paleomix 1.2.5

Bioinformatics pipelines for HTS data

The PALEOMIX pipeline is a set of pipelines and tools designed to aid the rapid processing of High-Throughput Sequencing (HTS) data, starting from de-multiplexed reads from one or more samples, through sequence processing and alignment, followed by genotyping and phylogenetic inference on the samples. In addition, PALEOMIX aids in metagenomic analysis of the extracts. The pipeline has been designed with ancient DNA (aDNA) in mind, and includes several features especially useful for the analyses of ancient samples, but can be of used for both modern and ancient samples to ensure consistent data processing.

For a detailed description of the pipeline, please refer to PALEOMIX website and the documentation; for questions, bug reports, and/or suggestions, use the GitHub tracker, or contact Mikkel Schubert at [e-mail].

The PALEOMIX pipeline has been published in Nature Protocols; if you make use of (parts of) the pipeline in your work, then please cite

Schubert M, Ermini L, Sarkissian CD, Jónsson H, Ginolhac A, Schaefer R, Martin MD, Fernández R, Kircher M, McCue M, Willerslev E, and Orlando L. “Characterization of ancient and modern genomes by SNP detection and phylogenomic and metagenomic analysis using PALEOMIX”. Nat Protoc. 2014 May;9(5):1056-82. doi: 10.1038/nprot.2014.063. Epub 2014 Apr 10. PubMed PMID: 24722405.