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Software package that identifies raw signal changes between two conditions from https://github.com/jts/nanopolish resquiggled dRNA-Seq data.

Project description

Nanocompore


GitHub license Python BioRxiv

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Nanocompore identifies differences in ONT nanopore sequencing raw signal corresponding to RNA modifications by comparing 2 samples

Nanocompore compares 2 ONT nanopore direct RNA sequencing datasets from different experimental conditions expected to have a significant impact on RNA modifications. It is recommended to have at least 2 replicates per condition. For example one can use a control condition with a significantly reduced number of modifications such as a cell line for which a modification writing enzyme was knocked-down or knocked-out. Alternatively, on a smaller scale transcripts of interests could be synthesized in-vitro.

Full documentation is available at http://nanocompore.rna.rocks

Nanocompore

Companion repositories

Main authors

  • Tommaso Leonardi - tom {at} tleo.io
  • Adrien Leger - aleg {at} ebi.ac.uk

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