OptiNiSt

OptiNiSt(Optical Neuroimage Studio) is a GUI based workflow pipeline tools for processing two-photon calcium imaging data.

OptiNiSt helps researchers try multiple data analysis methods, visualize the results, and construct the data analysis pipelines easily and quickly on GUI. OptiNiSt's data-saving format follows NWB standards.

OptiNiSt also supports reproducibility of scientific research, standardization of analysis protocols, and developments of novel analysis tools as plug-in.

Support Library

ROI detection

• Suite2p
• CaImAn
• LCCD

Postprocessing

• Basic Neural Analysis (Event Trigger Average...)
• Dimension Reduction (PCA...)
• Neural Decoding (LDA...)
• Neural Population Analysis (Correlation...)

Saving Format

• NWB

Key Features

🔰 Easy-To-Create Workflow

• zero-knowledge of coding: OptiNiSt allows you to create analysis pipelines easily on the GUI.

⚡ Visualizing analysis results

• quick visualization: OptiNiSt supports you visualize the analysis results by plotly.

🚀 Managing Workflows

• recording and reproducing: OptiNiSt records and reproduces the workflow pipelines easily.

Installation

Need anaconda or miniconda or miniforge environment.

conda create -n optinist python=3.9
conda activate optinist

Install from pip.

pip install optinist

launch.

run_optinist

Open browser. http://localhost:8000

Documentation

https://optinist.readthedocs.io/en/latest/

Using GUI

Workflow

• OptiNiSt allows you to make your analysis pipelines by graph style using nodes and edges on GUI. Parameters for each analysis are easily changeable.

Visualize

• OptiNiSt allows you to visualize the analysis results with one click by plotly. It supports a variety of plotting styles.

Record

• OptiNiSt supports you in recording and reproducing workflow pipelines in an organized manner.

Contributors

Proposers

Kenji Doya, Yukako Yamane OIST Neural Computation Unit

Main Developers

Shogo Akiyama, Yoshifumi Takeshima

Support Developers

Tatsuya Tanabe, Yosuke Kaneko, Syuya Saeki

References

[Suite2p] Marius Pachitariu, Carsen Stringer, Mario Dipoppa, Sylvia Schröder, L. Federico Rossi, Henry Dalgleish, Matteo Carandini, Kenneth D. Harris. "Suite2p: beyond 10,000 neurons with standard two-photon microscopy". 2017 [CaImAn] Andrea Giovannucci Is a corresponding author, Johannes Friedrich, Pat Gunn, Jérémie Kalfon, Brandon L Brown, Sue Ann Koay, Jiannis Taxidis, Farzaneh Najafi, Jeffrey L Gauthier, Pengcheng Zhou, Baljit S Khakh, David W Tank, Dmitri B Chklovskii, Eftychios A Pnevmatikakis. "CaImAn: An open source tool for scalable Calcium Imaging data Analysis". 2019 [LCCD] Tsubasa Ito, Keisuke Ota, Kanako Ueno, Yasuhiro Oisi, Chie Matsubara, Kenta Kobayashi, Masamichi Ohkura, Junichi Nakai, Masanori Murayama, Toru Aonishi, "Low computational-cost cell detection method for calcium imaging data", 2022 [PyNWB] Oliver Rübel, Andrew Tritt, Ryan Ly, Benjamin K. Dichter, Satrajit Ghosh, Lawrence Niu, Ivan Soltesz, Karel Svoboda, Loren Frank, Kristofer E. Bouchard, "The Neurodata Without Borders ecosystem for neurophysiological data science", bioRxiv 2021.03.13.435173, March 15, 2021

Citation

If you use this software, please cite our paper: https://journals.plos.org/ploscompbiol/article?id=10.1371/journal.pcbi.1013087

@misc{OptiNiSt,
	author = {Yamane, Yukako and Li, Yuzhe and Matsumoto, Keita and Kanai, Ryota and Desforges, Miles and Gutierrez, Carlos Enrique and Doya, Kenji},
	title = {Optical Neuroimage Studio (OptiNiSt): intuitive, scalable, extendable framework for optical neuroimage data analysis},
	volume = {21},
	issue = {5},
	year = {2025},
	doi = {10.1371/journal.pcbi.1013087},
  journal = {PLOS Computational Biology}
}

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