Extract promoter sequence for biologists
Project description
Overview
The promoterExtract is python package for nucleotide manipulation in bioinformatics. The package contains two subcommands including create and extract. The create subcommand is used for creating database and extract subcomand is used for extracting promoter sequence. Both two subcomand is executed through get_promoter command in command line. Argument -l means the length of promoter, int type. Argument -u utr5 after TSS, int type. Argument -f reference genome fasta of a specific organism. Argument -g annotation file including GTF and GFF. Argument -o means output file path.
Brief introduction of format package
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Install
pip install promoterExtract # other git clone https://github.com/SitaoZ/promoterExtract.git cd promoterExtract; python setup.py install
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Usage
which get_promoter get_promoter -h get_promoter create -h get_promoter extract -h
# step 1 get_promoter create -g ath.gff3 # step 2 get_promoter extract -l 200 -u 100 -f ath.fa -g gff.db -o promoter.csv
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