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Python3 version of command line scripts of MFEprimer-2.0


Using pip:

pip install mfeprimer-py3

Using git (optional):

git clone
cd mfeprimer-py3
python3 install


# Index the database test/test.rna
# Run MFEprimer -i test/p.fa -d test/test.rna

Check original repo ( for further details about features, outcomes and license agreement.


  • Wubin Qu, Yang Zhou, Yanchun Zhang, Yiming Lu, Xiaolei Wang, Dongsheng Zhao, Yi Yang and Chenggang Zhang*. MFEprimer-2.0: A fast thermodynamics-based program for checking PCR primer specificity. Nucleic Acids Res. 2012 (accepted).

  • Wubin Qu, Zhiyong Shen, Dongsheng Zhao, Yi Yang and Chenggang Zhang. (2009) MFEprimer: multiple factor evaluation of the specificity of PCR primers, Bioinformatics, 25(2), 276-278.

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