MRBLEs decoding and analysis package
This project aims to provide a package to find, decode, extract, analyze and MRBLEs using a set of images.
This package provides the tools to: (1) find the MRBLEs in a monochrome brightfield microscopy image; (2) decode the MRBLEs beads by spectral unmxing, using reference spectra, and then spectrally decode the found beads using Iterative Closest Point Matching and Gaussian Mixture Modeling; (3) extract, statistical values of interest in additional fluorescence channels using the morphology of the MRBLEs, their locations, and respective code; (4) analyze affinity information based on titrations of MRBLEs assays.
MRBLEs (Microspheres with Ratiometric Barcode Lanthanide Encoding) rely on spectral multiplexing to track analytes throughout an experiment. In these assays, we can create microspheres containing > 1,000 unique ratios of lanthanide nanophosphors that can be uniquely identified via imaging alone. We are currently developing new assays that use these microspheres to understand how signaling proteins recognize their peptide substrates and to improve our ability to extract information from single cells.
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|Filename, size & hash SHA256 hash help||File type||Python version||Upload date|
|mrbles-0.11.8-py2.py3-none-any.whl (62.9 kB) Copy SHA256 hash SHA256||Wheel||3.6||Jul 29, 2018|
|mrbles-0.11.8.tar.gz (44.5 kB) Copy SHA256 hash SHA256||Source||None||Jul 29, 2018|