Small RNA-seq annotation
Project description
mirtop
---------
[![](https://travis-ci.org/miRTop/mirtop.svg?branch=master)](https://travis-ci.org/miRTop/mirtop#)
[![Project Status: WIP - Initial development is in progress, but there has not yet been a stable, usable release suitable for the public.](http://www.repostatus.org/badges/latest/wip.svg)](http://www.repostatus.org/#wip)
Command line tool to annotate with a standard naming miRNAs e isomiRs.
See more at: [isomiRs naming discussion](https://github.com/miRTop/incubator/blob/master/isomirs/isomir_naming.md)
Cite
---------
http://mirtop.github.io
About
-----
Join the team: https://orgmanager.miguelpiedrafita.com/join/15463928
Read more: http://mirtop.github.io
Installation
------------
Thes best solution is to install conda to get an independent enviroment.
`wget http://repo.continuum.io/miniconda/Miniconda-latest-Linux-x86_64.sh`
`bash Miniconda-latest-Linux-x86_64.sh -b -p ~/mirtop_env`
`export PATH=$PATH:~/mirtop_env`
`git clone http://github.com/miRTop/mirtop`
`cd mirtop`
`python setup.py develop`
Quick start
-----------
`cd data`
`mirtop gff --sps hsa --hairpin examples/annotate/hairpin.fa --mirna examples/annotate/hsa.gff3 -o test_out_mirs_fasta examples/annotate/sim_isomir.sam
Output
------
There will be a `*.mirna` file that is a tabular file with this format:
Naming of isomiRs follow these rules: https://github.com/miRTop/incubator/blob/master/format/definition.md
Contributors
------------
* [Lorena Pantano](https://github.com/lpantano) (Bioinformatic Core, Harvard Chan School, Boston, USA)
---------
[![](https://travis-ci.org/miRTop/mirtop.svg?branch=master)](https://travis-ci.org/miRTop/mirtop#)
[![Project Status: WIP - Initial development is in progress, but there has not yet been a stable, usable release suitable for the public.](http://www.repostatus.org/badges/latest/wip.svg)](http://www.repostatus.org/#wip)
Command line tool to annotate with a standard naming miRNAs e isomiRs.
See more at: [isomiRs naming discussion](https://github.com/miRTop/incubator/blob/master/isomirs/isomir_naming.md)
Cite
---------
http://mirtop.github.io
About
-----
Join the team: https://orgmanager.miguelpiedrafita.com/join/15463928
Read more: http://mirtop.github.io
Installation
------------
Thes best solution is to install conda to get an independent enviroment.
`wget http://repo.continuum.io/miniconda/Miniconda-latest-Linux-x86_64.sh`
`bash Miniconda-latest-Linux-x86_64.sh -b -p ~/mirtop_env`
`export PATH=$PATH:~/mirtop_env`
`git clone http://github.com/miRTop/mirtop`
`cd mirtop`
`python setup.py develop`
Quick start
-----------
`cd data`
`mirtop gff --sps hsa --hairpin examples/annotate/hairpin.fa --mirna examples/annotate/hsa.gff3 -o test_out_mirs_fasta examples/annotate/sim_isomir.sam
Output
------
There will be a `*.mirna` file that is a tabular file with this format:
Naming of isomiRs follow these rules: https://github.com/miRTop/incubator/blob/master/format/definition.md
Contributors
------------
* [Lorena Pantano](https://github.com/lpantano) (Bioinformatic Core, Harvard Chan School, Boston, USA)
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