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Bioinformatics pipelines for HTS data

Project description

The PALEOMIX pipelines are a set of pipelines and tools designed to aid the rapid processing of High-Throughput Sequencing (HTS) data: The BAM pipeline processes de-multiplexed reads from one or more samples, through sequence processing and alignment, to generate BAM alignment files useful in downstream analyses; the Phylogenetic pipeline carries out genotyping and phylogenetic inference on BAM alignment files, either produced using the BAM pipeline or generated elsewhere; and the Zonkey pipeline carries out a suite of analyses on low coverage equine alignments, in order to detect the presence of F1-hybrids in archaeological assemblages. In addition, PALEOMIX aids in metagenomic analysis of the extracts.

The pipelines have been designed with ancient DNA (aDNA) in mind, and includes several features especially useful for the analyses of ancient samples, but can all be for the processing of modern samples, in order to ensure consistent data processing.

Installation and usage

Detailed instructions can be found in the documentation for PALEOMIX. For questions, bug reports, and/or suggestions, please use the GitHub tracker or contact Mikkel Schubert at


The PALEOMIX pipelines have been published in Nature Protocols; if you make use of PALEOMIX in your work, then please cite

Schubert M, Ermini L, Sarkissian CD, Jónsson H, Ginolhac A, Schaefer R, Martin MD, Fernández R, Kircher M, McCue M, Willerslev E, and Orlando L. “Characterization of ancient and modern genomes by SNP detection and phylogenomic and metagenomic analysis using PALEOMIX”. Nat Protoc. 2014 May;9(5):1056-82. doi: 10.1038/nprot.2014.063. Epub 2014 Apr 10. PubMed PMID: 24722405.

The Zonkey pipeline has been published in Journal of Archaeological Science; if you make use of this pipeline in your work, then please cite

Schubert M, Mashkour M, Gaunitz C, Fages A, Seguin-Orlando A, Sheikhi S, Alfarhan AH, Alquraishi SA, Al-Rasheid KAS, Chuang R, Ermini L, Gamba C, Weinstock J, Vedat O, and Orlando L. “Zonkey: A simple, accurate and sensitive pipeline to genetically identify equine F1-hybrids in archaeological assemblages”. Journal of Archaeological Science. 2017 Feb; 78:147-157. doi: 10.1016/j.jas.2016.12.005.

Project details

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