
References:
* Doumith et al. Differentiation of the major Listeria monocytogenes
serovars by multiplex PCR. J Clin Microbiol, 2004; 42:8; 3819-22
Options:
-s, --serotype_db TEXT
--min_id FLOAT Minimum percent identity to accept a match. [0-100]
[default: 95.0]
--min_cov FLOAT Minimum coverage of the gene to accept a match.
[0-100] [default: 95.0]
--logfile TEXT Save log to a file instead of printing to stderr
--debug
--help
Example usage
lissero my_fasta.fa
Example output
ID | SEROTYPE | PRS | LMO0737 | LMO1118 | ORF2110 | ORF2819 | COMMENT |
---|---|---|---|---|---|---|---|
/path/to/my_fasta.fa | 4b, 4d, 4e | FULL | NONE | NONE | FULL | FULL |
Output explained
The output consist of 8 columns:
- ID: At the moment the full path to your file
- SEROTYPE: A predicted serotype if possible, otherwise
Nontypeable
- Five columns for each of the genes with either:
FULL
: for a complete match to the gene in the database (as defined by the--min_cov
and--min_id
flags which default to at least 95% coverage and percent id)NONE
: when there is no match to the databasePARTIAL
: where there are matches that fall below the--min_cov
and--min_id
thresholds
- COMMENTS: Which will try to explain a
Nontypeable
results. Current cases are:No Prs found
: when there is noFULL
match to thePrs
gene is not likely to be a Listeria monocytogenesPresence of all 5 genes, not a known serotype
: when all 5 genes are presentNo combination of fully matched genes resulted in a known serotype
: An unknown combination- In addition, it will say
Unusual 4b with lmo0737
when reporting4b, 4d, 4e*
serotype
Change Log
Version 0.4.1
- Implemented unit test for all possible 32 possible gene patterns
- Implemented more verbose output with presence/absence info for each gene
- Implemented test of FASTA file, and will exit with warning if a file does not appear to be a FASTA file
- Implement test of whether an input file exists or not
- Implemented logging using Loguru
- Added option to log to a file (
--logfile
; stderr remains default) - Logging now includes the BLAST command used
- Removed all references in the code to Binary Typing
- Implemented
--version
flag - Added some better docs
Version 0.4.0
- Removed support for Binary Typing
- Consolidated repos in MDU-PHL
- Changed executable from
run_lissero
tolissero
Bugs
Please submit via the GitHub issues page: https://github.com/MDU-PHL/LisSero/issues
Software Licence
GPLv2: https://github.com/MDU-PHL/LisSero/blob/master/LICENSE
References
- Doumith et al. Differentiation of the major Listeria monocytogenes serovars by multiplex PCR. J Clin Microbiol, 2004; 42:8; 3819-22.
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