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(Python) utility to filter mgz volumes to per-voxel-value directories of jpg/png image slices

Project description

Quick Overview

Recursively walk down a directory tree and extract MGZ labels, creating image slices for each individual label preserving directory structure in output tree.

Overview

mgz2imagetree is a simple Python utility that recursively walks down an inputdir, extracts all the mgz files, filters “labels” from mgz volume files and saves each label set as slices of (by default) png files (using the Python utility called mgz2imgslices), organized into a series of directories, one per label set for each subject, and replicates the entire direcotry structure in the outputdir.

This utility also allows to pass all the CLI arguments that are specific to mgz2imgslices. NOTE:

An mgz format file simply contains a 3D volume data structure of image values. Often these values are interpreted to be image intensities. Sometimes, however, they can be interpreted as label identifiers. Regardless of the interpretation, the volume image data is simply a number value in each voxel of the volume.

Dependencies

Make sure that the following dependencies are installed on your host system (or even better, a python3 virtual env):

pfmisc : (a general miscellaneous module for color support, etc) nibabel : (to read NIfTI/MGZ files) numpy : (to support large, multidimensional arrays and matrices) imageio : (interface to read and write image data) pftree : Create a dictionary representation of a filesystem hierarchy.

Assumptions

This document assumes UNIX conventions and a bash shell. The script should work fine under Windows, but we have not actively tested on that platform – our dev envs are Linux Ubuntu and macOS.

Installation

Python module

One method of installing this script and all of its dependencies is by fetching it from PyPI.

pip3 install mgz2imagetree

How to Use

mgz2imagetree needs at a minimum the following required command line arguments:

  • [-I|--inputDir <inputDir>] : Input directory to examine. By default, the first file in this directory is examined for its tag information.

  • [-O|--outputDir <outputDir>] : The output root directory that will contain a tree structure identical to the input directory, and each “leaf” node will contain the analysis results.

  • [-o|--outputFileStem <outputFileStem>] : The output file stem to store data. This should not have a file extension, or rather, any “.” in the name are considered part of the stem and are not considered extensions.

  • [--feature <MGZFileToConvertToLabelledSegments>] : The feature file containing the cortical strip which needs to be filtered into it constituent cortical segments using mgz2imgslices

  • [--imageFile <MGZFileImageFile>] : The raw 3D mgz image file that needs to be split and stored as slices in the corresponding subject’s output directory.

Examples

First, let’s create a directory, say devel wherever you feel like it. We will place some test data in this directory to process with this plugin.

cd ~/
mkdir devel
cd devel
export DEVEL=$(pwd)

Now, we need to fetch sample MGZ data.

Pull mgz data

git clone https://github.com/FNNDSC/mgz_converter_dataset.git

Make sure the mgz_converter_dataset directory is placed in the devel directory.

  • Make sure your current working directory is devel. At this juncture it should contain mgz_converter_dataset.

  • Create an output directory named results in devel.

mkdir results && chmod 777 results

EXAMPLE 1

  • Run mgz2imgslices using the following command. Change the arguments according to your need.

mgz2imagetree
    --inputDir ${DEVEL}/mgz_converter_dataset/                             \
    --outputDir ${DEVEL}/results/                                          \
    --feature aparc.a2009s+aseg.mgz                                        \
    --imageFile brain.mgz                                                      \
    --outputFileStem sample                                                \
    --outputFileType jpg                                                   \
    --label label                                                          \
    --wholeVolume FullVolume                                               \
    --rawDirName RawImageDirectory                                         \

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