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A nucleotide melt temp calculator

Project description

A nucleotide sequence melt temp calculator for Python.



$ pip install melt

## Usage

From the command line:

$ Tm --dna 200 --na 50 --mg 3 --dntp 0.8 ATGCATGC

As a library:

>>> import melting
>>> melting.temp("ATGCATGC")
>>> melting.temp("ATGCATGC", DNA_c=200, Na_c=50, Mg_c=3, dNTPs_c=0.8)

## Acknowledgements

This code is largely built on existing code from:

Salt concentration equations adapted from IDT, with work from Erik Clarke, Sesh Sundararaman, and Calvin Morrison.

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