Create PCR primers optimized for length, tm, gc and free energy
Project description
primers
from primers import pcr, Primer
# create a FWD and REV primer
ps = pcr("ATGGATGGTAGAGATAGATGG",
add_fwd="GGTAGGTAGAT", add_rev="GGTTTTAGGATAGAT", add_min=10, add_max=25,
opt_tm=55.0, opt_len=30)
ps[0] # Primer("GGTAGGTAGATATGGATGGT", tm=62.3)
ps[1] # Primer("GGTTTTAGGATAGATCCTACTATCT", tm=64.0)
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