pioreactor-air-bubbler 0.4.0

Add an air bubbler to your Pioreactor as a background job

Pioreactor air bubbler

Add an air-pump / bubbler to your Pioreactor. This pump can run continuously, or if OD reading is running, will stop during a reading.

Usage

pio run air_bubbler

Installation

Software

From the command line, run:

pio install-plugin pioreactor_air_bubbler

(Optional) Edit the following to your config.ini

[PWM]
<the PWM channel you pick>=air_bubbler


[air_bubbler]
duty_cycle=10
hertz=200
pre_delay_duration=1.5
post_delay_duration=0.75
enable_dodging_od=1

Hardware

1. Connect the PWM channel to the air pump's power source.
2. Connect a tube between the air pump and a tube in the vial's cap, via luer lock.
3. The connecting tube in the vial cap can be pushed into the liquid for bubbling, or left in the headspace to exchange air.
4. Optional: a 0.22 micron filter can be placed along the air path to filter contaminants.