nf-core

Helper tools for use with nf-core Nextflow pipelines.

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Maintainers

alneberg apeltzer ewels sven1103

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Project description

A python package with helper tools for the nf-core community.

Read this documentation on the nf-core website: https://nf-co.re/tools

nf-core tools installation
nf-core list - List available pipelines
nf-core launch - Run a pipeline with interactive parameter prompts
nf-core download - Download pipeline for offline use
nf-core licences - List software licences in a pipeline
nf-core create - Create a new pipeline with the nf-core template
nf-core lint - Check pipeline code against nf-core guidelines
nf-core schema - Work with pipeline schema files
nf-core bump-version - Update nf-core pipeline version number
nf-core sync - Synchronise pipeline TEMPLATE branches
nf-core modules - commands for dealing with DSL2 modules
Citation

The nf-core tools package is written in Python and can be imported and used within other packages. For documentation of the internal Python functions, please refer to the Tools Python API docs.

Installation

Bioconda

You can install nf-core/tools from bioconda.

First, install conda and configure the channels to use bioconda (see the bioconda documentation). Then, just run the conda installation command:

conda install nf-core

Alternatively, you can create a new environment with both nf-core/tools and nextflow:

conda create --name nf-core python=3.7 nf-core nextflow
conda activate nf-core

Python Package Index

nf-core/tools can also be installed from PyPI using pip as follows:

pip install nf-core

Development version

If you would like the latest development version of tools, the command is:

pip install --upgrade --force-reinstall git+https://github.com/nf-core/tools.git@dev

If you intend to make edits to the code, first make a fork of the repository and then clone it locally. Go to the cloned directory and install with pip (also installs development requirements):

pip install --upgrade -r requirements-dev.txt -e .

Using a specific Python interpreter

If you prefer, you can also run tools with a specific Python interpreter. The command line usage and flags are then exactly the same as if you ran with the nf-core command. Note that the module is nf_core with an underscore, not a hyphen like the console command.

For example:

python -m nf_core --help
python3 -m nf_core list
~/my_env/bin/python -m nf_core create --name mypipeline --description "This is a new skeleton pipeline"

Using with your own Python scripts

The tools functionality is written in such a way that you can import it into your own scripts. For example, if you would like to get a list of all available nf-core pipelines:

import nf_core.list
wfs = nf_core.list.Workflows()
wfs.get_remote_workflows()
for wf in wfs.remote_workflows:
    print(wf.full_name)

Please see https://nf-co.re/tools-docs/ for the function documentation.

Automatic version check

nf-core/tools automatically checks the web to see if there is a new version of nf-core/tools available. If you would prefer to skip this check, set the environment variable NFCORE_NO_VERSION_CHECK. For example:

export NFCORE_NO_VERSION_CHECK=1

Listing pipelines

The command nf-core list shows all available nf-core pipelines along with their latest version, when that was published and how recently the pipeline code was pulled to your local system (if at all).

An example of the output from the command is as follows:

$ nf-core list

                                          ,--./,-.
          ___     __   __   __   ___     /,-._.--~\
    |\ | |__  __ /  ` /  \ |__) |__         }  {
    | \| |       \__, \__/ |  \ |___     \`-._,-`-,
                                          `._,._,'

    nf-core/tools version 1.13

┏━━━━━━━━━━━━━━━━━━━┳━━━━━━━┳━━━━━━━━━━━━━━━━┳━━━━━━━━━━━━━━━┳━━━━━━━━━━━━━━┳━━━━━━━━━━━━━━━━━━━━━━━┓
┃ Pipeline Name     ┃ Stars ┃ Latest Release ┃      Released ┃  Last Pulled ┃ Have latest release?  ┃
┡━━━━━━━━━━━━━━━━━━━╇━━━━━━━╇━━━━━━━━━━━━━━━━╇━━━━━━━━━━━━━━━╇━━━━━━━━━━━━━━╇━━━━━━━━━━━━━━━━━━━━━━━┩
│ rnafusion         │    45 │          1.2.0 │   2 weeks ago │            - │ -                     │
│ hic               │    17 │          1.2.1 │   3 weeks ago │ 4 months ago │ No (v1.1.0)           │
│ chipseq           │    56 │          1.2.0 │   4 weeks ago │  4 weeks ago │ No (dev - bfe7eb3)    │
│ atacseq           │    40 │          1.2.0 │   4 weeks ago │  6 hours ago │ No (master - 79bc7c2) │
│ viralrecon        │    20 │          1.1.0 │  1 months ago │ 1 months ago │ Yes (v1.1.0)          │
│ sarek             │    59 │          2.6.1 │  1 months ago │            - │ -                     │
[..truncated..]

To narrow down the list, supply one or more additional keywords to filter the pipelines based on matches in titles, descriptions and topics:

$ nf-core list rna rna-seq

                                          ,--./,-.
          ___     __   __   __   ___     /,-._.--~\
    |\ | |__  __ /  ` /  \ |__) |__         }  {
    | \| |       \__, \__/ |  \ |___     \`-._,-`-,
                                          `._,._,'

    nf-core/tools version 1.13

┏━━━━━━━━━━━━━━━┳━━━━━━━┳━━━━━━━━━━━━━━━━┳━━━━━━━━━━━━━━┳━━━━━━━━━━━━━┳━━━━━━━━━━━━━━━━━━━━━━┓
┃ Pipeline Name ┃ Stars ┃ Latest Release ┃     Released ┃ Last Pulled ┃ Have latest release? ┃
┡━━━━━━━━━━━━━━━╇━━━━━━━╇━━━━━━━━━━━━━━━━╇━━━━━━━━━━━━━━╇━━━━━━━━━━━━━╇━━━━━━━━━━━━━━━━━━━━━━┩
│ dualrnaseq    │     3 │          1.0.0 │ 1 months ago │           - │ -                    │
│ rnaseq        │   304 │            3.0 │ 3 months ago │ 1 years ago │ No (v1.4.2)          │
│ rnafusion     │    56 │          1.2.0 │ 8 months ago │ 2 years ago │ No (v1.0.1)          │
│ smrnaseq      │    18 │          1.0.0 │  1 years ago │           - │ -                    │
│ circrna       │     1 │            dev │            - │           - │ -                    │
│ lncpipe       │    18 │            dev │            - │           - │ -                    │
│ scflow        │     2 │            dev │            - │           - │ -                    │
└───────────────┴───────┴────────────────┴──────────────┴─────────────┴──────────────────────┘

You can sort the results by latest release (-s release, default), when you last pulled a local copy (-s pulled), alphabetically (-s name), or number of GitHub stars (-s stars).

$ nf-core list -s stars

                                          ,--./,-.
          ___     __   __   __   ___     /,-._.--~\
    |\ | |__  __ /  ` /  \ |__) |__         }  {
    | \| |       \__, \__/ |  \ |___     \`-._,-`-,
                                          `._,._,'

    nf-core/tools version 1.13

┏━━━━━━━━━━━━━━━━━━━┳━━━━━━━┳━━━━━━━━━━━━━━━━┳━━━━━━━━━━━━━━━┳━━━━━━━━━━━━━━┳━━━━━━━━━━━━━━━━━━━━━━━┓
┃ Pipeline Name     ┃ Stars ┃ Latest Release ┃      Released ┃  Last Pulled ┃ Have latest release?  ┃
┡━━━━━━━━━━━━━━━━━━━╇━━━━━━━╇━━━━━━━━━━━━━━━━╇━━━━━━━━━━━━━━━╇━━━━━━━━━━━━━━╇━━━━━━━━━━━━━━━━━━━━━━━┩
│ rnaseq            │   207 │          1.4.2 │  9 months ago │   5 days ago │ Yes (v1.4.2)          │
│ sarek             │    59 │          2.6.1 │  1 months ago │            - │ -                     │
│ chipseq           │    56 │          1.2.0 │   4 weeks ago │  4 weeks ago │ No (dev - bfe7eb3)    │
│ methylseq         │    47 │            1.5 │  4 months ago │            - │ -                     │
│ rnafusion         │    45 │          1.2.0 │   2 weeks ago │            - │ -                     │
│ ampliseq          │    41 │          1.1.2 │  7 months ago │            - │ -                     │
│ atacseq           │    40 │          1.2.0 │   4 weeks ago │  6 hours ago │ No (master - 79bc7c2) │
[..truncated..]

To return results as JSON output for downstream use, use the --json flag.

Archived pipelines are not returned by default. To include them, use the --show_archived flag.

Launch a pipeline

Some nextflow pipelines have a considerable number of command line flags that can be used. To help with this, you can use the nf-core launch command You can choose between a web-based graphical interface or an interactive command-line wizard tool to enter the pipeline parameters for your run. Both interfaces show documentation alongside each parameter and validate your inputs.

The tool uses the nextflow_schema.json file from a pipeline to give parameter descriptions, defaults and grouping. If no file for the pipeline is found, one will be automatically generated at runtime.

Nextflow params variables are saved in to a JSON file called nf-params.json and used by nextflow with the -params-file flag. This makes it easier to reuse these in the future.

The command takes one argument - either the name of an nf-core pipeline which will be pulled automatically, or the path to a directory containing a Nextflow pipeline (can be any pipeline, doesn't have to be nf-core).

$ nf-core launch rnaseq

                                          ,--./,-.
          ___     __   __   __   ___     /,-._.--~\
    |\ | |__  __ /  ` /  \ |__) |__         }  {
    | \| |       \__, \__/ |  \ |___     \`-._,-`-,
                                          `._,._,'

    nf-core/tools version 1.13


INFO     This tool ignores any pipeline parameter defaults overwritten by Nextflow config files or profiles

INFO     Using local workflow: nf-core/rnaseq (v3.0)
INFO     [✓] Default parameters look valid
INFO     [✓] Pipeline schema looks valid (found 85 params)
INFO     Would you like to enter pipeline parameters using a web-based interface or a command-line wizard?
? Choose launch method  Command line


?  Nextflow command-line flags
General Nextflow flags to control how the pipeline runs.
These are not specific to the pipeline and will not be saved in any parameter file. They are just used when building the nextflow run launch command.
(Use arrow keys)

 » Continue >>
   ---------------
   -name
   -profile
   -work-dir  [./work]
   -resume  [False]

Once complete, the wizard will ask you if you want to launch the Nextflow run. If not, you can copy and paste the Nextflow command with the nf-params.json file of your inputs.

INFO     [✓] Input parameters look valid
INFO     Nextflow command:
         nextflow run nf-core/rnaseq -params-file "nf-params.json"


Do you want to run this command now?  [y/n]:

Launch tool options

-r, --revision
- Specify a pipeline release (or branch / git commit sha) of the project to run
-i, --id
- You can use the web GUI for nf-core pipelines by clicking "Launch" on the website. Once filled in you will be given an ID to use with this command which is used to retrieve your inputs.
-c, --command-only
- If you prefer not to save your inputs in a JSON file and use -params-file, this option will specify all entered params directly in the nextflow command.
-p, --params-in PATH
- To use values entered in a previous pipeline run, you can supply the nf-params.json file previously generated.
- This will overwrite the pipeline schema defaults before the wizard is launched.
-o, --params-out PATH
- Path to save parameters JSON file to. (Default: nf-params.json)
-a, --save-all
- Without this option the pipeline will ignore any values that match the pipeline schema defaults.
- This option saves all parameters found to the JSON file.
-h, --show-hidden
- A pipeline JSON schema can define some parameters as 'hidden' if they are rarely used or for internal pipeline use only.
- This option forces the wizard to show all parameters, including those labelled as 'hidden'.
--url
- Change the URL used for the graphical interface, useful for development work on the website.

Downloading pipelines for offline use

Sometimes you may need to run an nf-core pipeline on a server or HPC system that has no internet connection. In this case you will need to fetch the pipeline files first, then manually transfer them to your system.

To make this process easier and ensure accurate retrieval of correctly versioned code and software containers, we have written a download helper tool. Simply specify the name of the nf-core pipeline and it will be downloaded to your current working directory.

By default, the pipeline will download the pipeline code and the institutional nf-core/configs files. If you specify the flag --singularity, it will also download any singularity image files that are required.

Use -r/--release to download a specific release of the pipeline. If not specified, the tool will automatically fetch the latest release.

$ nf-core download rnaseq -r 3.0 --singularity

                                          ,--./,-.
          ___     __   __   __   ___     /,-._.--~\
    |\ | |__  __ /  ` /  \ |__) |__         }  {
    | \| |       \__, \__/ |  \ |___     \`-._,-`-,
                                          `._,._,'

    nf-core/tools version 1.13



INFO     Saving rnaseq
          Pipeline release: '3.0'
          Pull singularity containers: 'Yes'
          Output file: 'nf-core-rnaseq-3.0.tar.gz'
INFO     Downloading workflow files from GitHub
INFO     Downloading centralised configs from GitHub
INFO     Fetching container names for workflow
INFO     Found 29 containers
INFO     Tip: Set env var $NXF_SINGULARITY_CACHEDIR to use a central cache for container downloads
Downloading singularity images ━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━ 100% • 29/29 completed
INFO     Compressing download..
INFO     Command to extract files: tar -xzf nf-core-rnaseq-3.0.tar.gz
INFO     MD5 checksum for nf-core-rnaseq-3.0.tar.gz: 9789a9e0bda50f444ab0ee69cc8a95ce

The tool automatically compresses all of the resulting file in to a .tar.gz archive. You can choose other formats (.tar.bz2, zip) or to not compress (none) with the -c/--compress flag. The console output provides the command you need to extract the files.

Once uncompressed, you will see something like the following file structure for the downloaded pipeline:

$ tree -L 2 nf-core-methylseq-1.4/

nf-core-rnaseq-3.0
├── configs
│   ├── ..truncated..
│   ├── nextflow.config
│   ├── nfcore_custom.config
│   └── pipeline
├── singularity-images
│   ├── containers.biocontainers.pro-s3-SingImgsRepo-biocontainers-v1.2.0_cv1-biocontainers_v1.2.0_cv1.img.img
│   ├── ..truncated..
│   └── depot.galaxyproject.org-singularity-umi_tools-1.1.1--py38h0213d0e_1.img
└── workflow
    ├── CHANGELOG.md
    ├── ..truncated..
    └── main.nf

You can run the pipeline by simply providing the directory path for the workflow folder to your nextflow run command.

Downloaded nf-core configs

The pipeline files are automatically updated (params.custom_config_base is set to ../configs), so that the local copy of institutional configs are available when running the pipeline. So using -profile <NAME> should work if available within nf-core/configs.

Downloading singularity containers

If you're using Singularity, the nf-core download command can also fetch the required Singularity container images for you. To do this, specify the --singularity option. Your archive / target output directory will then include three folders: workflow, configs and also singularity-containers.

The downloaded workflow files are again edited to add the following line to the end of the pipeline's nextflow.config file:

singularity.cacheDir = "${projectDir}/../singularity-images/"

This tells Nextflow to use the singularity-containers directory relative to the workflow for the singularity image cache directory. All images should be downloaded there, so Nextflow will use them instead of trying to pull from the internet.

Singularity cache directory

We highly recommend setting the $NXF_SINGULARITY_CACHEDIR environment variable on your system, even if that is a different system to where you will be running Nextflow.

If found, the tool will fetch the Singularity images to this directory first before copying to the target output archive / directory. Any images previously fetched will be found there and copied directly - this includes images that may be shared with other pipelines or previous pipeline version downloads or download attempts.

If you are running the download on the same system where you will be running the pipeline (eg. a shared filesystem where Nextflow won't have an internet connection at a later date), you can choose specify --singularity-cache. This instructs nf-core download to fetch all Singularity images to the $NXF_SINGULARITY_CACHEDIR directory but does not copy them to the workflow archive / directory. The workflow config file is not edited. This means that when you later run the workflow, Nextflow will just use the cache folder directly.

How the Singularity image downloads work

The Singularity image download finds containers using two methods:

It runs nextflow config on the downloaded workflow to look for a process.container statement for the whole pipeline. This is the typical method used for DSL1 pipelines.
It scrapes any files it finds with a .nf file extension in the workflow modules directory for lines that look like container = "xxx". This is the typical method for DSL2 pipelines, which have one container per process.

Some DSL2 modules have container addresses for docker (eg. quay.io/biocontainers/fastqc:0.11.9--0) and also URLs for direct downloads of a Singularity continaer (eg. https://depot.galaxyproject.org/singularity/fastqc:0.11.9--0). Where both are found, the download URL is preferred.

Once a full list of containers is found, they are processed in the following order:

If the target image already exists, nothing is done (eg. with $NXF_SINGULARITY_CACHEDIR and --singularity-cache specified)
If found in $NXF_SINGULARITY_CACHEDIR and --singularity-cache is not specified, they are copied to the output directory
If they start with http they are downloaded directly within Python (default 4 at a time, you can customise this with --parallel-downloads)
If they look like a Docker image name, they are fetched using a singularity pull command
- This requires Singularity to be installed on the system and is substantially slower

Note that compressing many GBs of binary files can be slow, so specifying --compress none is recommended when downloading Singularity images.

If you really like hammering your internet connection, you can set --parallel-downloads to a large number to download loads of images at once.

Pipeline software licences

Sometimes it's useful to see the software licences of the tools used in a pipeline. You can use the licences subcommand to fetch and print the software licence from each conda / PyPI package used in an nf-core pipeline.

NB: Currently this command does not work for DSL2 pipelines. This will be addressed soon.

$ nf-core licences rnaseq

                                          ,--./,-.
          ___     __   __   __   ___     /,-._.--~\
    |\ | |__  __ /  ` /  \ |__) |__         }  {
    | \| |       \__, \__/ |  \ |___     \`-._,-`-,
                                          `._,._,'

    nf-core/tools version 1.10

  INFO     Fetching licence information for 25 tools
  INFO     Warning: This tool only prints licence information for the software tools packaged using conda.
  INFO     The pipeline may use other software and dependencies not described here.
┏━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━┳━━━━━━━━━┳━━━━━━━━━━━━━━━━━━━━━━┓
┃ Package Name                      ┃ Version ┃ Licence              ┃
┡━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━╇━━━━━━━━━╇━━━━━━━━━━━━━━━━━━━━━━┩
│ stringtie                         │ 2.0     │ Artistic License 2.0 │
│ bioconductor-summarizedexperiment │ 1.14.0  │ Artistic-2.0         │
│ preseq                            │ 2.0.3   │ GPL                  │
│ trim-galore                       │ 0.6.4   │ GPL                  │
│ bioconductor-edger                │ 3.26.5  │ GPL >=2              │
│ fastqc                            │ 0.11.8  │ GPL >=3              │
│ bioconductor-tximeta              │ 1.2.2   │ GPLv2                │
│ qualimap                          │ 2.2.2c  │ GPLv2                │
│ r-gplots                          │ 3.0.1.1 │ GPLv2                │
│ r-markdown                        │ 1.1     │ GPLv2                │
│ rseqc                             │ 3.0.1   │ GPLv2                │
│ bioconductor-dupradar             │ 1.14.0  │ GPLv3                │
│ deeptools                         │ 3.3.1   │ GPLv3                │
│ hisat2                            │ 2.1.0   │ GPLv3                │
│ multiqc                           │ 1.7     │ GPLv3                │
│ salmon                            │ 0.14.2  │ GPLv3                │
│ star                              │ 2.6.1d  │ GPLv3                │
│ subread                           │ 1.6.4   │ GPLv3                │
│ r-base                            │ 3.6.1   │ GPLv3.0              │
│ sortmerna                         │ 2.1b    │ LGPL                 │
│ gffread                           │ 0.11.4  │ MIT                  │
│ picard                            │ 2.21.1  │ MIT                  │
│ samtools                          │ 1.9     │ MIT                  │
│ r-data.table                      │ 1.12.4  │ MPL-2.0              │
│ matplotlib                        │ 3.0.3   │ PSF-based            │
└───────────────────────────────────┴─────────┴──────────────────────┘

Creating a new pipeline

The create subcommand makes a new pipeline using the nf-core base template. With a given pipeline name, description and author, it makes a starter pipeline which follows nf-core best practices.

After creating the files, the command initialises the folder as a git repository and makes an initial commit. This first "vanilla" commit which is identical to the output from the templating tool is important, as it allows us to keep your pipeline in sync with the base template in the future. See the nf-core syncing docs for more information.

$ nf-core create

                                          ,--./,-.
          ___     __   __   __   ___     /,-._.--~\
    |\ | |__  __ /  ` /  \ |__) |__         }  {
    | \| |       \__, \__/ |  \ |___     \`-._,-`-,
                                          `._,._,'

    nf-core/tools version 1.13

Workflow Name: nextbigthing
Description: This pipeline analyses data from the next big 'omics technique
Author: Big Steve
  INFO     Creating new nf-core pipeline: nf-core/nextbigthing
  INFO     Initialising pipeline git repository
  INFO     Done. Remember to add a remote and push to GitHub:
            cd /Users/philewels/GitHub/nf-core/tools/test-create/nf-core-nextbigthing
            git remote add origin git@github.com:USERNAME/REPO_NAME.git
            git push --all origin
  INFO     This will also push your newly created dev branch and the TEMPLATE branch for syncing.
  INFO     !!!!!! IMPORTANT !!!!!!

           If you are interested in adding your pipeline to the nf-core community,
           PLEASE COME AND TALK TO US IN THE NF-CORE SLACK BEFORE WRITING ANY CODE!

           Please read: https://nf-co.re/developers/adding_pipelines#join-the-community

Once you have run the command, create a new empty repository on GitHub under your username (not the nf-core organisation, yet) and push the commits from your computer using the example commands in the above log. You can then continue to edit, commit and push normally as you build your pipeline.

Please see the nf-core documentation for a full walkthrough of how to create a new nf-core workflow.

As the log output says, remember to come and discuss your idea for a pipeline as early as possible! See the documentation for instructions.

Note that if the required arguments for nf-core create are not given, it will interactively prompt for them. If you prefer, you can supply them as command line arguments. See nf-core create --help for more information.

Linting a workflow

The lint subcommand checks a given pipeline for all nf-core community guidelines. This is the same test that is used on the automated continuous integration tests.

For example, the current version looks something like this:

$ cd path/to/my_pipeline
$ nf-core lint .

                                          ,--./,-.
          ___     __   __   __   ___     /,-._.--~\
    |\ | |__  __ /  ` /  \ |__) |__         }  {
    | \| |       \__, \__/ |  \ |___     \`-._,-`-,
                                          `._,._,'
    nf-core/tools version 1.13


  INFO     Testing pipeline: nf-core-testpipeline/
╭──────────────────────────────────────────────────────────────────────────────────────────────────────────╮
│ [!] 3 Test Warnings                                                                                      │
├──────────────────────────────────────────────────────────────────────────────────────────────────────────┤
│ actions_awsfulltest: .github/workflows/awsfulltest.yml should test full datasets, not -profile test      │
│ conda_env_yaml: Conda dep outdated: bioconda::fastqc=0.11.8, 0.11.9 available                            │
│ conda_env_yaml: Conda dep outdated: bioconda::multiqc=1.7, 1.9 available                                 │
╰──────────────────────────────────────────────────────────────────────────────────────────────────────────╯
╭───────────────────────╮
│ LINT RESULTS SUMMARY  │
├───────────────────────┤
│ [✔] 155 Tests Passed  │
│ [?]   0 Tests Ignored │
│ [!]   3 Test Warnings │
│ [✗]   0 Tests Failed  │
╰───────────────────────╯

Tip: Some of these linting errors can automatically be resolved with the following command:

    nf-core lint . --fix conda_env_yaml

Linting documentation

Each test result name on the left is a terminal hyperlink. In most terminals you can ctrl + click ( cmd + click) these links to open documentation specific to this test in your browser.

Alternatively visit https://nf-co.re/tools-docs/lint_tests/index.html and find your test to read more.

Linting config

It's sometimes desirable to disable certain lint tests, especially if you're using nf-core/tools with your own pipeline that is outside of nf-core.

To help with this, you can add a linting config file to your pipeline called .nf-core-lint.yml or .nf-core-lint.yaml in the pipeline root directory. Here you can list the names of any tests that you would like to disable and set them to False, for example:

actions_awsfulltest: False
pipeline_todos: False

Some lint tests allow greater granularity, for example skipping a test only for a specific file. This is documented in the test-specific docs but generally involves passing a list, for example:

files_exist:
  - CODE_OF_CONDUCT.md
files_unchanged:
  - assets/email_template.html
  - CODE_OF_CONDUCT.md

Automatically fix errors

Some lint tests can try to automatically fix any issues they find. To enable this functionality, use the --fix flag. The pipeline must be a git repository with no uncommitted changes for this to work. This is so that any automated changes can then be reviewed and undone (git checkout .) if you disagree.

Lint results output

The output from nf-core lint is designed to be viewed on the command line and is deliberately succinct. You can view all passed tests with --show-passed or generate JSON / markdown results with the --json and --markdown flags.

Pipeline schema

nf-core pipelines have a nextflow_schema.json file in their root which describes the different parameters used by the workflow. These files allow automated validation of inputs when running the pipeline, are used to generate command line help and can be used to build interfaces to launch pipelines. Pipeline schema files are built according to the JSONSchema specification (Draft 7).

To help developers working with pipeline schema, nf-core tools has three schema sub-commands:

nf-core schema validate
nf-core schema build
nf-core schema lint

Validate pipeline parameters

Nextflow can take input parameters in a JSON or YAML file when running a pipeline using the -params-file option. This command validates such a file against the pipeline schema.

Usage is nextflow schema validate <pipeline> <parameter file>, eg:

$ nf-core schema validate rnaseq nf-params.json

                                          ,--./,-.
          ___     __   __   __   ___     /,-._.--~\
    |\ | |__  __ /  ` /  \ |__) |__         }  {
    | \| |       \__, \__/ |  \ |___     \`-._,-`-,
                                          `._,._,'

    nf-core/tools version 1.13



INFO     Using local workflow: nf-core/rnaseq (v3.0)
INFO     [✓] Default parameters look valid
INFO     [✓] Pipeline schema looks valid (found 85 params)
INFO     [✓] Input parameters look valid

The pipeline option can be a directory containing a pipeline, a path to a schema file or the name of an nf-core pipeline (which will be downloaded using nextflow pull).

Build a pipeline schema

Manually building JSONSchema documents is not trivial and can be very error prone. Instead, the nf-core schema build command collects your pipeline parameters and gives interactive prompts about any missing or unexpected params. If no existing schema is found it will create one for you.

Once built, the tool can send the schema to the nf-core website so that you can use a graphical interface to organise and fill in the schema. The tool checks the status of your schema on the website and once complete, saves your changes locally.

Usage is nextflow schema build <pipeline_directory>, eg:

$ nf-core schema build nf-core-testpipeline

                                          ,--./,-.
          ___     __   __   __   ___     /,-._.--~\
    |\ | |__  __ /  ` /  \ |__) |__         }  {
    | \| |       \__, \__/ |  \ |___     \`-._,-`-,
                                          `._,._,'

    nf-core/tools version 1.13

  INFO     [✓] Default parameters look valid
  INFO     [✓] Pipeline schema looks valid (found 25 params)
❓ Unrecognised 'params.old_param' found in schema but not pipeline! Remove it? [y/n]: y
❓ Unrecognised 'params.we_removed_this_too' found in schema but not pipeline! Remove it? [y/n]: y
✨ Found 'params.input' in pipeline but not in schema. Add to pipeline schema? [y/n]: y
✨ Found 'params.outdir' in pipeline but not in schema. Add to pipeline schema? [y/n]: y
  INFO     Writing schema with 25 params: 'nf-core-testpipeline/nextflow_schema.json'
🚀 Launch web builder for customisation and editing? [y/n]: y
  INFO: Opening URL: https://nf-co.re/pipeline_schema_builder?id=1234567890_abc123def456
  INFO: Waiting for form to be completed in the browser. Remember to click Finished when you're done.
  INFO: Found saved status from nf-core JSON Schema builder
  INFO: Writing JSON schema with 25 params: nf-core-testpipeline/nextflow_schema.json

There are three flags that you can use with this command:

--no-prompts: Make changes without prompting for confirmation each time. Does not launch web tool.
--web-only: Skips comparison of the schema against the pipeline parameters and only launches the web tool.
--url <web_address>: Supply a custom URL for the online tool. Useful when testing locally.

Linting a pipeline schema

The pipeline schema is linted as part of the main pipeline nf-core lint command, however sometimes it can be useful to quickly check the syntax of the JSONSchema without running a full lint run.

Usage is nextflow schema lint <schema>, eg:

$ nf-core schema lint nextflow_schema.json

                                          ,--./,-.
          ___     __   __   __   ___     /,-._.--~\
    |\ | |__  __ /  ` /  \ |__) |__         }  {
    | \| |       \__, \__/ |  \ |___     \`-._,-`-,
                                          `._,._,'

    nf-core/tools version 1.13

  ERROR    [✗] Pipeline schema does not follow nf-core specs:
            Definition subschema 'input_output_options' not included in schema 'allOf'

Bumping a pipeline version number

When releasing a new version of a nf-core pipeline, version numbers have to be updated in several different places. The helper command nf-core bump-version automates this for you to avoid manual errors (and frustration!).

The command uses results from the linting process, so will only work with workflows that pass these tests.

Usage is nf-core bump-version <pipeline_dir> <new_version>, eg:

$ cd path/to/my_pipeline
$ nf-core bump-version . 1.7
                                          ,--./,-.
          ___     __   __   __   ___     /,-._.--~\
    |\ | |__  __ /  ` /  \ |__) |__         }  {
    | \| |       \__, \__/ |  \ |___     \`-._,-`-,
                                          `._,._,'

    nf-core/tools version 1.13



INFO     Changing version number from '1.6dev' to '1.7'
INFO     Updated version in 'nextflow.config'
           - version = '1.6dev'
           + version = '1.7'
           - process.container = 'nfcore/methylseq:dev'
           + process.container = 'nfcore/methylseq:1.7'


INFO     Updated version in '.github/workflows/ci.yml'
           - run: docker build --no-cache . -t nfcore/methylseq:dev
           + run: docker build --no-cache . -t nfcore/methylseq:1.7
           - docker tag nfcore/methylseq:dev nfcore/methylseq:dev
           + docker tag nfcore/methylseq:dev nfcore/methylseq:1.7


INFO     Updated version in 'environment.yml'
           - name: nf-core-methylseq-1.6dev
           + name: nf-core-methylseq-1.7


INFO     Updated version in 'Dockerfile'
           - ENV PATH /opt/conda/envs/nf-core-methylseq-1.6dev/bin:$PATH
           + ENV PATH /opt/conda/envs/nf-core-methylseq-1.7/bin:$PATH
           - RUN conda env export --name nf-core-methylseq-1.6dev > nf-core-methylseq-1.6dev.yml
           + RUN conda env export --name nf-core-methylseq-1.7 > nf-core-methylseq-1.7.yml

To change the required version of Nextflow instead of the pipeline version number, use the flag --nextflow.

Sync a pipeline with the template

Over time, the main nf-core pipeline template is updated. To keep all nf-core pipelines up to date, we synchronise these updates automatically when new versions of nf-core/tools are released. This is done by maintaining a special TEMPLATE branch, containing a vanilla copy of the nf-core template with only the variables used when it first ran (name, description etc.). This branch is updated and a pull-request can be made with just the updates from the main template code.

Note that pipeline synchronisation happens automatically each time nf-core/tools is released, creating an automated pull-request on each pipeline. As such, you do not normally need to run this command yourself!

This command takes a pipeline directory and attempts to run this synchronisation. Usage is nf-core sync <pipeline_dir>, eg:

$ nf-core sync my_pipeline/
                                          ,--./,-.
          ___     __   __   __   ___     /,-._.--~\
    |\ | |__  __ /  ` /  \ |__) |__         }  {
    | \| |       \__, \__/ |  \ |___     \`-._,-`-,
                                          `._,._,'

    nf-core/tools version 1.13



INFO     Pipeline directory: /path/to/my_pipeline/
INFO     Original pipeline repository branch is 'master'
INFO     Deleting all files in 'TEMPLATE' branch
INFO     Making a new template pipeline using pipeline variables
INFO     Committed changes to 'TEMPLATE' branch
INFO     Checking out original branch: 'master'
INFO     Now try to merge the updates in to your pipeline:
           cd /path/to/my_pipeline/
           git merge TEMPLATE

The sync command tries to check out the TEMPLATE branch from the origin remote or an existing local branch called TEMPLATE. It will fail if it cannot do either of these things. The nf-core create command should make this template automatically when you first start your pipeline. Please see the nf-core website sync documentation if you have difficulties.

By default, the tool will collect workflow variables from the current branch in your pipeline directory. You can supply the --from-branch flag to specific a different branch.

Finally, if you give the --pull-request flag, the command will push any changes to the remote and attempt to create a pull request using the GitHub API. The GitHub username and repository name will be fetched from the remote url (see git remote -v | grep origin), or can be supplied with --username and --repository.

To create the pull request, a personal access token is required for API authentication. These can be created at https://github.com/settings/tokens. Supply this using the --auth-token flag.

Modules

With the advent of Nextflow DSL2, we are creating a centralised repository of modules. These are software tool process definitions that can be imported into any pipeline. This allows multiple pipelines to use the same code for share tools and gives a greater degree of granulairy and unit testing.

The nf-core DSL2 modules repository is at https://github.com/nf-core/modules

List modules

To list all modules available on nf-core/modules, you can use nf-core modules list, which will print all available modules to the terminal.

$ nf-core modules list
                                          ,--./,-.
          ___     __   __   __   ___     /,-._.--~\
    |\ | |__  __ /  ` /  \ |__) |__         }  {
    | \| |       \__, \__/ |  \ |___     \`-._,-`-,
                                          `._,._,'

    nf-core/tools version 1.13


INFO     Modules available from nf-core/modules (master)

┏━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━┓
┃ Module Name                    ┃
┡━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━┩
│ bandage/image                  │
│ bcftools/consensus             │
│ bcftools/filter                │
│ bcftools/isec                  │
│ bcftools/merge                 │
│ bcftools/mpileup               │
│ bcftools/stats                 │
│ ..truncated..                  │
└────────────────────────────────┘

List installed modules

The same nf-core modules list command can take an optional argument for a local pipeline directory. If given, it will instead list all installed modules in that pipeline.

Install a module into a pipeline

You can install modules from nf-core/modules in your pipeline using nf-core modules install <pipeline_dir>. A module installed this way will be installed to the <pipeline_dir>/modules/nf-core/software directory.

$ nf-core modules install .
                                          ,--./,-.
          ___     __   __   __   ___     /,-._.--~\
    |\ | |__  __ /  ` /  \ |__) |__         }  {
    | \| |       \__, \__/ |  \ |___     \`-._,-`-,
                                          `._,._,'

    nf-core/tools version 1.13

? Tool name: cat/fastq
INFO     Installing cat/fastq
INFO     Downloaded 3 files to ./modules/nf-core/software/cat/fastq

Use the --tool flat to specify a module name on the command line instead of using the cli prompt.

Remove a module from a pipeline

To delete a module from your pipeline, run nf-core modules remove <pipeline-directory>

$ nf-core modules remove .

                                          ,--./,-.
          ___     __   __   __   ___     /,-._.--~\
    |\ | |__  __ /  ` /  \ |__) |__         }  {
    | \| |       \__, \__/ |  \ |___     \`-._,-`-,
                                          `._,._,'

    nf-core/tools version 1.13

? Tool name: star/align
INFO     Removing star/align
INFO     Successfully removed star/align module

Create a new module

This command creates a new nf-core module from the nf-core module template. This ensures that your module follows the nf-core guidelines. The template contains extensive TODO messages to walk you through the changes you need to make to the template.

You can create a new module using nf-core modules create <directory>.

If writing a module for the shared nf-core/modules repository, the <directory> argument should be the path to the clone of your fork of the modules repository.

Alternatively, if writing a more niche module that does not make sense to share, <directory> should be the path to your pipeline.

The nf-core modules create command will prompt you with the relevant questions in order to create all of the necessary module files.

$ nf-core modules create .

                                          ,--./,-.
          ___     __   __   __   ___     /,-._.--~\
    |\ | |__  __ /  ` /  \ |__) |__         }  {
    | \| |       \__, \__/ |  \ |___     \`-._,-`-,
                                          `._,._,'

    nf-core/tools version 1.13


INFO     Press enter to use default values (shown in brackets) or type your own responses. ctrl+click underlined text to open links.
Name of tool/subtool: star/align
INFO     Using Bioconda package: 'bioconda::star=2.6.1d'
INFO     Using Docker / Singularity container with tag: 'star:2.6.1d--0'
GitHub Username: (@ewels):
INFO     Provide an appropriate resource label for the process, taken from the nf-core pipeline template.
         For example: process_low, process_medium, process_high, process_long
? Process resource label: process_high
INFO     Where applicable all sample-specific information e.g. 'id', 'single_end', 'read_group' MUST be provided as an input via a
         Groovy Map called 'meta'. This information may not be required in some instances, for example indexing reference genome files.
Will the module require a meta map of sample information? (yes/no) [y/n] (y): y
INFO     Created / edited following files:
           ./software/star/align/functions.nf
           ./software/star/align/main.nf
           ./software/star/align/meta.yml
           ./tests/software/star/align/main.nf
           ./tests/software/star/align/test.yml
           ./tests/config/pytest_software.yml

Create a module test config file

All modules on nf-core/modules have a strict requirement of being unit tested using minimal test data. To help developers build new modules, the nf-core modules create-test-yml command automates the creation of the yaml file required to document the output file md5sum and other information generated by the testing. After you have written a minimal Nextflow script to test your module modules/tests/software/<tool>/<subtool>/main.nf, this command will run the tests for you and create the modules/tests/software/<tool>/<subtool>/test.yml file.

$ nf-core modules create-test-yml

                                          ,--./,-.
          ___     __   __   __   ___     /,-._.--~\
    |\ | |__  __ /  ` /  \ |__) |__         }  {
    | \| |       \__, \__/ |  \ |___     \`-._,-`-,
                                          `._,._,'

    nf-core/tools version 1.13


INFO     Press enter to use default values (shown in brackets) or type your own responses
? Tool name: star/align
Test YAML output path (- for stdout) (tests/software/star/align/test.yml):
File exists! 'tests/software/star/align/test.yml' Overwrite? [y/n]: y
INFO     Looking for test workflow entry points: 'tests/software/star/align/main.nf'
──────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────
INFO     Building test meta for entry point 'test_star_alignment_single_end'
Test name (star align test_star_alignment_single_end):
Test command (nextflow run tests/software/star/align -entry test_star_alignment_single_end -c tests/config/nextflow.config):
Test tags (comma separated) (star_alignment_single_end,star_align,star):
Test output folder with results (leave blank to run test):
? Choose software profile  Docker
INFO     Running 'star/align' test with command:
         nextflow run tests/software/star/align -entry test_star_alignment_single_end -c tests/config/nextflow.config --outdir
         /var/folders/bq/451scswn2dn4npxhf_28lyt40000gn/T/tmp_p22f8bg
INFO     Test workflow finished!
──────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────
INFO     Building test meta for entry point 'test_star_alignment_paired_end'
Test name (star align test_star_alignment_paired_end):
Test command (nextflow run tests/software/star/align -entry test_star_alignment_paired_end -c tests/config/nextflow.config):
Test tags (comma separated) (star_align,star_alignment_paired_end,star):
Test output folder with results (leave blank to run test):
INFO     Running 'star/align' test with command:
         nextflow run tests/software/star/align -entry test_star_alignment_paired_end -c tests/config/nextflow.config --outdir
         /var/folders/bq/451scswn2dn4npxhf_28lyt40000gn/T/tmp5qc3kfie
INFO     Test workflow finished!
INFO     Writing to 'tests/software/star/align/test.yml'

Check a module against nf-core guidelines

Run this command to modules in a given directory (pipeline or nf-core/modules clone) against nf-core guidelines.

Use the --all flag to run linting on all modules found.

$ nf-core modules lint nf-core-modules
                                          ,--./,-.
          ___     __   __   __   ___     /,-._.--~\
    |\ | |__  __ /  ` /  \ |__) |__         }  {
    | \| |       \__, \__/ |  \ |___     \`-._,-`-,
                                          `._,._,'

    nf-core/tools version 1.13

? Lint all modules or a single named module?  Named module
? Tool name: star/align
INFO     Linting modules repo: .
INFO     Linting module: star/align
╭──────────────────────────────────────────┬────────────────────────────────────┬─────────────────────────────╮
│ Module name                              │ Test message                       │ File path                   │
├──────────────────────────────────────────┼────────────────────────────────────┼─────────────────────────────┤
│ star/align                               │ Conda update: bioconda::star       │ software/star/align/main.nf │
│                                          │ 2.6.1d -> 2.7.8a                   │                             │
╰──────────────────────────────────────────┴────────────────────────────────────┴─────────────────────────────╯
╭──────────────────────╮
│ LINT RESULTS SUMMARY │
├──────────────────────┤
│ [✔]  18 Tests Passed │
│ [!]   1 Test Warning │
│ [✗]   0 Test Failed  │
╰──────────────────────╯

Citation

If you use nf-core tools in your work, please cite the nf-core publication as follows:

The nf-core framework for community-curated bioinformatics pipelines.

Philip Ewels, Alexander Peltzer, Sven Fillinger, Harshil Patel, Johannes Alneberg, Andreas Wilm, Maxime Ulysse Garcia, Paolo Di Tommaso & Sven Nahnsen.

Nat Biotechnol. 2020 Feb 13. doi: 10.1038/s41587-020-0439-x.

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alneberg apeltzer ewels sven1103

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2.13.1

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nf-core 1.13

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Creating a new pipeline

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Build a pipeline schema

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