Overview
The BEDTools suite of programs is widely used for genomic interval manipulation or “genome algebra”. pybedtools wraps and extends BEDTools and offers feature-level manipulations from within Python.
See full online documentation, including installation instructions, at https://daler.github.io/pybedtools/.
The GitHub repo is at https://github.com/daler/pybedtools.
Why pybedtools?
Here is an example to get the names of genes that are <5 kb away from intergenic SNPs:
from pybedtools import BedTool
snps = BedTool('snps.bed.gz') # [1]
genes = BedTool('hg19.gff') # [1]
intergenic_snps = snps.subtract(genes) # [2]
nearby = genes.closest(intergenic_snps, d=True, stream=True) # [2, 3]
for gene in nearby: # [4]
if int(gene[-1]) < 5000: # [4]
print gene.name # [4]Useful features shown here include:
[1] support for all BEDTools-supported formats (here gzipped BED and GFF)
[2] wrapping of all BEDTools programs and arguments (here, subtract and closest and passing the -d flag to closest);
[3] streaming results (like Unix pipes, here specified by stream=True)
[4] iterating over results while accessing feature data by index or by attribute access (here [-1] and .name).
In contrast, here is the same analysis using shell scripting. Note that this requires knowledge in Perl, bash, and awk. The run time is identical to the pybedtools version above:
snps=snps.bed.gz
genes=hg19.gff
intergenic_snps=/tmp/intergenic_snps
snp_fields=`zcat $snps | awk '(NR == 2){print NF; exit;}'`
gene_fields=9
distance_field=$(($gene_fields + $snp_fields + 1))
intersectBed -a $snps -b $genes -v > $intergenic_snps
closestBed -a $genes -b $intergenic_snps -d \
| awk '($'$distance_field' < 5000){print $9;}' \
| perl -ne 'm/[ID|Name|gene_id]=(.*?);/; print "$1\n"'
rm $intergenic_snpsSee the Shell script comparison in the docs for more details on this comparison, or keep reading the full documentation at http://daler.github.io/pybedtools.
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Metadata
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Release files for pybedtools 0.12.1
For a detailed explanation of source distributions (sdists) and built distributions (wheels), please see the package formats documentation.
Source distribution (sdist)
| File | Size | Uploaded | |
|---|---|---|---|
| pybedtools-0.12.1.tar.gz | 12.7 MB | Details |
Built distributions (wheels)
Total release size: 533.7 MB
Release files / pybedtools-0.12.1.tar.gz
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Release files / pybedtools-0.12.1-cp310-cp310-musllinux_1_2_x86_64.whl
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Release files / pybedtools-0.12.1-cp310-cp310-musllinux_1_2_aarch64.whl
| Download URL | pybedtools-0.12.1-cp310-cp310-musllinux_1_2_aarch64.whl |
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| Size | 15.6 MB |
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Release files / pybedtools-0.12.1-cp310-cp310-manylinux_2_24_x86_64.manylinux_2_28_x86_64.whl
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Release files / pybedtools-0.12.1-cp310-cp310-manylinux_2_24_aarch64.manylinux_2_28_aarch64.whl
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| Size | 14.7 MB |
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Release files / pybedtools-0.12.1-cp310-cp310-macosx_11_0_arm64.whl
| Download URL | pybedtools-0.12.1-cp310-cp310-macosx_11_0_arm64.whl |
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Release files / pybedtools-0.12.1-cp310-cp310-macosx_10_9_x86_64.whl
| Download URL | pybedtools-0.12.1-cp310-cp310-macosx_10_9_x86_64.whl |
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Release files / pybedtools-0.12.1-cp39-cp39-musllinux_1_2_x86_64.whl
| Download URL | pybedtools-0.12.1-cp39-cp39-musllinux_1_2_x86_64.whl |
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| Size | 15.8 MB |
| Tags | CPython 3.9 Linux musl 1.2+ x86-64 |
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No |
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Release files / pybedtools-0.12.1-cp39-cp39-musllinux_1_2_aarch64.whl
| Download URL | pybedtools-0.12.1-cp39-cp39-musllinux_1_2_aarch64.whl |
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Release files / pybedtools-0.12.1-cp39-cp39-manylinux_2_24_x86_64.manylinux_2_28_x86_64.whl
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Release files / pybedtools-0.12.1-cp39-cp39-manylinux_2_24_aarch64.manylinux_2_28_aarch64.whl
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| Size | 14.7 MB |
| Tags | CPython 3.9 Linux glibc 2.24+ ARM64 Linux glibc 2.28+ ARM64 |
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No |
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twine/7.0.0 CPython/3.12.12
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Release files / pybedtools-0.12.1-cp39-cp39-macosx_11_0_arm64.whl
| Download URL | pybedtools-0.12.1-cp39-cp39-macosx_11_0_arm64.whl |
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No |
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Release files / pybedtools-0.12.1-cp39-cp39-macosx_10_9_x86_64.whl
| Download URL | pybedtools-0.12.1-cp39-cp39-macosx_10_9_x86_64.whl |
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| Size | 12.9 MB |
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No |
| Uploaded via |
twine/7.0.0 CPython/3.12.12
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