nanoraw 0.1

Analysis of nanopore sequencing data.

Summary

This package provides tools for the analysis of raw nanopore sequencing data, including correction of basecalls and visualization.

Requirements

• HDF5 (http://micro.stanford.edu/wiki/Install_HDF5#Install)

• graphmap (https://github.com/isovic/graphmap)

python Requirements:

• python

• numpy

• h5py

• rpy2

R Requirements:

• R

• ggplot2 (not actually enfored on install, but plotting commands will fail; install with install.packages('ggplot2') from an R prompt)

Optionally:

• changepoint (for using R’s changepoint package for re-segmentation)

• Biopython (for robust FASTA parsing, but a simple parser is provided)

Installation

Install nanoraw via pip

pip install nanoraw

Install bleeding edge via github

pip install git+https://github.com/marcus1487/nanoraw.git

Usage

nanoraw -h
nanoraw [command] [options]

Main comands:

• correct: Correct annotation of raw signal with genomic aignement of existing basecalls

• write_wiggle: Write wiggle file of genome coverage.

Plotting comands:

• plot_signal: Plot signal across selected genomic regions.

• plot_comparison: Plot comparison of two read groups.

• plot_kmer: Plot signal quantiles acorss kmers.

  Get additional help for subcommands with nanoraw [command] -h

Legal

nanoraw v.1 Copyright (c) 2016, The Regents of the University of California, through Lawrence Berkeley National Laboratory (subject to receipt of any required approvals from the U.S. Dept. of Energy). All rights reserved.

If you have questions about your rights to use or distribute this software, please contact Berkeley Lab’s Innovation and Partnerships department at [e-mail] referring to “ nanoraw v.1 (2016-199).”

NOTICE. This software was developed under funding from the U.S. Department of Energy. As such, the U.S. Government has been granted for itself and others acting on its behalf a paid-up, nonexclusive, irrevocable, worldwide license in the Software to reproduce, prepare derivative works, and perform publicly and display publicly. Beginning five (5) years after the date permission to assert copyright is obtained from the U.S. Department of Energy, and subject to any subsequent five (5) year renewals, the U.S. Government is granted for itself and others acting on its behalf a paid-up, nonexclusive, irrevocable, worldwide license in the Software to reproduce, prepare derivative works, distribute copies to the public, perform publicly and display publicly, and to permit others to do so.